- Clone
- MEM-55 (See other available formats)
- Regulatory Status
- RUO
- Workshop
- V CD45.08
- Other Names
- CD45RB
- Isotype
- Mouse IgG2b, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
Cat # | Size | Price | Quantity Check Availability | Save | ||
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310204 | 100 tests | 215€ |
CD45RB is a 180-240 kD single chain type I membrane glycoprotein. It is an exon 5 splice variant of the tyrosine phosphatase CD45. The CD45RB isoform is expressed on thymocytes, T cell subsets, B cells, monocytes/macrophages, granulocytes, and dendritic cells. CD45RB enhances both T cell receptor and B cell receptor signaling mediated activation. CD45 and its isoforms non-covalently associate with lymphocyte phosphatase-associated phosphoprotein (LPAP) on T and B lymphocytes. CD45 has been reported to be associated with several other cell surface antigens including CD1, CD2, CD3, and CD4. CD45 has also been reported to bind galectin-1. CD45 isoform expression can change in response to cytokines. The MEM-55 antibody recognizes a silaidase-sensitive epitope of CD45.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Reported Reactivity
- African Green, Baboon, Cynomolgus, Rhesus
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Human thymocytes and T lymphocytes
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
- Excitation Laser
-
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- Application Notes
-
The MEM-55 antibody is specific for a glycosylated-CD45RB epitope that is highly expressed on memory B cells and plasmablasts but not on naïve B cells. Additional reported applications (for the relevant formats) include: immunoprecipitation, Western blotting, and immunohistochemical staining of formalin-fixed paraffin-embedded tissue sections.
- Application References
-
- Horejsi V, et al. 1988. Folia Biol. 34:23.
- Bazil V, et al. 1989. Immunogenetics 29:202.
- Knapp W, et al. 1989. Leucocyte Typing IV. Oxford University Press. New York.
- Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
- Koethe S, et al. 2011. J. Leukoc. Biol. 90:5.
- Product Citations
-
- RRID
-
AB_314807 (BioLegend Cat. No. 310204)
Antigen Details
- Structure
- Tyrosine phosphatases, type I transmembrane, 180-240 kD (exon 5 splicing of CD45 gene)
- Distribution
-
Thymocytes, T cell subset, B cells, monocytes/macrophages, granulocytes, dendritic cells
- Function
- Enhances TCR and BCR signaling
- Ligand/Receptor
- Galectin-1, CD2, CD3, CD4
- Cell Type
- B cells, Dendritic cells, Granulocytes, Macrophages, Monocytes, T cells, Thymocytes, Tregs
- Biology Area
- Cell Biology, Immunology, Inhibitory Molecules, Neuroscience, Neuroscience Cell Markers
- Molecular Family
- CD Molecules
- Antigen References
-
1. Thomas M. 1989. Annu. Rev. Immunol. 7:339.
2. Trowbridge I, et al. 1994. Annu. Rev. Immunol.12:85. - Gene ID
- 5788 View all products for this Gene ID
- UniProt
- View information about CD45RB on UniProt.org
Related Pages & Pathways
Pages
Related FAQs
- What type of PE do you use in your conjugates?
- We use R-PE in our conjugates.
Other Formats
View All CD45RB Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
PE anti-human CD45RB | MEM-55 | FC |
Purified anti-human CD45RB | MEM-55 | FC,IHC-P,IP,WB |
FITC anti-human CD45RB | MEM-55 | FC |
Alexa Fluor® 594 anti-human CD45RB | MEM-55 | IHC-P |
TotalSeq™-A0844 anti-human CD45RB | MEM-55 | PG |
TotalSeq™-C0844 anti-human CD45RB Antibody | MEM-55 | PG |
TotalSeq™-B0844 anti-human CD45RB | MEM-55 | PG |
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Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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PE anti-human CD45RB
Human peripheral blood lymphocytes stained with MEM-55 PE -
Purified anti-human CD45RB
Human peripheral blood lymphocytes stained with purified MEM... Human paraffin-embedded tonsil tissue slices were prepared w... -
FITC anti-human CD45RB
Human peripheral blood lymphocytes stained with FITC anti-hu... -
Alexa Fluor® 594 anti-human CD45RB
Human paraffin-embedded tonsil tissue slices were prepared w... -
TotalSeq™-A0844 anti-human CD45RB
-
TotalSeq™-C0844 anti-human CD45RB Antibody
-
TotalSeq™-B0844 anti-human CD45RB
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