- Clone
- S20004A (See other available formats)
- Regulatory Status
- RUO
- Other Names
- FcγRIIa
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
Cat # | Size | Price | Quantity Check Availability | Save | ||
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365503 | 25 tests | 94€ | ||||
365504 | 100 tests | 198€ |
CD32A (FCGR2A) is a 40 kD, type I protein with 2 C2-set domains the extracellular region and 1 ITAM in the intracellular region. CD32A binds monomeric IgG with low affinity but is very efficient binding immune complexes. FCGR2A is expressed by myeloid cells such as macrophages, monocytes, neutrophils, eosinophils and dendritic cells; CD32a is also expressed by epithelial cells, platelets and activated CD4+ T cells. CD32a is involved in phagocytosis and clearing of immune complexes.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- FCGR2A-transfected cells
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
- Excitation Laser
-
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- Application Notes
-
This clone does not recognizes CD32B-transfected cell
- RRID
-
AB_2904394 (BioLegend Cat. No. 365503)
AB_2904394 (BioLegend Cat. No. 365504)
Antigen Details
- Structure
- Member of the Ig superfamily, 40 kD, type I protein, 2 C2-set domains, 1 ITAM
- Distribution
-
Macrophages, monocytes, neutrophils, eosinophil, platelets, and CD4+ T Cells
- Function
- Phagocytosis, cytotoxicity, platelet aggregation
- Ligand/Receptor
- Aggregated IgG, immune complexes
- Cell Type
- Eosinophils, Granulocytes, Macrophages, Monocytes, Neutrophils, Platelets
- Biology Area
- Adaptive Immunity, Immunology
- Molecular Family
- CD Molecules, Fc Receptors
- Antigen References
-
- Hubbard J. et al. 2020. J Exp Med. 217:e20200359
- Newling M, et al. 2020. Clin Exp Immunol. 199:39-49
- Maria H, et al. 2018. Front Immunol. 9:2814
- Descours B, et al. 2017. Nature. 543:564-567
- Gene ID
- NA
- UniProt
- View information about FCGR2A/CD32A on UniProt.org
Related Pages & Pathways
Pages
Related FAQs
- What type of PE do you use in your conjugates?
- We use R-PE in our conjugates.
Other Formats
View All FCGR2A/CD32A Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
PE anti-human FCGR2A/CD32A | S20004A | FC |
Purified anti-human FCGR2A/CD32A | S20004A | FC |
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Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
-
PE anti-human FCGR2A/CD32A
Human peripheral blood monocytes stained with anti-human FCG... -
Purified anti-human FCGR2A/CD32A
Human peripheral blood monocytes stained with purified anti-...
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