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Cell-Vive™ Ultra-LEAF™ Defined Human TruStain FcX™, GMP

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Other Names
Fc Receptor Blocking Solution
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Cell-Vive_CD_Human_TruStain_FcX_GMP_083022
THP-1 cells were treated with Cell-Vive™ Ultra-LEAF™ Defined Human TruStain FcX™, GMP followed by staining with an irrelevant PE mouse IgG2a, κ Isotype (clone MOPC-173, purple histogram). Non-specific binding of the same antibody on untreated cells is shown in red.
  • Cell-Vive_CD_Human_TruStain_FcX_GMP_083022
    THP-1 cells were treated with Cell-Vive™ Ultra-LEAF™ Defined Human TruStain FcX™, GMP followed by staining with an irrelevant PE mouse IgG2a, κ Isotype (clone MOPC-173, purple histogram). Non-specific binding of the same antibody on untreated cells is shown in red.
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420510 100 tests 220€
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Description

Human Fc receptors (FcRs) are molecules that regulate the immune responses. FcRs are expressed by several hematopoietic cells. Due to FcRs-mediated Ig Fc binding, false positive or false negative results of immunofluorescent staining could occur. Cell-Vive™ Ultra-LEAF™ Defined Human TruStain FcX™, GMP is specially formulated to block the FcR-involved unwanted staining without interfering with the antibody-mediated specific staining of human cells. Cell-Vive™ Ultra-LEAF™ Defined Human TruStain FcX™, GMP is compatible with flow cytometric staining with anti-human CD16 (clone 3G8), CD32 (clone FUN-2), and CD64 (clone 10.1) antibodies. Cell-Vive™ Ultra-LEAF™ Defined Human TruStain FcX™, GMP contains ultra-low endotoxin levels (<0.01EU/µg of protein) and is tested negative for mycoplasma and microbial growths.
This GMP product is for research and further ex vivo bioprocessing use only.

Product Details
Quality Statement

BioLegend Cell-Vive™ GMP cell culture products are manufactured and tested in accordance with USP Chapter 1043, Ancillary Materials for Cell, Gene and Tissue-Engineered Products and Ph. Eur. Chapter 5.2.12 in a dedicated GMP facility compliant with ISO 13485:2016. Specifications and processes include

  • Low endotoxin level (< 1 EU/mL)
  • Mycoplasma and bacterial/fungal growth testing
  • Batch-to-batch consistency
  • Vendor qualification
  • Raw material traceability and documentation
  • Documented procedures and employee training
  • Equipment maintenance and monitoring records
  • Lot-specific certificates of analysis
  • QA review of released products
  • Quality audits per ISO 13485:2016
Technical Data Sheet (pdf)

Product Details

Formulation
Serum-Free, Defined, containing no preservatives. Endotoxin level is < 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
Endotoxin Level
< 1 EU/mL
Storage & Handling
Store between 2°- 8°C. Handle under aseptic conditions.
Application

FC - Quality tested
Recommended Usage

5 μl of Cell-Vive™ Ultra-LEAF™ Defined Human TruStain FcX™, GMP per million cells in 100 μl staining buffer.

Application Notes

This buffer contains specialized human IgG. It is not recommended to be used for staining human IgG. Handle as biohazard agent. Add 5 μl of Cell-Vive™ Ultra-LEAF™ Defined Human TruStain FcX™, GMP per million cells in 100 μl staining buffer, mix and incubate at room temperature for 5-10 minutes prior to staining with antibody of interest. It is not necessary to wash cells between these blocking and immunostaining steps.

Disclaimer

BioLegend Cell-Vive™ GMP Cell Culture products are for research use only. Suitable for ex vivo cell processing. Not for injection or diagnostic or therapeutic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

Antigen Details

Antigen References
  1. Bruhns P, 2012. Blood. 119:5640-9
  2. Patera A, et al. 2016. J Leukoc Biol. 100:1239-1254. PubMed
  3. Kreutmair S, et al. 2021. Immunity. 54:1578-1593. PubMed
Gene ID
NA
Go To Top Version: 5    Revision Date: 08.01.2024

For Research Use Only. Suitable for ex vivo cell processing. Not for injection or diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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