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CD4+ T cells were isolated from anti-coagulated human whole blood using the MojoSort™ Whole Blood Human CD4 T Cell Isolation Kit. After separation, the cells were stained with anti-human CD3 (clone UCHT1) PE, anti-human CD4 (clone RPA-T4) APC, and anti-human CD45 (clone HI30) FITC. Doublets, dead cells, and non-CD45 cells were excluded from analysis. Purity of the isolated CD4 T cells ranges between 87-98% due to donor variation.
| Cat # | Size | Price | Quantity Check Availability | Save | ||
|---|---|---|---|---|---|---|
| 480161 | 10 tests | 140€ | ||||
| 480162 | 100 tests | 416€ | ||||
Non-CD4 T cells are depleted by incubating the sample with a biotin antibody cocktail followed by incubation with magnetic Streptavidin Nanobeads. The magnetically labeled fraction is retained by the use of a magnetic separator. The untouched CD4 T cells are collected by decanting the liquid into a clean tube. These are the cells of interest; do not discard the liquid. Some of the downstream applications include functional assays, gene expression, phenotypic characterization, etc.
MojoSort™ reagents are also compatible with column-based cell separation systems available from other vendors. Optimized protocols for cell separation using columns from in-house testing are provided for each kit under the “Related Protocols” section, as well as representative data on the product webpage (where available). Data generated using column separators are indicated on the figure legend.
Due to the property of the beads, MojoSort™ reagents typically require dilution for optimal use on column separators. Where available, recommended dilution factors for each kit component based on in-house testing are provided under the “Application Notes” section of the webpage
Kit Contents
- Kit Contents
-
For Cat. No. 480161:
- 1 vial containing 200 µL of MojoSort Whole Blood Human CD4 T Cell Biotin-Antibody Cocktail
- 1 vial containing 200 µL of Streptavidin Nanobeads
For Cat. No. 480162:
- 2 vials containing 1 mL each of each of MojoSort Whole Blood Human CD4 T Cell Biotin-Antibody Cocktail
- 2 vials containing 1 mL each of Streptavidin Nanobeads
Product Details
- Verified Reactivity
- Human
- Formulation
-
Cocktail: Phosphate buffer solution containing 0.09% sodium azide, 0.5% BSA and stabilizer, pH 7.2.
Particle: Aqueous solution containing BSA and 0.05% sodium azide. - Preparation
-
The antibodies were purified by affinity chromatography, and conjugated with biotin under optimal conditions. The solution is free of unconjugated biotin.
Streptavidin Nanobeads: Streptavidin-coated magnetic beads. - Storage & Handling
- All components should be stored undiluted between 2°C and 8°C.
- Application
-
Cell Separation (MojoSort™) - Quality tested
- Recommended Usage
-
20 µL of Biotin-Antibody Cocktail for 1 x 107 cells in 100 µL of buffer.
20 µL of Streptavidin Nanobeads for 1 x 107 cells in 100 µL of buffer. - Application Notes
-
This kit is designed for the isolation of untouched CD4+ T cells from freshly drawn anticoagulated whole blood.
Each lot has been individually optimized. Do not mix and match components from different lots or different kits.
Antibody or cocktail dilution to use in column: 2X
Nanobead dilution to use in column: 1X
Antigen Details
- Gene ID
- NA
- UniProt
- View information about CD4 on UniProt.org
Related FAQs
- Is there a way to detach your magnetic particles from the cell surface?
-
MojoSort™ Nanobeads: These magnetic particles cannot be removed from cells. This includes standalone Nanobeads and MojoSort kits using Nanobeads. We have found that cells are functional without the need to detach the magnetic Nanobeads.
MojoSort Microbeads: These magnetic particles may be released following the detailed product-specific protocol.
- What is the size of your magnetic particles?
-
MojoSort Nanobeads: the average diameter is approximately 130 nm.
MojoSort Microbeads: the average diameter is approximately 1 μm.
- Are MojoSort™ Nanobeads compatible with other commercially available magnetic separation systems?
-
MojoSort™ magnetic particles can be used with other commercially available magnetic separators, both free standing magnets and column-based systems. Because MojoSort™ protocols are optimized for the MojoSort™ separator, the protocols may need to be adjusted for other systems. Please contact BioLegend Technical Service for more product-specific information and guidance. We do not recommend using MojoSort™ particles for BD’s IMag™ or Life Technologies’ DynaMag™.
- What antibodies are present in the depletion cocktails provided for isolation kits?
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Please contact our technical service team for further assistance.
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