Cell-Vive™ GMP Recombinant Human FGF-4 (carrier-free)

Pricing & Availability
Other Names
Fibroblast Growth Factor 4 (FGF-4), Heparin-Binding Growth Factor 4 (HBGF-4), HST-1, K-FGF, KS3, Kaposi Sacrcoma Oncogene, Heparin Secretory Transforming Protein 1 (HSTF-1)
Ave. Rating
Submit a Review
Product Citations
publications
CV_GMP_RECOM_Human_FGF-4_083024
NIH3T3 proliferation induced by human FGF-4.
  • CV_GMP_RECOM_Human_FGF-4_083024
    NIH3T3 proliferation induced by human FGF-4.
Cat # Size Price Quantity Check Availability Save
592226 500 µg 2792€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
592224 50 µg 716€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

FGF-4 was first identified as HST-1 gene by a NIH3T3 transforming assay. It belongs to the FGF family that includes 22 members FGF1-FGF23. FGF-15 has not been identified in humans. FGF-4 exhibits strong effects on many different cell types and tissues, and plays important roles in both embryogenesis and adult tissue stem cell development in a variety of organisms. FGF-4 can stimulate limb mesenchyme proliferation and can provide all the signals required for normal outgrowth and patterning of the limb. FGF-4 mRNA has been detected in the apical ectodermal ridge of the limb bud in Days 11 and 12 embryos. FGF-4 is also involved in early heart development by supporting the proliferation and differentiation of precardiac myoblasts. In adults, FGF-4 is expressed in the testis and its overexpression results in enhanced spermatogenesis. FGF-4 is also a potent inducer of platelet production from megakaryocytes. FGF-4 expression has been detected in the brain at both neonatal and adult stages. FGF-4 can induce neural stem cell proliferation and neuronal differentiation. The diverse functions of FGF-4 are mediated by FGF receptors (FGFR) that contain an extracellular ligand-binding domain with three immunoglobulin-like domains. Like other FGFs, FGF-4 also binds to anionic glycosaminoglycans heparin and heparin sulfate with high affinity. Heparin sulfate availability has been shown to regulate the binding between FGF-4 and its receptors. The role of FGF-4 in cancer has also been extensively investigated. FGF-4 is an angiogenic protein, and amplification of FGF-4 gene has been found in many human tumors. It has been shown that FGF-4 is a potential target for the treatment of human testicular tumors.

Product Details
Quality Statement

BioLegend Cell-Vive™ GMP Recombinant proteins are manufactured and tested in accordance with USP Chapter 1043, Ancillary Materials for Cell, Gene and Tissue-Engineered Products and Ph. Eur. Chapter 5.2.12 in a dedicated GMP facility compliant with ISO 13485:2016. Specifications and processes include:

  • Low endotoxin level (≤ 0.1 EU/μg)
  • Purity (≥ 95% or higher)
  • Bioburden testing
  • Mycoplasma testing
  • Batch-to-batch consistency
  • Vendor qualification
  • Raw material traceability and documentation
  • Documented procedures and employee training
  • Equipment maintenance and monitoring records
  • Lot-specific certificates of analysis
  • Quality audits per ISO 13485:2016
  • QA review of released products
Technical Data Sheet (pdf)

Product Details

Source
Human FGF-4, amino acids Ser54-Leu206 (Accession# P08620) was expressed in E. coli.
Molecular Mass
The 153 amino acid recombinant protein has a predicted molecular mass of approximately 19.7 kD. The non-reduced and DTT-reduced protein migrates at approximately 19.7 kD by SDS-PAGE. The predicted N-terminal amino acid is Serine.
N-terminal
Sequence Analysis
Ser-Leu-Ala-Arg-Leu-Pro-Val-Ala-Ala-Gln
Purity
≥ 95%, as determined by Coomassie stained SDS-PAGE
Formulation
Protein was lyophilized from 0.1 μm filtered solution containing PBS.
Endotoxin Level
Less than or equal to 0.1 EU per μg of protein as determined by LAL method.
Residual Host Cell Protein Content
≤ 0.500 ng/μg by ELISA
Concentration
50 μg and 500 μg sizes are lyophilized
Storage & Handling
Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at - 20°C or colder until the expiration date. Reconstitute lyophilized protein in sterile PBS. Before reconstitution, make sure sterile PBS and product are at room temperature. Quickly spin the vial or gently tap down on the vial to make sure the lyophilized product is at the bottom of the vial before opening. Use aseptic techniques to add the required volume of reconstitution buffer (sterile PBS) to the vial, to obtain the recommended stock concentration 250 μg/mL. Close the vial and leave at ambient temperature for 15-20 minutes. Then gently invert the vial several times or until all of the lyophilized product dissolves. Leave the vial at room temperature for another 15 minutes. If small particulates are still observed after 15 minutes, incubate at room temperature for an additional 30 minutes and leave the vial at 2°C - 8°C overnight. Next day, invert the vial several times or gently pipette the solution up and down before use. If needed, transfer the reconstituted stock solution to a sterile container for additional dilution to no less than 100 μg/mL. Small working aliquots in polypropylene tubes can be made after reconstitution and store the vials at -20°C or lower. Avoid freeze/ thaw cycles. Carrier protein such as 0.2 - 1% endotoxin-free BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to two weeks or stored at -20°C or colder for up to 3 months.
Activity
The ED50 is 0.2-1 ng/mL as determined by a dose-dependent stimulation of NIH3T3 cell proliferation.
Application

Bioassay

Application Notes

Our lyophilized proteins are validated in-house to maintain activity after shipping at ambient temperature and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.

Application References
  1. Qi S, et al. 2019. Small. 15:e1804332. PubMed
Disclaimer

BioLegend Cell-Vive™ GMP Recombinant proteins are for research use only. Suitable for ex vivo cell processing. Not for injection or diagnostic or therapeutic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

Antigen Details

Structure
Growth factor
Distribution

Expression of FGF-4 is highly restricted to undifferentiated stem cells.

Function
FGF-4 is a mitogenic, angiogenic, and survival factor. It is involved in cell proliferation and differentiation in embryonic development processes. N-glycosylation may negatively regulate FGF-4 activity.
Interaction
Precardiac myoblasts, megacariocytes, neural stem cells.
Ligand/Receptor
FGFR-I, IIIc; FGFR-2, IIIc; FGFR-3, IIIc, FGFR-4.
Bioactivity
Human FGF-4 induces the proliferation of NIH3T3 cells.
Antigen References
  1. Konishi H, et al. 1996. Oncogene. 13:9.
  2. Sun X, et al. 2000. Nat Genet. 25:83-6.
  3. Yamamoto H, et al. 2002. Oncogene. 21:899-908.
  4. Kosaka N, et al. 2006. FASEB J. 20:1484-5.
  5. Kosaka N, et al. 2009. Dev. Dyn. 238:265-76.
  6. Arao T, et al. 2013. Hepatology. 57:1407-15.
  7. Itoh N and Ohta H. 2013. Front. Physiol. 4:247.
Gene ID
2249 View all products for this Gene ID
UniProt
View information about FGF-4 on UniProt.org
Go To Top Version: 1    Revision Date: 09.12.2024

For Research Use Only. Suitable for ex vivo cell processing. Not for injection or diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

ProductsHere

Login / Register
Remember me
Forgot your password? Reset password?
Create an Account