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- Regulatory Status
- RUO
- Other Names
- SCI, LD78, MIP1A, SCYA3, G0S19-1, LD78ALPHA, MIP-1-alpha
- Ave. Rating
- Submit a Review
- Product Citations
- publications
| Cat # | Size | Price | Quantity Check Availability | Save | ||
|---|---|---|---|---|---|---|
| 479904 | 5 plates | 280€ | ||||
MIP-1α, also known as CCL3, is a member of the CC subfamily of chemokines, a group of chemoattractant cytokines that play a crucial role in recruiting leukocytes to sites of tissue injury or inflammation. This multifunctional peptide is secreted by a variety of both hematopoietic and non-hematopoietic cells, including monocytes, T and B lymphocytes, neutrophils, dendritic cells, and natural killer cells. Notably, MIP-1α/CCL3 can bind to multiple chemokine receptors, particularly CCR1 and CCR5, through which it recruits macrophages, lymphocytes, and eosinophils. It is commonly found in areas of inflammation and bone resorption, where it attracts activated CD8+ T cells and promotes osteoclastogenesis. The synthesis of MIP-1α/CCL3 remains at low levels under normal physiological conditions but can be rapidly upregulated upon stimulation by endotoxins like lipopolysaccharide (LPS), viral proteins, or pro-inflammatory cytokines such as IL-1β. This chemokine plays a key pathological role in various inflammatory and autoimmune diseases, including multiple myeloma, rheumatoid arthritis, Sjögren syndrome, sarcoidosis, respiratory disease, cardiovascular disease, and periodontitis.
Product DetailsKit Contents
- Kit Contents
-
- Human CCL3 ELISA MAX™ Capture Antibody (200X)
- Human CCL3 ELISA MAX™ Detection Antibody (200X)
- Human CCL3 Lyophilized Standard
- Avidin-HRP (1000X)
- Substrate Solution D
- Coating Buffer B (5X)
- Assay Diluent A (5X)
Product Details
- Verified Reactivity
- Human
- Application
-
ELISA
- As a part of BioLegend's efforts to go green, each new lot of the ELISA MAX™ sets will include a summarized version of its manual. Click here for more information.
- Sensitivity
- 11.0 pg/mL
- Standard Range
- 31.3-2000 pg/mL
- Materials Not Included
-
- Microwell plates: BioLegend Cat. No. 423501 is recommended
- Wash Buffer: BioLegend Cat. No. 421601 is recommended, or PBS + 0.05% Tween-20
- Stop Solution: BioLegend Cat. No. 423001 is recommended, or acid solution, e.g. 2N H2SO4
- Plate Sealers: BioLegend Cat. No. 423601 is recommended
- PBS (Phosphate-Buffered Saline): 8.0 g NaCl, 1.16 g Na2HPO4, 0.2 g KH2PO4, 0.2 g KCl, add deionized water to 1 L; pH to 7.4, 0.2 μm filtered
- Deionized (DI) water
- A microplate reader capable of measuring absorbance at 450 nm
- Adjustable pipettes to measure volumes ranging from 2 μL to 1 mL
- Wash bottle or automated microplate washer
- Log-Log graph paper or software for data analysis
- Tubes to prepare standard dilutions
- Timer
Antigen Details
- Cell Type
- Hematopoietic stem and progenitors
- Biology Area
- Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience, Stem Cells
- Molecular Family
- Cytokines/Chemokines
- Gene ID
- NA
- UniProt
- View information about CCL3 on UniProt.org
Related FAQs
- Do the ELISA MAX™ Deluxe Sets come with plates?
-
No, ELISA MAX™ Deluxe Sets do not come with plates. Coating Buffer and Assay Diluent (for blocking and dilutions) are included in these sets. Plates can be ordered separately (Cat No. 423501), and instructions for coating the plates are in the sets’ product manuals.
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