PE/Cyanine7 anti-human CD243 MDR-1 Antibody anti-CD243

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Clone: UIC2 (See other available formats)
Regulatory Status: RUO
Other Names: Multidrug resistance protein 1, ATP-binding cassette sub-family B member 1, P-glycoprotein 1
Isotype: Mouse IgG2a, κ
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Product Citations: publications

Human neuroblastoma cell line, SK-N-FI, was stained with human CD243 (clone UIC2) PE/Cyanine7 (filled histogram) or mouse IgG2a, κ PE/Cyanine7 isotype control (open histogram).

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348610

100 tests

278€

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Description

CD243 (MDR-1) belongs to the ATP binding cassette (ABC) transporter family. With an approximate molecular mass of 170 kD, it consists of two homologous halves. Each half contains two hydrophobic transmembrane domains (TMDs) and two hydrophilic nucleotide binding domains (NBDs). The TMDs span the membrane six times, forming a chamber with a 12 transmembrane α-helix structure. NBDs drive the transport process through ATP coupling and hydrolysis and are located at the cytoplasmic face of the membrane. CD243 transports various molecules across cellular membranes and is involved in multidrug resistance. MDR-1 is expressed on hematopoietic stem cells, T cells, B cells, and NK cells as well as on many multidrug resistant neoplastic cells. CD243 interacts with Caveolin, RING finger protein 1B, AAP1, p53, Orphan nuclear receptor PX, and cytochrome P450.

Product Details

Technical Data Sheet (pdf)

Product Details

Verified Reactivity: Human
Reported Reactivity: African Green, Baboon
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: NIH 3T3 cells transfected with human MDR-1 cDNA
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with PE/Cyanine7 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: Additional reported applications (for the relevant formats) include: blocking the efflux of fluorescent dyes^1,2, immunoprecipitation¹, and immunohistochemical staining of tissue sections of squamous cell carcinoma³.
RRID: AB_2562764 (BioLegend Cat. No. 348610)

Antigen Details

Structure: Member of ABC transporters family, MW ~170 kD. Consists of two homologous halves, each comprising two hydrophobic transmembrane domains (TMDs) and two hydrophilic nucleotide binding domains (NBDs).
Distribution: Hematopoietic stem cells, T and B cells, natural killer (NK) cells, and many multidrug resistant neoplastic cells.
Function: Transport various molecules across cellular membranes. Involved in multidrug resistance.
Cell Type: B cells, Hematopoietic stem and progenitors, NK cells, T cells
Biology Area: Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Immunology
Molecular Family: CD Molecules
Antigen References: 1. Dulucq S, et al. 2008. Blood 112:2024.
2. Gottesman MM, et al. 2009. Nat. Biotechnol. 27:546.
3. Andreadis C, et al. 2007. Blood 109:3409.
Gene ID: 5243 View all products for this Gene ID
UniProt: View information about CD243 on UniProt.org

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Other Formats

View All CD243 Reagents Request Custom Conjugation

Description	Clone	Applications
PE/Cyanine7 anti-human CD243 (MDR-1)	UIC2	FC
Purified anti-human CD243 (MDR-1)	UIC2	FC,IHC-P,IP
PE anti-human CD243 (MDR-1)	UIC2	FC
APC anti-human CD243 (MDR-1)	UIC2	FC
PerCP/Cyanine5.5 anti-human CD243 (MDR-1)	UIC2	FC
Alexa Fluor® 647 anti-human CD243 (MDR-1)	UIC2	FC,IHC-P
Alexa Fluor® 594 anti-human CD243 (MDR-1)	UIC2	IHC-P
Brilliant Violet 421™ anti-human CD243 (MDR-1)	UIC2	FC

Certificate of Analysis

Go To Top Version: 1 Revision Date: 11.19.2013

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

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