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A single cell suspension from human PBMCs was prepared for the positive selection of CD56+ cells using the MojoSort™ Human CD56 Nanobeads. The cells were incubated with True-Stain Monocyte Blocker™ prior to the addition of the Human CD56 Nanobeads. After separation, the cells were stained with anti-human CD56 (NCAM) (clones 5.1H11 and HCD56) PE and anti-human CD3 (clone UCHT1) APC. Dead cells were excluded by Helix NP™ Blue. Purity of the CD56+ cells ranges between 80-90% due to donor variation. -
A single cell suspension from human PBMCs was prepared for the positive selection of CD56+ cells using the MojoSort™ Human CD56 Nanobeads. The cells were incubated with True-Stain Monocyte Blocker™ prior to the addition of the Human CD56 Nanobeads. After separation, the cells were stained with anti-human CD56 (NCAM) (clones 5.1H11 and HCD56) PE and anti-human CD335 (NKp46) (clone 9E2) APC. Dead cells were excluded by Helix NP™ Blue.
| Cat # | Size | Price | Quantity Check Availability | Save | ||
|---|---|---|---|---|---|---|
| 480155 | 10 tests | 239€ | ||||
| 480156 | 100 tests | 698€ | ||||
Human CD56+ cells are either selected or depleted by incubating the sample with the directly conjugated CD56 Nanobeads. The magnetically labeled fraction is retained by the use of a magnetic separator. After collection of the CD56+ cells, downstream applications include functional assays, gene expression, phenotypic characterization, etc.
MojoSort™ reagents are also compatible with column-based cell separation systems available from other vendors. Optimized protocols for cell separation using columns from in-house testing are provided for each kit under the “Related Protocols” section, as well as representative data on the product webpage (where available). Data generated using column separators are indicated on the figure legend.
Due to the property of the beads, MojoSort™ reagents typically require dilution for optimal use on column separators. Where available, recommended dilution factors for each kit component based on in-house testing are provided under the “Application Notes” section of the webpage.
Kit Contents
- Kit Contents
-
For Cat. No. 480155:
• 1 vial containing 200 μL of True-Stain Monocyte Blocker™
• 1 vial containing 100 µL of Human CD56 Nanobeads
For Cat. No. 480156:
• 2 vials containing 1 mL of True-Stain Monocyte Blocker™
• 1 vial containing 1 mL of Human CD56 Nanobead
Product Details
- Verified Reactivity
- Human
- Formulation
- Particle: Aqueous solution containing 0.05% sodium azide and 0.3% BSA.
- Preparation
- Conjugated nanobeads: Protein-coated magnetic beads.
- Storage & Handling
- All components should be stored undiluted between 2°C and 8°C
- Application
-
Cell Separation (MojoSort™) - Quality tested
- Recommended Usage
-
20 µL of True-Stain Monocyte Blocker™ for 1 x 107 cells in 100 µL of buffer.
10 µL of Human CD56 Nanobeads for 1 x 107 cells in 100 µL of buffer - Application Notes
-
This kit is designed for the positive selection or depletion of human CD56+ cells from PBMCs.
Each lot has been individually optimized. Do not mix and match components from different lots or different kits.
Nanobead dilution to use in column: 5x
Antigen Details
- Gene ID
- NA
- UniProt
- View information about CD56 on UniProt.org
Related FAQs
- Is there a way to detach your magnetic particles from the cell surface?
-
MojoSort™ Nanobeads: These magnetic particles cannot be removed from cells. This includes standalone Nanobeads and MojoSort kits using Nanobeads. We have found that cells are functional without the need to detach the magnetic Nanobeads.
MojoSort Microbeads: These magnetic particles may be released following the detailed product-specific protocol.
- What is the size of your magnetic particles?
-
MojoSort Nanobeads: the average diameter is approximately 130 nm.
MojoSort Microbeads: the average diameter is approximately 1 μm.
- Are MojoSort™ Nanobeads compatible with other commercially available magnetic separation systems?
-
MojoSort™ magnetic particles can be used with other commercially available magnetic separators, both free standing magnets and column-based systems. Because MojoSort™ protocols are optimized for the MojoSort™ separator, the protocols may need to be adjusted for other systems. Please contact BioLegend Technical Service for more product-specific information and guidance. We do not recommend using MojoSort™ particles for BD’s IMag™ or Life Technologies’ DynaMag™.
- What antibodies are present in the depletion cocktails provided for isolation kits?
-
Please contact our technical service team for further assistance.
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