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Brilliant Violet 650™ anti-mouse CD3 Antibody

Pricing & Availability
Clone
17A2 (See other available formats)
Regulatory Status
RUO
Other Names
T cell antigen receptor complex, T3
Isotype
Rat IgG2b, κ
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Product Citations
publications
17A2_BV650_051012
C57BL/6 mouse splenocytes were stained with CD19 APC and CD3 (clone 17A2) Brilliant Violet 650™.
  • 17A2_BV650_051012
    C57BL/6 mouse splenocytes were stained with CD19 APC and CD3 (clone 17A2) Brilliant Violet 650™.
Compare all formats See Brilliant Violet 650™ spectral data
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100229 125 µL 176€
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Description

CD3, also known as T3, is a member of the Ig superfamily and primarily expressed on T cells, NK-T cells, and at different levels on thymocytes during T cell differentiation. CD3 is composed of CD3ε, δ, γ and ζ chains. It forms a TCR complex by associating with TCR α/β or γ/δ chains. CD3 plays a critical role in TCR signal transduction, T cell activation, and antigen recognition by binding the peptide/MHC antigen complex

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
γδTCR-positive T-T hybridoma D1
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 650™ under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 650™ excites at 405 nm and emits at 645 nm. The bandpass filter 660/20 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 650™ is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

Additional reported application (for relevant formats) include: spatial biology (IBEX)1,2.

Application References
  1. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  2. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Komuczki J, et al. 2019. Immunity. 50:1289. PubMed
  2. Yamin R, et al. 2021. Nature. 599:465. PubMed
  3. Mirchandani AS, et al. 2022. Nat Immunol. 23:927. PubMed
  4. Edwards SC, et al. 2023. J Exp Med. 220: . PubMed
  5. Briukhovetska D, et al. 2023. Immunity. 56:143. PubMed
  6. Whyte CE, et al. 2022. Curr Protoc. 2:e589. PubMed
  7. Dijkman K, et al. 2023. NPJ Vaccines. 8:66. PubMed
  8. Bhaskar A, et al. 2023. iScience. 26:106644. PubMed
  9. Oh J, et al. 2023. Nat Commun. 14:3278. PubMed
  10. Trittel S, et al. 2019. Sci Rep. 9:16362. PubMed
  11. Larsen SE, et al. 2021. Sci Rep. 11:9040. PubMed
  12. Clemmensen HS, et al. 2021. MBio. 12:. PubMed
  13. Yoshihara E, et al. 2020. Nature. 606:586. PubMed
  14. Bhattacherjee A, et al. 2019. Commun Biol. 2:450. PubMed
  15. Cao W, et al. 2017. Immunity. 47:1182. PubMed
  16. Woodworth JS, et al. 2021. Nat Commun. 12:6658. PubMed
  17. Clemmensen HS, et al. 2020. Front Immunol. 11:585359. PubMed
  18. Dikiy S, et al. 2021. Immunity. 54(5):931-946.e11. PubMed
  19. Sophie Thiemann et al. 2017. Cell host & microbe. 21(6):682-694 . PubMed
  20. Lissner MM, et al. 2020. Elife. 9:00. PubMed
  21. André P et al. 2018. Cell. 175(7):1731-1743 . PubMed
  22. Xu J et al. 2018. Cell. 173(3):762-775 . PubMed
  23. Mamedov MR, et al. 2018. Immunity. 48:350. PubMed
  24. Campbell C et al. 2018. Immunity. 48(6):1245-1257 . PubMed
  25. Grauel AL, et al. 2020. Nat Commun. 4.84375. PubMed
  26. Sanmarco LM, et al. 2021. Nature. 590:473. PubMed
  27. Moguche AO et al. 2017. Cell host & microbe. 21(6):695-706 . PubMed
  28. Pasciuto E, et al. 2020. Cell. 182:625. PubMed
  29. Cillo AR, et al. 2021. Cell Rep Med. 2:100476. PubMed
  30. Contreras NA, et al. 2019. PLoS Pathog. 15:e1007890. PubMed
  31. Georgiadou A, et al. 2022. Elife. 11:. PubMed
  32. Filskov J, et al. 2019. Sci Rep. 9:14085. PubMed
  33. Dhayade S, et al. 2020. Nutrients. 12:. PubMed
  34. Demaria O, et al. 2022. Cell Rep Med. 3:100783. PubMed
  35. Pattabiraman G, et al. 2021. Am J Physiol Renal Physiol. . PubMed
  36. McGinley AM, et al. 2020. Immunity. 52(2):342-356. PubMed
  37. Jacobs L, et al. 2022. Cancer Gene Ther. 29:984. PubMed
RRID
AB_11204249 (BioLegend Cat. No. 100229)

