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LEGEND MAX™ Mouse IL-25 (IL-17E) ELISA Kit

Pricing & Availability
Regulatory Status
RUO
Other Names
IL25, IL-17E, IL17E, Interleukin 25, Interleukin 17E
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Product Citations
publications
LEGEND-MAX_Mouse_IL-25_1_052819
  • LEGEND-MAX_Mouse_IL-25_1_052819
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447107 1 Pre-coated Plate 370€
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Description

IL-25, also known as IL-17E, is a pro-inflammatory cytokine encoded by the IL25 gene located on chromosome 14. The IL-17 family consists of IL-17A, IL-17B, IL-17C, IL-17F, IL-17A/F, and IL-17E. The IL-17 family has a similar protein structure with four conserved cysteine residues and two disulfide bonds. IL-25 expression is widely distributed and can be produced by a variety of cells and tissues. IL-25 induces T helper 2 (Th2) expansion and production of cytokines IL-4, IL-5, and IL-13. This is important in eosinophil expansion, regulating gut immunity, and in the response to helminth parasites.

IL-25 has been demonstrated to play a role in allergy and asthma. IL-25 levels are positively correlated with the severity of allergenic rhinitis in patients. Blocking IL-25 has been proposed as an allergy treatment in combination with other therapies. IL-25 has also been associated with the pathogenesis of rheumatoid arthritis and is frequently upregulated in the serum and synovial fluid of patients and in mouse models of rheumatoid arthritis.

BioLegend’s LEGEND MAX™ Mouse IL-25 (IL-17E) ELISA kit is a Sandwich Enzyme-Linked Immunosorbent Assay (ELISA) with a 96-well strip plate. The plate is pre-coated with a monoclonal rat capture antibody. The detection antibody is a biotinylated rat monoclonal antibody. This kit is specifically designed for the accurate quantitation of mouse IL-25 from cell culture supernatant, serum, plasma and other biological fluids. It is analytically validated with ready-to-use reagents.

Product Details
Technical Data Sheet (pdf)

Kit Contents

Kit Contents
  • Mouse IL-25 (IL-17E) Pre-coated 96-well Strip Microplate
  • Mouse IL-25 (IL-17E) Dectection Antibody
  • Mouse IL-25 (IL-17E) Standard
  • Avidin-HRP A
  • Assay Diluent D
  • Assay Buffer B
  • Wash Buffer (20X)
  • Substrate Solution F
  • Stop Solution
  • Plate Sealers

Product Details

Verified Reactivity
Mouse
Application

ELISA
Product Citations
  1. Zhang J, et al. 2019. Stem Cells. 1319:37. PubMed
  2. Wiesner DL, et al. 2020. Cell Host Microbe. 614:27. PubMed
Sensitivity
2.681 + 0.459 pg/mL
Standard Range
1.6 - 0.025 ng/mL
Materials Not Included
  • Microplate reader able to measure absorbance at 450 nm
  • Adjustable pipettes to measure volumes ranging from 1 µL to 1,000 µL
  • Deionized water
  • Wash bottle or automated microplate washer
  • Log-Log graph paper or software for data analysis
  • Polypropylene tubes to prepare standard dilutions
  • Timer
  • Plate Shaker
  • Polypropylene vials

Antigen Details

Cell Type
Basophils, Dendritic cells, Eosinophils, Epithelial cells, Macrophages, T cells
Biology Area
Immuno-Oncology, Immunology
Molecular Family
Cytokines/Chemokines
Gene ID
NA
UniProt
View information about IL-25 on UniProt.org

Related FAQs

In your LEGEND MAX™ ELISA Kits, there is a step that calls for washing the plates before adding sample. What is the purpose of this step?

We typically use a stabilizer for pre-coated plates. The additional washing step is designed to remove these components before you start the assay. If you do not perform the washing, the effect on assay performance is negligible.

I have multiple LEGEND MAX™ ELISA kits that I want to run simultaneously. Can I use the same wash buffer for all the kits?

The wash buffer provided in all our LEGEND MAX™ kits is the same and the part numbers on the wash buffer bottles in these kits should be identical. For ELISA MAX™ Deluxe and ELISA MAX™ Standard Sets, we provide a recipe for the wash buffer on each kit’s technical data sheet. This recipe is the same for all ELISA MAX™ sets.

For some of your ELISA kits, why do my serum samples require dilution with assay buffer?

In some cases, dilution with assay buffer is required to minimize the matrix difference between the samples and the standards to achieve better accuracy.

Go To Top Version: 2    Revision Date: 11.16.2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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