Pacific Blue anti-human CD326 EpCAM Antibody anti-CD326

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Clone: 9C4 (See other available formats)
Regulatory Status: RUO
Other Names: Ep-CAM, tumor associated calcium signal transducer 1, epithelial cell surface antigen, epithelial glycoprotein 2, EGP2, adenocarcinoma associated antigen, TROP1
Isotype: Mouse IgG2b, κ
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Product Citations: publications

9C4_PB_090310 — Human colon carcinoma cell line (HT29) stained with 9C4 Pacific Blue™

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324217

25 tests

118€

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324218

100 tests

249€

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Description

CD326 is also known as Ep-CAM, tumor associated calcium signal transducer 1, epithelial cell surface antigen, epithelial glycoprotein 2, EGP2, adenocarcinoma associated antigen, and TROP1. CD326 is a type I transmembrane protein containing six disulfide bridges and one THYRO domain. This cell surface glycosylated 40 kD protein is highly expressed in bone marrow, colon, lung, and most normal epithelial cells and is expressed on carcinomas of gastrointestinal origin. Recently, it has been reported that CD326 expression occurs during the early steps of erythrogenesis. CD326 functions as a homotypic calcium-independent cell adhesion molecule and is believed to be involved in carcinogenesis by its ability to induce genes involved in cellular metabolism and proliferation. CD326 antigen is an immunotherapeutic target for the treatment of human carcinomas.

Product Details

Technical Data Sheet (pdf)

Product Details

Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: DU.4475 breast carcinoma
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography, and conjugated with Pacific Blue™ under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

* Pacific Blue™ has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue™ conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.

Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser: Violet Laser (405 nm)
Application Notes: Additional reported applications (for the revelant formats) include: immunofluorescence, immunohistochemistry³, and spatial biology (IBEX)^4,5.
RRID: AB_10642820 (BioLegend Cat. No. 324217)
AB_10642820 (BioLegend Cat. No. 324218)

Antigen Details

Structure: Type I transmembrane protein, contains six disulfide bridges, one THYRO domain, approximate molecular weight 40 kD.
Distribution: Highly expressed in bone marrow, colon, lung, and most normal epithelial cells. Also highly expressed on carcinomas of gastrointestinal origin. Expressed during early erythrogenesis.
Function: Homotypic calcium-independent cell adhesion. CD326 is believed to be involved in carcinogenesis by its ability to induce genes involved in cellular metabolism and proliferation.
Modification: Glycosylated.
Cell Type: Embryonic Stem Cells, Epithelial cells
Biology Area: Cell Biology, Immunology, Stem Cells
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. Strnad J, et al. 1989. Cancer Res. 49:314.
2. Munz M, et al. 2004. Oncogene 23:5748.
3. Rao CG, et al. 2005. Int. J. Oncol. 27:49.
Gene ID: 4072 View all products for this Gene ID
UniProt: View information about CD326 on UniProt.org

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Cell Surface Flow Cytometry Staining Protocol

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Other Formats

View All CD326 Reagents Request Custom Conjugation

Description	Clone	Applications
Purified anti-human CD326 (EpCAM)	9C4	FC,ICC,IHC
FITC anti-human CD326 (EpCAM)	9C4	FC
PE anti-human CD326 (EpCAM)	9C4	FC
APC anti-human CD326 (EpCAM)	9C4	FC
Alexa Fluor® 488 anti-human CD326 (EpCAM)	9C4	FC,SB
Alexa Fluor® 647 anti-human CD326 (EpCAM)	9C4	FC,ICC,SB
PerCP/Cyanine5.5 anti-human CD326 (EpCAM)	9C4	FC,SB
Biotin anti-human CD326 (EpCAM)	9C4	FC
Pacific Blue™ anti-human CD326 (EpCAM)	9C4	FC
Brilliant Violet 421™ anti-human CD326 (EpCAM)	9C4	FC
PE/Cyanine7 anti-human CD326 (EpCAM)	9C4	FC
Brilliant Violet 605™ anti-human CD326 (EpCAM)	9C4	FC
Brilliant Violet 650™ anti-human CD326 (EpCAM)	9C4	FC
Alexa Fluor® 594 anti-human CD326 (EpCAM)	9C4	IHC-P,ICC,SB
Purified anti-human CD326 (EpCAM) (Maxpar® Ready)	9C4	FC,CyTOF®
PE/Dazzle™ 594 anti-human CD326 (EpCAM)	9C4	FC
APC/Fire™ 750 anti-human CD326 (EpCAM)	9C4	FC
Brilliant Violet 510™ anti-human CD326 (EpCAM)	9C4	FC
Brilliant Violet 785™ anti-human CD326 (EpCAM)	9C4	FC
Brilliant Violet 711™ anti-human CD326 (Ep-CAM)	9C4	FC
TotalSeq™-A0123 anti-human CD326 (Ep-CAM)	9C4	PG
APC/Cyanine7 anti-human CD326 (Ep-CAM)	9C4	FC
Alexa Fluor® 700 anti-human CD326 (EpCAM)	9C4	FC
TotalSeq™-C0123 anti-human CD326 (Ep-CAM)	9C4	PG
TotalSeq™-B0123 anti-human CD326 (Ep-CAM)	9C4	PG
PE/Cyanine5 anti-human CD326 (Ep-CAM)	9C4	FC
TotalSeq™-D0123 anti-human CD326 (Ep-CAM)	9C4	PG
Spark UV™ 387 anti-human CD326 (Ep-CAM)	9C4	FC
Spark Red™ 718 anti-human CD326 (Ep-CAM) (Flexi-Fluor™)	9C4	FC
Spark Blue™ 574 anti-human CD326 (Ep-CAM) (Flexi-Fluor™)	9C4	FC
Spark Blue™ 550 anti-human CD326 (Ep-CAM) (Flexi-Fluor™)	9C4	FC

Certificate of Analysis

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Go To Top Version: 1 Revision Date: 11.30.2012

For Research Use Only. Not for diagnostic or therapeutic use.

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