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LEGENDplex™ COVID-19 Cytokine Storm Panel 2 (13-plex) w/ VbP

Pricing & Availability
Regulatory Status
RUO
Other Names
IL-13, GM-CSF, IL-1β, IL-5, sCD25 (IL-2Ra), IL-4, VEGF, IL-17A, IL-18, APRIL, CCL4 (MIP-1β), IL-15, IL-12p70
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Cat # Size Price Quantity Save
741112 100 tests 1503€
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Description

Coronavirus disease 2019 (COVID-19), caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), has spread rapidly across the world to become a global pandemic. Although most patients have a favorable prognosis and recover; those with severe illness may develop dyspnea and hypoxemia which can quickly progress to or death. Accumulating clinical evidence has shown an abnormal and uncontrolled production of cytokines, termed a cytokine storm, in critically ill COVID-19 patients. This cytokine storm is the major contributing factor in the clinical severity of the disease. As shown by multiple scientific reports, COVID-19 cytokine storm and subsequent ARDS is driven by the fulminant effects of many immune effector molecules including chemokines, interferons, interleukins, colony-stimulating factors and TNF-α.

The COVID-19 Cytokine Storm Panel 2 is a multiplex bead-based assay panel, using fluorescence–encoded beads suitable for use on various flow cytometers. This panel allows simultaneous quantification of 13 human cytokines, including IL-13, GM-CSF, IL-1β, IL-5, sCD25 (IL-2Ra), IL-4, VEGF, IL-17A, IL-18, APRIL, MIP-1β (CCL4), IL-15, and IL-12p70. This assay panel provides higher detection sensitivities and broader dynamic ranges than traditional ELISA methods. The panel has been validated by detecting expected changes in biological samples.

The LEGENDplex™ Cytokine Storm Panel 2 (13-plex) is designed to allow flexible customization within the panel. It can also be divided into subpanels. The panel has been validated for use on cell culture supernatant, serum, and plasma samples.
 

Cat #

Plex Size

Targets

Sample Type

Dilution Factor

741111, 741112

13-plex

IL-13, GM-CSF, IL-1β, IL-5, sCD25 (IL-2Ra), IL-4, VEGF, IL-17A, IL18, APRIL, CCL4, IL-15, IL-12p70

Tissue Culture

Varies

741141, 741142

12-plex

IL-13, GM-CSF, IL-1β, IL-5, sCD25 (IL-2Ra), IL4, VEGF, IL-17A, IL-18, CCL4, IL-15, IL-12p70

Tissue Culture

Varies

Serum/Plasma

2X

741143, 741144

1-plex

APRIL

Tissue Culture

Varies

Serum

200X
Plasma 10X


Please visit www.biolegend.com/legendplex for more information on how to mix and match within the panel.

Product Details
View Current Manual (pdf)

Kit Contents

Kit Contents
  • Setup Beads: PE Beads 
  • Setup Beads: Raw Beads 
  • Capture beads 
  • COVID-19 Cytokine Storm Panel 1 & 2 Detection Antibodies 
  • COVID-19 Cytokine Storm Panel 1 & 2 Standard Cocktail, Lyophilized 
  • LEGENDplex™ SA-PE 
  • LEGENDplex™ Matrix B2, Lyophilized
  • LEGENDplex™ Assay Buffer 
  • LEGENDplex™ Wash Buffer, 20X 
  • Plate Sealers 
  • V-bottom Plate

Product Details

Verified Reactivity
Human
Application

Multiplex
Learn more about LEGENDplex™ at biolegend.com/legendplex

Download the LEGENDplex™ software here.
Materials Not Included
  • Flow Cytometer
  • Pipettes and Tips
  • Reagent Reservoirs for Multichannel Pipettes
  • Polypropylene Microfuge Tubes
  • Vortex Mixer
  • Sonicator
  • Aluminum Foil
  • Absorbent Pads or Paper Towels
  • Plate Shaker
  • Tabletop Centrifuges
  • A Vacuum Filtration Unit and a Vacuum Source (if using filter plates)
  • Centrifuge with a swinging bucket adaptor for microtiter plates (if using V-bottom plates)
  • 1.1 mL polypropylene micro FACS tubes, in 96-tube rack

Related FAQs

If I don't have a vacuum, how do I remove the liquid from my plate?

If you do not have a vacuum, the assay should be run in a V-bottom plate. After centrifugation using a swinging-bucket rotor with a plate adaptor, you can remove the liquid by flicking the plate quickly, dumping the contents into a sink, and patting it dry carefully on a stack of clean paper towels without losing the beads. Alternatively, you can remove the liquid by using a pipette.

Should I perform the assay with the filter plates or with V-bottom plates?

Filter plates or V-bottom plates have been included in some kits for your convenience. A vacuum filtration unit is required to work with the filter plates. However, if you don’t have access to a vacuum manifold or if you prefer, then you can use the V-bottom plates and follow the recommended assay protocols for the type of plates you choose. All plates should be made from low binding polypropylene. Polystyrene ELISA or cell culture plates should not be used.

After I finish the staining process, how long can I wait before reading my LEGENDplex™ samples?

The samples can be kept overnight at 4°C while being protected from exposure to light and be read the next day. There may be a decrease in signal, but overall, the assay results should not be affected. Storing the samples for extended periods of time is not recommended, as it could lead to further reductions in signal.

What is the shelf life of LEGENDplex™ kits?

LEGENDplex™ kits are guaranteed for 6 months from the date of receipt, but may have a shelf life of up to 2 years from the date of manufacture.

Is special software required for data analysis?

Typically flow cytometers generate output files in FCS format (e.g. FCS 2.0, 3.0, or 3.1) and in some cases in list mode file format (LMD). Other software may be available to analyze FCS files. Data generated using LEGENDplex™ kits can be analyzed using the freely available LEGENDplex™ data analysis software. Please check our website for the most updated versions of the software.

Go To Top Version: 4    Revision Date: 06.15.2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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