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PE anti-STAT5 Phospho (Tyr694) Antibody

Pricing & Availability
Clone
A17016B.Rec (See other available formats)
Regulatory Status
RUO
Other Names
Signal transducer and activator of transcription 5A
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
A17016BRec_PE_STAT5-Phospho_Antibody_031720.png
Human peripheral blood mononuclear cells were treated with (left), or without (right) recombinant human IL-2 for 15 minutes, fixed with Fixation Buffer (Cat. No. 420801), permeabilized with True-Phos™ Perm Buffer (Cat. No. 425401) then stained with CD20 APC and anti-STAT5 Phospho (Tyr694) (Clone A17016B.Rec) PE. Data was gated on the lymphocyte population.
  • A17016BRec_PE_STAT5-Phospho_Antibody_031720.png
    Human peripheral blood mononuclear cells were treated with (left), or without (right) recombinant human IL-2 for 15 minutes, fixed with Fixation Buffer (Cat. No. 420801), permeabilized with True-Phos™ Perm Buffer (Cat. No. 425401) then stained with CD20 APC and anti-STAT5 Phospho (Tyr694) (Clone A17016B.Rec) PE. Data was gated on the lymphocyte population.
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936903 25 tests 123€
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936904 100 tests 288€
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Description

STAT5 is a member of the signal transducer and activator of transcription factors (STAT) family, mediating growth and cytokine signaling. STAT5 consists of two closely related family members, STAT5A and STAT5B, which exhibit 96% sequence homology and are functionally redundant. Upon activation, STAT5 is phosphorylated by receptor tyrosine kinases and, in turn, forms homodimers or hererodimers with other family members through its SH2 domains. The dimerized STAT5 translocates to the nucleus and binds to the STAT5 response element (TTCXXXGAA). STAT5 plays crucial regulatory roles in cell proliferation, anti-apoptosis, early B cell differentiation, and T cell development.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Recombinant
Host Species
Mouse
Immunogen
Peptide from Human Stat5A phosphorylated at Tyr694
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

This recombinant antibody clone is derived from clone A17016B. No changes or modifications were done to the antibody sequence or scaffold.

Product Citations
  1. Hossain MA, et al. 2022. Cell Rep. 39:111021. PubMed
  2. Ringelstein-Harlev S, et al. 2023. Eur J Haematol. 110:371. PubMed
RRID
AB_2832912 (BioLegend Cat. No. 936903)
AB_2832912 (BioLegend Cat. No. 936904)

Antigen Details

Structure
794 amino acids, predicted molecular weight of 91 kD; contains a SH2 domains responsible for homodimerization or heterodimerization.
Distribution

Cytoplasm; phosphorylated form translocates to the nucleus.

Function
STAT5 is a transcription regulator and is involved in cell differentiation, proliferation, and survival, in response to several cytokines, such as interleukins, erythropoietin, growth hormone, thrombopoietin, and prolactin.
Antigen References
  1. Bell BD, et al. 2013. Nat. Immunol. 14:364.
  2. Cholez E, et al. 2012. Leukemia. 26:2390.
  3. Heltemes-Harris LM, et al. 2012. Curr. Opin. Immunol. 24:146.
  4. Heltemes-Harris LM, et al. 2011. Ann. N Y Acad. Sci. 1217:18.
  5. Malin S, et al. 2010. Curr. Opin. Immunol. 22:168.
  6. Xu J, et al. 2009. J. Neurosci. 29:2022.
Gene ID
6776 View all products for this Gene ID
UniProt
View information about STAT5 Phospho Tyr694 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 03.17.2020

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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