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PE anti-mouse EOMES Antibody

Pricing & Availability
Clone
W17001A (See other available formats)
Regulatory Status
RUO
Other Names
Eomesodermin, T-box brain protein 2 (TBR-2)
Isotype
Rat IgG2b, λ
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Product Citations
publications
W17001A_PE_EOMES_Antibody_120220
C57BL/6 mouse splenocytes were stained with anti-mouse CD8a FITC then treated with True-Nuclear Transcription Factor Buffer Set. Cells were then stained with anti-mouse EOMES (clone W17001A) PE (left) or rat IgG2b, κ PE isotype control (right).
  • W17001A_PE_EOMES_Antibody_120220
    C57BL/6 mouse splenocytes were stained with anti-mouse CD8a FITC then treated with True-Nuclear Transcription Factor Buffer Set. Cells were then stained with anti-mouse EOMES (clone W17001A) PE (left) or rat IgG2b, κ PE isotype control (right).
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157705 25 µg 113€
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157706 100 µg 235€
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Description

Eomesodermin, or EOMES, is a transcription factor in the T-box family. During embryonic development, EOMES is crucial in regulating trophoblast differentiation, gastrulation, mesoderm delamination, and development of the cerebral cortex. In the immune system, EOMES is involved in the activation, migration, and differentiation of CD8+ T cells. In cooperation with another T-box transcription factor, T-bet, EOMES induces production of IFN-γ and enhances cytotoxic activities of effector CD8+ T cells. EOMES has been shown to be required to maintain long-term memory of CD8+ T cells and is important for homeostasis of memory and effector T cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse EOMES recombinant protein (463-707 a.a.) expressed in E. coli.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
0.2 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.06 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

W17001A was tested for ICC using 4% PFA-fixed HeLa (negative control) and NK-92 (positive control cells) permeabilized with either Triton X-100 or methanol. Both methods resulted in high non-specific staining in HeLa cells. We therefore don’t recommend W17001A for this application.

Product Citations
  1. Cheng C, et al. 2022. Cell Mol Gastroenterol Hepatol. 15:261. PubMed
RRID
AB_2888891 (BioLegend Cat. No. 157705)
AB_2888891 (BioLegend Cat. No. 157706)

Antigen Details

Distribution

Nucleus

Antigen References
  1. Cooper L, et al. 2018. Plos One. 13(12).
  2. Pikovskaya O, et al. 2016. J.Immunol. 196(4):1449-54.
  3. Lupar E, et al. 2015. J.Immunol. 195(10):4742-52.
Gene ID
13813 View all products for this Gene ID
UniProt
View information about EOMES on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 12.02.2020

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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