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PE anti-human CD156c (ADAM10) Antibody

Pricing & Availability
Clone
SHM14 (See other available formats)
Regulatory Status
RUO
Other Names
MADM, KUZ, alpha-secretase
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
SHM14_PE_090111
Human peripheral blood lymphocytes were stained with ADAM10 (SHM14) PE (filled histogram) or mouse IgG1, κ PE isotype control (open histogram).
  • SHM14_PE_090111
    Human peripheral blood lymphocytes were stained with ADAM10 (SHM14) PE (filled histogram) or mouse IgG1, κ PE isotype control (open histogram).
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352703 25 tests 123€
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352704 100 tests 288€
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Description

CD156c, also known as a disintegrin and metalloproteinase domain-containing protein 10 (ADAM10), is a 748 amino acid type I membrane glycoprotein ubiquitously expressed on most cell types.  It consists of multiple functional domains, including a N-terminal prodomain, catalytic domain, cysteine-rich domain, transmembranous domain, and cytoplasmic domain. It is secreted as a precursor protein and becomes as the activate/mature form through removing the ADAM10 prodomain by proprotein convertase 7 and furin. ADAM10 functions as metalloproteinase to cleave several molecules including Notch, pro-TNF-α, amyloid precursor protein, myelin basic protein, and type IV collagen. It mediates the release of several cell adhesion molecules such as vascular endothelial cadherin or L-selectin to regulate endothelial permeability and leukocyte transmigration. Dysregulation of ADAM activity may contribute to the pathogenesis of vascular diseases.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Reported Reactivity
African Green, Baboon, Cynomolgus, Rhesus
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human A375 cell lines
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Product Citations
  1. Lee JH, et al. 2022. EBioMedicine. 77:103903. PubMed
  2. DeBell KE, et al. 2012. BMC Immunol. 23:13. PubMed
  3. Finkin S, et al. 2015. Nat Immunol. 1235:16. PubMed
  4. Shambat S, et al. 2015. Dis Model Mech. 8: 1413 - 1425. PubMed
  5. Popov L, et al. 2015. Proc Natl Acad Sci U S A. 10.1073/pnas.1510265112. PubMed
  6. Franke M, et al. 2016. J Biol Chem. 291: 10551 - 10561. PubMed
  7. Riethmueller S, et al. 2016. Sci Rep. 6: 25550. PubMed
  8. Popov L, et al. 2015. Proc Natl Acad Sci U S A. 112: 14337 - 14342. PubMed
  9. Triplett KD, et al. 2019. Sci Rep. 9:1343. PubMed
  10. Ostalecki C, et al. 2016. EBioMedicine. 13:294-304. PubMed
RRID
AB_10896790 (BioLegend Cat. No. 352703)
AB_10896790 (BioLegend Cat. No. 352704)

Antigen Details

Structure
Consist of signal sequence, a N-terminal prodomain followed by a catalytic proteinase domain containing a zinc-binding motif, a disintegrin, cysteine-rich transmembrane, and a cytoplasmatic domain
Distribution

Ubiquitously expressed on most of the cell types

Function
Acts as an efficient α-secretase
Interaction
Proteolysis the amyloid precursor protein (APP)
Cell Type
B cells, Dendritic cells, Tregs
Biology Area
Cell Adhesion, Cell Biology, Immunology, Innate Immunity, Neurodegeneration, Neuroscience, Protein Misfolding and Aggregation, Signal Transduction
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Edwards DR, et al. 2008. Mol. Aspects Med. 29:258.
2. Anders A, et al. 2001. FASEB J. 15:1837.
3. Endres K, et al. 2010. FEBS J. 277:1585.
4. Mochizuki S, et al. 2007. Cancer Sci. 98:621.

Gene ID
4684 View all products for this Gene ID
UniProt
View information about CD156c on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 11.30.2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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