- Clone
- 16B10B53 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- BTB and CNC homolog 2, Transcription regulator protein BACH2,
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
695603 | 25 tests | 172€ | ||||
695604 | 100 tests | 336€ |
BTB and CNC Homolog 2 (BACH2) is a transcription factor of the basic leucine zipper family. BACH2 participates in the regulation of both innate and adaptive immune responses. In B cells, BACH2 plays critical roles in somatic hypermutation, class-switch recombination, memory B cell formation, and repression of myeloid genes. In T cells, BACH2 promotes the development of Foxp3+ regulatory T cells and memory T cells. Bach2 is required for the maintenance of naive CD4 T cells by suppressing effector T cell differentiation. BACH2 is also required for the maturation and function of alveolar macrophages. Defects in BACH2 gene are associated with autoimmune and allergic diseases, such as asthma, type 1 diabetes, celiac disease, rheumatoid arthritis, and generalized vitiligo.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Human BACH2 recombinant protein (513-841 a.a.) expressed in E. coli.
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
ICFC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
- Excitation Laser
-
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- RRID
-
AB_2922645 (BioLegend Cat. No. 695603)
AB_2922645 (BioLegend Cat. No. 695604)
Antigen Details
- Structure
- 841 amino acids with a predicted molecular weight of 92.5 kD. Contains a N-terminal BTB/POZ domain for protein-protein interaction and a C-terminal bZIP domain for DNA binding.
- Distribution
-
Nucleus and cytoplasm.
- Function
- BACH2 is a transcription factor involved in the development and differentiation of both B and T cells.
- Interaction
- Forms homodimers or heterodimers with Maf-related transcription factors.
- Cell Type
- B cells, Tregs
- Biology Area
- Cell Biology, Immunology, Transcription Factors
- Molecular Family
- Nuclear Markers
- Antigen References
-
1. Kuwahara M, et al. 2016. Nat. Commun. 7:12596.
2. Sidwell T, et al. 2016. Nat. Immunol. 17:744.
3. Roychoudhuri R, et al. 2016. Nat. Immunol. 17:851.
4. Itoh-Nakadai A, et al. 2014. Nat. Immunol. 15:1171.
5. Kuwahara M, et al. 2014. Nat. Commun. 5:3555.
6. Kim EH, et al. 2014. J. Immunol. 192:985. - Gene ID
- 60468 View all products for this Gene ID
- UniProt
- View information about BACH2 on UniProt.org
Related Pages & Pathways
Pages
Related FAQs
- What type of PE do you use in your conjugates?
- We use R-PE in our conjugates.
Other Formats
View All BACH2 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-BACH2 | 16B10B53 | WB |
PE anti-BACH2 | 16B10B53 | ICFC |
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Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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-
Purified anti-BACH2
Total cell lysates (15µg protein) from THP1 and Raji (negati... -
PE anti-BACH2
Ramos cells were fixed and permeabilized with Cyto-Fast™ Fix...
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