Brilliant Violet 785™ anti-human CD184 (CXCR4) Antibody

Product Details

Verified Reactivity

Human, Cynomolgus, Rhesus

Reported Reactivity

African Green, Baboon, Chimpanzee, Sooty Mangabey

Antibody Type

Monoclonal

Host Species

Mouse

Immunogen

CP-MAC-infected Sup-T1 cells

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).

Preparation

The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 785™ under optimal conditions.

Concentration

Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Brilliant Violet 785™ excites at 405 nm and emits at 785 nm. The bandpass filter 780/60 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 785™ is a trademark of Sirigen Group Ltd.

Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.

Excitation Laser

Violet Laser (405 nm)

Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of paraffin-embedded tissue sections¹¹, immunocytochemistry³, immunofluorescence microscopy^2,6, and blocking of CD4-independent infection by HIV-2 and CD4-dependent infection by some T cell-tropic isolates of HIV-1^4,5. Clone 12G5 may not be suitable for Western blotting.¹⁰ The Ultra-LEAF™ purified antibody (Endotoxin <0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 306539 & 306540).

Application References

1. McKnight A, et al. 1997. J. Virol. 71:1692.
2. Endres MJ, et al. 1996. Cell 87:745. (Immunogen, IF)
3. Volin MV, et al. 1998. Biochem. Biophys. Res. Commun. 242:46. (ICC)
4. Berndt C, et al. 1998. P. Natl. Acad. Sci. USA 95:12556. (Block)
5. Ullrich CK, et al. 2000. Blood 96:1438. (Block)
6. Murga M, et al. 2005. Blood 105:1992. (IF)
7. Thompson BD. 2007. J. Biol. Chem. 282:9547. (FC) PubMed
8. Isnardi I, et al. 2010. Blood 115:5026. PubMed
9. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
10. Fischer T, et al. 2008. PLoS One 3:e4069.
11. Schmid BC, et al. 2004. Breast Cancer Res. Treat. 84:247. (IHC)

Product Citations

1. Sparbier CE, et al. 2023. Nat Cell Biol. 25:258. PubMed
2. Takano T, et al. 2023. Nat Commun. 14:1451. PubMed

RRID

AB_2687008 (BioLegend Cat. No. 306529)
AB_2687008 (BioLegend Cat. No. 306530)

Antigen Details

Structure

Rhodopsin family, G-protein linked seven transmembrane glycoprotein, 45 kD

Distribution

T cells and B cells, dendritic cells, monocytes, granulocytes, hematopoietic progenitors, endothelial cells

Function

B lymphopoiesis and myelopoiesis, cardiogenesis, blood vessel formation, cerebellar development

Ligand/Receptor

SDF-1 receptor, coreceptor for X4 HIV-1

Cell Type

B cells, Dendritic cells, Endothelial cells, Granulocytes, Hematopoietic stem and progenitors, Mesenchymal Stem Cells, Monocytes, Neural Stem Cells, T cells, Tregs

Biology Area

Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience, Neuroscience Cell Markers, Stem Cells

Molecular Family

CD Molecules, Cytokine/Chemokine Receptors, GPCR

Antigen References

1. Berger E, et al. 1999. Annu. Rev. Immunol. 17:657.
2. Loetscher P, et al. 2000. Adv. Immunol. 74:127.
3. Murphy P, et al. 2000. Pharmacol. Rev. 52:145.

Gene ID

7852 View all products for this Gene ID

UniProt

View information about CD184 on UniProt.org

Documentation

• Technical Data Sheet (pdf)
• Certificate of Analysis
• Material Safety Data Sheet

Reviews

Related Pages & Pathways

Related FAQs

Does staining at room temperature or even at 37°C help for checking chemokine receptors expression?

Due to continuous recycling of many chemokine receptors, it may be worthwhile to consider staining at room temperature or at 37°C if the staining at lower temperature (which can potentially reduce receptor turnover) is not optimal.

Other Formats

Certificate of Analysis