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FITC anti-human CD73 (Ecto-5'-nucleotidase) Antibody

Pricing & Availability
Clone
AD2 (See other available formats)
Regulatory Status
RUO
Workshop
V B-CD73.3
Other Names
Ecto-5'-nucleotidase, E.C3.1.3.5, L-VAP-2, NT5E, 5'-NT
Isotype
Mouse IgG1, κ
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Product Citations
publications
A_AD2_FITC_CD73_Antibody_FC_1_020713
Human peripheral blood lymphocytes were stained with CD3 APC and CD73 (clone AD2) FITC (top) or mouse IgG1, κ FITC isotype control (bottom).
  • A_AD2_FITC_CD73_Antibody_FC_1_020713
    Human peripheral blood lymphocytes were stained with CD3 APC and CD73 (clone AD2) FITC (top) or mouse IgG1, κ FITC isotype control (bottom).
  • B_AD2_FITC_CD73_Antibody_FC_2_020713
Compare all formats See FITC spectral data
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344015 25 tests 90€
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344016 100 tests 173€
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Description

CD73 is a 70 kD glycophosphatidylinositol (GPI)-linked 5'-nucleotidase, which is also known as ecto-5'-nucleotidase. It converts adenosine monophosphate (AMP) to adenosine. CD73 is expressed on subsets of T and B cells, mesenchymal stem cells, follicular dendritic cells, endothelial cells, and epithelial cells. It has been reported that CD73 costimulates T cell activation, and mediates adhesion of lymphocytes to follicular dendritic cells and endothelial cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Reported Reactivity
African Green, Baboon
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with FITC under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Application Notes

Additional reported applications (for the relevant formats) include:immunofluorescence3.

Clone AD2 has been noted to induce clustering and internalization of CD73 in vivo and inhibit metastasis in a murine breast cancer xenograft model4.

Application References
  1. Nakamura T, et al. 1993. J. Immunol. 151:6933.
  2. Liao J, et al. 2011. J Endod. 37:1217. PubMed
  3. Touboul C, et al. 2013. J. Transl. Med. 11:28. (IF)
  4. Terp MG, et al. 2013. J Immunol. 191: 4165-73 (Block)
Product Citations
  1. Huang J, et al. 2022. Clin Exp Pharmacol Physiol. 49:881. PubMed
  2. Zhang T, et al. 2022. IUBMB Life. 74:1081. PubMed
  3. Xia KS, et al. 2023. Bioact Mater. 21:69. PubMed
  4. Wang H, et al. 2022. Oxid Med Cell Longev. 2022:3082969. PubMed
  5. Tevlin R, et al. 2023. Sci Rep. 13:2097. PubMed
  6. Aru B, et al. 2023. Balkan Med J. 40:117. PubMed
  7. Chen X, et al. 2023. Exp Ther Med. 25:99. PubMed
  8. Yusa K, et al. 2016. Sci Rep. 6:29462. PubMed
  9. Lee CW, et al. 2022. J Adv Res. 39:73. PubMed
  10. Bowley TY, et al. 2022. Cancer Res Commun. 2:1436. PubMed
  11. Lu Y, et al. 2023. Sci Rep. 13:8479. PubMed
  12. Hoover MY, et al. 2023. Nat Protoc. . PubMed
  13. Goodnough LH, et al. 2020. JBMR Plus. 4:e10398. PubMed
  14. Liu X, et al. 2020. Exp Ther Med. 1.258333333. PubMed
  15. Tu S, et al. 2020. Cellular Signalling. 73:109695. PubMed
  16. Vishnoi M, et al. 2018. Cancer Res. 78:5349. PubMed
  17. Han J, et al. 2022. FEBS J. 289:417. PubMed
  18. Yerneni SS, et al. 2021. MBio. 12:e0165721. PubMed
  19. Morad G, et al. 2020. International Journal of Molecular Sciences. 21(11):3851. PubMed
  20. Sun W, et al. 2019. Exp Ther Med. 17:2199. PubMed
  21. Liu Y, et al. 2022. iScience. 25:104405. PubMed
  22. Yan K, et al. 2022. iScience. 25:104822. PubMed
  23. Gao P, et al. 2017. Cytotechnology. 69:751. PubMed
  24. Yu C, et al. 2020. Sci Rep. 10:14521. PubMed
  25. Ambrosi TH, et al. 2020. Aging Cell. 19:e13164. PubMed
  26. Ayhan S, et al. 2021. Journal of Cell Science. 134(6):. PubMed
  27. Zhang Q, et al. 2021. Bone Joint Res. 10:226. PubMed
  28. Keshtkar S, et al. 2021. Stem Cells Int. 8857457:2020. PubMed
  29. Ludwig N, et al. 2020. Angiogenesis. 1.374305556. PubMed
  30. Aomatsu E, et al. 2014. Sci Rep. 4:3652. PubMed
  31. He Z, et al. 2020. J Exp Clin Cancer Res. 39:140. PubMed
  32. Fei D, et al. 2021. Front Cell Dev Biol. 9:687258. PubMed
  33. Pauken CM, et al. 2021. Cancers (Basel). 13:. PubMed
  34. Festag J, et al. 2020. Mol Ther Nucleic Acids. 1.330555556. PubMed
  35. Vishnoi M, et al. 2019. Mol Oncol. 13(9): 1913. PubMed
  36. Boucher JM, et al. 2018. Cardiovasc Drugs Ther. 32:519. PubMed
  37. Zheng C, et al. 2021. J Extracell Vesicles. 10:e12109. PubMed
  38. Sprouse ML, et al. 2019. Int J Mol Sci. 20:8. PubMed
RRID
AB_2561808 (BioLegend Cat. No. 344015)
AB_2561808 (BioLegend Cat. No. 344016)