Antigen Details

Structure
Ig superfamily, CD3/TCR, 20 kD
Distribution

Thymocytes (differentiation dependent), mature T cells, NK-T cells

Function
Antigen recognition, TCR signal transduction, T cell activation
Ligand/Receptor
Peptide antigen/MHC-complex
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Davis MM. 1990. Annu. Rev. Biochem. 59:475.
3. Weiss A, et al. 1994. Cell 76:263.

Gene ID
12502 View all products for this Gene ID
UniProt
View information about CD3 on UniProt.org

Other Formats

View All CD3 Reagents Request Custom Conjugation
Description Clone Applications
FITC anti-mouse CD3 17A2 FC
PE anti-mouse CD3 17A2 FC
Purified anti-mouse CD3 17A2 FC,IHC-F,IP,ICC
Alexa Fluor® 647 anti-mouse CD3 17A2 FC,IHC-F,3D IHC,SB
Alexa Fluor® 488 anti-mouse CD3 17A2 FC,IHC-F,3D IHC
Pacific Blue™ anti-mouse CD3 17A2 FC
Alexa Fluor® 700 anti-mouse CD3 17A2 FC
PerCP/Cyanine5.5 anti-mouse CD3 17A2 FC
PE/Cyanine7 anti-mouse CD3 17A2 FC
APC/Cyanine7 anti-mouse CD3 17A2 FC
Brilliant Violet 421™ anti-mouse CD3 17A2 FC,ICC
Brilliant Violet 570™ anti-mouse CD3 17A2 FC
Brilliant Violet 650™ anti-mouse CD3 17A2 FC
Brilliant Violet 785™ anti-mouse CD3 17A2 FC
Brilliant Violet 510™ anti-mouse CD3 17A2 FC
APC anti-mouse CD3 17A2 FC
Ultra-LEAF™ Purified anti-mouse CD3 17A2 FC,IHC-F,IP,ICC
Brilliant Violet 605™ anti-mouse CD3 17A2 FC
Alexa Fluor® 594 anti-mouse CD3 17A2 IHC-F,FC,3D IHC,SB
Brilliant Violet 711™ anti-mouse CD3 17A2 FC
Biotin anti-mouse CD3 17A2 FC,IHC-F
PE/Dazzle™ 594 anti-mouse CD3 17A2 FC
APC/Fire™ 750 anti-mouse CD3 17A2 FC
Brilliant Violet 750™ anti-mouse CD3 17A2 FC
TotalSeq™-A0182 anti-mouse CD3 17A2 PG
TotalSeq™-B0182 anti-mouse CD3 17A2 PG
Spark Blue™ 550 anti-mouse CD3 17A2 FC
Spark NIR™ 685 anti-mouse CD3 17A2 FC
TotalSeq™-C0182 anti-mouse CD3 17A2 PG
APC/Fire™ 810 anti-mouse CD3 17A2 FC
PE/Fire™ 640 anti-mouse CD3 17A2 FC
Spark YG™ 570 anti-mouse CD3 17A2 IHC-F
PE/Fire™ 700 anti-mouse CD3 17A2 FC
PE/Cyanine5 anti-mouse CD3 17A2 FC
Spark Blue™ 574 anti-mouse CD3 Antibody 17A2 FC
Spark Violet™ 423 anti-mouse CD3 17A2 FC
PE/Fire™ 810 anti-mouse CD3 17A2 FC
Spark Red™ 718 anti-mouse CD3 17A2 FC
Spark UV™ 387 anti-mouse CD3 17A2 FC
Go To Top Version: 1    Revision Date: 11.30.2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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