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PE anti-human CD304 (Neuropilin-1) Antibody

Pricing & Availability
Clone
12C2 (See other available formats)
Regulatory Status
RUO
Other Names
BDCA-4
Isotype
Mouse IgG2a, κ
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Product Citations
publications
12C2_PE_1_060512
Human peripheral blood mononuclear cells were stained with CD303 APC and CD304 (clone 12C2) PE (top) or mouse IgG2a, κ PE isotype control (bottom). Data shown was gated on the lymphocyte and monocyte populations.
  • 12C2_PE_1_060512
    Human peripheral blood mononuclear cells were stained with CD303 APC and CD304 (clone 12C2) PE (top) or mouse IgG2a, κ PE isotype control (bottom). Data shown was gated on the lymphocyte and monocyte populations.
  • 12C2_PE_2_060512
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354503 25 tests 123€
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354504 100 tests 288€
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Description

CD304, also known as neuropilin-1, BDCA-4 and VEGF165R, is a 140 kD type I transmembrane protein. Its extracellular region contains 2 CUB, 2 FV/FVIII, and one MAM domain; a soluble isoform is produced by alternative mRNA splicing. CD304 is involved in angiogenesis, neural development, and tumor metastasis. It's expressed by plasmacytoid dendritic cells, thymocytes, neurons, endothelium, and a subset of TFH cells. CD304 is also expressed in several carcinomas, and a high expression of this molecule in prostate cancer correlates with a poor prognosis.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
CD304-Fc Fusion protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Product Citations
  1. Soday L, et al. 2021. Frontiers in Immunology. 12:600056. PubMed
  2. Cignarella F et al. 2018. Cell metabolism. 27(6):1222-1235 . PubMed
  3. Argüello RJ, et al. 2020. Cell Metab. 32:1063. PubMed
  4. Tandon S, et al. 2022. Br J Haematol. 196:e34. PubMed
  5. Combes AJ, et al. 2022. Cell. 185:184. PubMed
  6. Kitai H, et al. 2022. BMC Biol. 20:248. PubMed
  7. Leclerc M, et al. 2019. Nat Commun. 10:3345. PubMed
  8. Cillo AR, et al. 2021. Cell Rep Med. 2:100476. PubMed
  9. Mujal AM, et al. 2022. Cancer Immunol Res. 10:403. PubMed
  10. Congy-Jolivet N, et al. 2022. EBioMedicine. 80:104047. PubMed
  11. Leylek R, et al. 2020. Cell Rep. 32:108180. PubMed
RRID
AB_11219200 (BioLegend Cat. No. 354503)
AB_11219200 (BioLegend Cat. No. 354504)

Antigen Details

Structure
Type I transmembrane protein, 140 kD, 2 complement binding domains (CUB), 2 coagulation factor V/VIII homology domains (FV/FVIII), one meprin, A5, receptor tyrosine phosphatase domain (MAM)
Distribution

Plasmacytoid dendritic cells, thymocytes, subset of follicular helper T cells (TFH), endothelial cells, neurons, some carcinomas

Function
Angiogenesis, neuronal development, tumor metastasis
Ligand/Receptor
VEGF165, semaphorin-3A
Cell Type
Dendritic cells, Endothelial cells, Neurons, Tfh, Thymocytes
Biology Area
Angiogenesis, Cell Adhesion, Cell Biology, Immunology, Innate Immunity, Neuroscience, Synaptic Biology
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Mizui M and Kikutani H. 2008. Immunity 28:302.
2. Hamerlik P, et al. 2012. J. Exp. Med. 209:507.
3. Karjalainen K, et al. 2011. Blood 117:920.
4. Lepelletier Y, et al. 2007. P. Natl. Acad. Sci. USA 104:5545.

Gene ID
8829 View all products for this Gene ID
UniProt
View information about CD304 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 11.30.2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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