Antigen Details

Structure
GPI-linked 5'-nucleotidase, 70 kD
Distribution

Subsets of T cells and B cells, mesenchymal stem cells, follicular dendritic cells, endothelial cells, and epithelial cells

Function
Catalyses dephosphorylation of adenosine monophosphate, costimulates T cell activation, mediates adhesion of lymphocytes to follicular dendritic cells and endothelial cells
Cell Type
B cells, Dendritic cells, Endothelial cells, Epithelial cells, Mesenchymal Stem Cells, T cells, Tregs
Biology Area
Costimulatory Molecules, Immunology, Stem Cells
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Zola H, et al. 2007. Leukocyte and stromal Cell Molecules:the CD Markers. A John Wiley & Sons Inc, Publication.
2. Airas L and Jalkanen  S, et al. 1996. Blood 88:1755.
3. Gutensohn W, et al. 1995. Cell Immunol. 161:213.
4. Airas L, et al. 1995. J. Exp. Med. 182:1603.

Gene ID
4907 View all products for this Gene ID
UniProt
View information about CD73 on UniProt.org

Other Formats

View All CD73 Reagents Request Custom Conjugation
Description Clone Applications
FITC anti-human CD73 (Ecto-5'-nucleotidase) AD2 FC
Brilliant Violet 421™ anti-human CD73 (Ecto-5'-nucleotidase) AD2 FC,IHC-F
Purified anti-human CD73 (Ecto-5'-nucleotidase) AD2 FC,IHC-F,Block
PE anti-human CD73 (Ecto-5'-nucleotidase) AD2 FC
APC anti-human CD73 (Ecto-5'-nucleotidase) AD2 FC
PE/Cyanine7 anti-human CD73 (Ecto-5'-nucleotidase) AD2 FC
Pacific Blue™ anti-human CD73 (Ecto-5'-nucleotidase) AD2 FC
PerCP/Cyanine5.5 anti-human CD73 (Ecto-5'-nucleotidase) AD2 FC
Biotin anti-human CD73 (Ecto-5'-nucleotidase) AD2 FC
PE/Dazzle™ 594 anti-human CD73 (Ecto-5'-nucleotidase) AD2 FC
APC/Cyanine7 anti-human CD73 (Ecto-5'-nucleotidase) AD2 FC
Brilliant Violet 605™ anti-human CD73 (Ecto-5'-nucleotidase) AD2 FC
Brilliant Violet 711™ anti-human CD73 (Ecto-5'-nucleotidase) AD2 FC
Brilliant Violet 785™ anti-human CD73 (Ecto-5'-nucleotidase) AD2 FC
TotalSeq™-A0577 anti-human CD73 (Ecto-5'-nucleotidase) AD2 PG
TotalSeq™-C0577 anti-human CD73 (Ecto-5'-nucleotidase) AD2 PG
TotalSeq™-B0577 anti-human CD73 (Ecto-5'-nucleotidase) AD2 PG
APC/Fire™ 750 anti-human CD73 (Ecto-5'-nucleotidase) AD2 FC
TotalSeq™-D0577 anti-human CD73 (Ecto-5'-nucleotidase) AD2 PG
Alexa Fluor® 700 anti-human CD73 (Ecto-5'-nucleotidase) AD2 FC
Alexa Fluor® 647 anti-human CD73 (Ecto-5'-nucleotidase) AD2 FC
Brilliant Violet 510™ anti-human CD73 (Ecto-5'-nucleotidase) AD2 FC
PerCP/Fire™ 780 anti-human CD73 (Ecto-5'-nucleotidase) AD2 FC
Spark Red™ 718 anti-hu CD73 (Ecto-5'-nucleotidase) (Flexi-Fluor™) AD2 FC
PerCP/Fire™ 806 anti-human CD73 (Ecto-5'-nucleotidase) AD2 FC
Spark PLUS B550™ anti-human CD73 (Ecto-5'-nucleotidase) AD2 FC
Go To Top Version: 2    Revision Date: 04.09.2014

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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