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Cell Staining Buffer

Pricing & Availability
Regulatory Status
RUO
Other Names
Staining Buffer, Washing Buffer
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Product Citations
262 publications
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420201 500 mL 72€
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Description

Cell Staining Buffer is an antibody diluent and cell wash buffer optimized for use in immunofluorescent staining of viable or fixed single cell suspensions. Cell Staining Buffer contains bovine calf serum as a protein carrier to reduce non-specific binding of antibodies and fluorochrome reagents to target cells. It also contains a metabolic inhibitor, sodium azide (NaN3), to inhibit patching and capping of cell surface antigens. To prevent interference in biotin/avidin indirect staining protocols, Cell Staining Buffer is formulated without biotin, calcium, magnesium, and EDTA.

Product Details
Technical Data Sheet (pdf)

Product Details

Storage & Handling
Store between 2°C and 8°C.
Application

FC - Quality tested
ICC, ICFC - Reported in the literature, not verified in house
Application References
  1. Koon HW, et al. 2013. J Vis exp. 68:4208. PubMed.
  2. Yu DH, et al. 2013. Mol Cell Biol. 33:1845. PubMed.
  3. Garcia JA, et al. 2013. J Immunol. 191:1063. PubMed
  4. Lukas S, et al. 2013. J Biomol Screen. 19:407. PubMed
  5. Murali VK, et al. 2014. J. Virol. 88:903. PubMed
  6. Kusner LL, et al. 2014. PLoS One. 9:102231. PubMed
  7. de Vires M, 2014. Am J Physiol Lung Cell Mol Physiol. 307:240. PubMed
  8. Della-Torre E, et al. 2014. Ann Rheum Dis. PubMed
  9. Dong A, et al. 2015. J Biol Chem. 290:8016. PubMed
Product Citations
  1. Nuccitelli R, et al. 2017. J Immunother Cancer . 10.1186/s40425-017-0234-5. PubMed
  2. Djeungoue–Petga MA, et al. 2019. Cell Death Dis. 1.069444444. PubMed
  3. Markowicz-Piasecka M, et al. 2020. Apoptosis. 25:426. PubMed
  4. Zhuo Y, et al. 2022. J Immunother Cancer. 10:. PubMed
  5. Su CC, et al. 2022. Invest Ophthalmol Vis Sci. 63:18. PubMed
  6. Apfelbaum AA, et al. 2022. Clin Cancer Res. 28:4466. PubMed
  7. Sun Y, et al. 2022. STAR Protoc. 3:101479. PubMed
  8. Brady SW, et al. 2022. Nat Genet. 54:1376. PubMed
  9. Ferrall-Fairbanks MC, et al. 2022. Blood Cancer Discov. 3:536. PubMed
  10. Udayasuryan B, et al. 2022. Sci Signal. 15:eabn4948. PubMed
  11. Xu Y, et al. 2022. Nat Genet. 54:1690. PubMed
  12. Zhong J, et al. 2022. J Adv Res. 41:145. PubMed
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Antigen Details

Cell Type
Tregs
Gene ID
NA
Go To Top Version: 1    Revision Date: 11.30.2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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