- Regulatory Status
- RUO
- Other Names
- Interferon gamma, IFN-gamma
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Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
430124 | 5 plates | 492€ |
Human IFN-γ (Interferon-gamma) is a multifunctional cytokine which is the sole member of the class II Interferon. Although a variety of immune cells can express IFN-γ, most of IFN-γ is produced by T-cells and NK cells. It has critical roles in many processes including, but not limited to, anti-viral response, regulating Th1/Th2 balance, enhancing antigen presentation, and controlling cellular proliferation and apoptosis. IFN-γ can bind to 2 receptors: IFNGR1 and IFNGR2, which transduce the signal downstream through the JAK-STAT pathway. Because of its multifunction nature, dysregulation of IFN-γ expression and activity has been implicated in many diseases such as autoimmune and cancer.
BioLegend’s ELISpot MAX™ Deluxe Set contains pre-optimized essential reagents and additional buffers and solution for Enzyme-Linked Immunosorbent Spot assay. This kit is designed for detection of human IFN-γ secreting cells. A monoclonal antibody specific for human IFN-γ is coated on ethanol-activated 96-well PVDF membrane plate as the capture. Stimulated and/or unstimulated cells are pipetted directly into the wells, and then incubated at 37°C for a specific period of time. During this period, capture antibody binds to human IFN-γ from the secreting cells in the vicinity. Then, cells are removed, and a biotinylated anti-human IFN-γ detection antibody is added, followed by Avidin-HRP. Subsequently, TMB membrane substrate is added. The TMB membrane substrate reacts with HRP to form a blue-black precipitate at the sites of human IFN-γ secreting cells. The end result is visible spots, which correspond to footprints of IFN-γ secreting cells on the membrane surface.
Kit Contents
- Kit Contents
-
- Human IFN-γ ELISpot MAX™ Capture Antibody (200X)
- Human IFN-γ ELISpot MAX™ Detection Antibody (200X)
- Human IFN-γ Standard (used as positive control)
- Avidin-HRP (1000X)
- TMB Membrane Substrate
- Coating Buffer A (5X)
- Assay Diluent B (5X)
Product Details
- Verified Reactivity
- Human
- Application
-
ELISpot
- Materials Not Included
-
- PVDF-bottom plate: Millipore MultiScreenHTS IP Filter Plate (Cat. No. MSIPS4W10) is recommended.
- Wash Buffer: BioLegend Cat. No. 421601 is recommended, or PBS + 0.05% Tween-20
- Plate Sealers: BioLegend Cat. No. 423601 is recommended
- 0.22 μm filters
- 35% Ethanol
- Sterile PBS (Phosphate-Buffered Saline): 8.0 g NaCl, 1.16 g Na2HPO4, 0.2 g KH2PO4, 0.2 g KCl, add deionized water to 1 L; pH to 7.4, 0.22 μm filtered
- Sterile cell culture media
- 37°C CO2 incubator
- Deionized (DI) water
- Adjustable pipettes to measure volumes ranging from 2 μL to 1 mL
- Wash bottle, manifold dispenser, or automated microplate washer
- Tubes for dilution
- Timer
- Stereomicroscope or an automated ELISpot reader
Antigen Details
- Cell Type
- NK cells, T cells
- Biology Area
- Immuno-Oncology, Immunology, Innate Immunity, Neuroscience, Signal Transduction
- Molecular Family
- Cytokines/Chemokines, Innate Immune Signaling, Tumor Suppressors
- Gene ID
- 3458 View all products for this Gene ID
- UniProt
- View information about IFN-gamma on UniProt.org
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