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PE/Cyanine7 anti-mouse CD154 Antibody

Pricing & Availability
Clone
MR1 (See other available formats)
Regulatory Status
RUO
Other Names
CD40 ligand (CD40L), gp39, T-BAM, TRAP, Ly-62, TNFSF5
Isotype
Armenian Hamster IgG
Ave. Rating
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Product Citations
publications
MR1_PECy7_CD154_Antibody_FC_042414.jpg
Enriched C57BL/6 mouse splenic T cells were stimulated with PMA+ionomycin for 6 hours and then stained with CD154 (clone MR1) PE/Cyanine7 (filled histogram) or Armenian hamster IgG PE/Cyanine7 (open histogram).
  • MR1_PECy7_CD154_Antibody_FC_042414.jpg
    Enriched C57BL/6 mouse splenic T cells were stimulated with PMA+ionomycin for 6 hours and then stained with CD154 (clone MR1) PE/Cyanine7 (filled histogram) or Armenian hamster IgG PE/Cyanine7 (open histogram).
Compare all formats See PE/Cyanine7 spectral data
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106511 25 µg 100€
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106512 100 µg 231€
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Description

CD154 is a 39 kD TNF superfamily member also known as CD40 ligand, gp39, T-BAM, TRAP, and Ly-62. CD154 is an accessory molecule expressed predominantly on activated CD4+ lymphocytes that bind CD40. CD154 plays an important role in T-B cell costimulation. The MR1 antibody has been reported to inhibit the activation of T and B lymphocytes in vitro and antigen-specific lymphocyte responses in vivo.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Armenian Hamster
Immunogen
Activated mouse Th1 clone D1.6
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with PE/Cyanine7 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining1,2 of acetone-fixed frozen sections, and in vitro and in vivo blocking of ligand binding3-5. For most successful immunofluorescent staining results, it may be important to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate (Cat. No. 106506) or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated antibody (Cat. No. 106504) or biotinylated anti-Armenian hamster IgG (Cat. No. 405501) second step, followed by SAv-PE (Cat. No. 405204). The Ultra-LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 106515-106520).

Application References
  1. Lettesjö H, et al. 2000. J. Immunol. 165:4095. (IHC)
  2. Dunn RJ, et al. 1997. J. Histochem. Cytochem. 45:129. (IHC)
  3. Noelle RJ, et al. 1992. P. Natl. Acad. Sci. USA 89:6550. (Block)
  4. Roy M, et al. 1995. Eur. J. Immunol. 25:596. (Block)
  5. Foy TM, et al. 1994. J. Exp. Med. 180:157. (Block)
  6. Lawson BR, et al. 2007. J. Immunol. 178:5366.
Product Citations
  1. Kuczynski EA, et al. 2022. EMBO Mol Med. 14:e15816. PubMed
  2. Weng S, et al. 2022. Front Immunol. 13:1025931. PubMed
  3. Wang R, et al. 2023. NPJ Vaccines. 8:6. PubMed
  4. Pankhurst TE, et al. 2023. Cell Rep. 42:112310. PubMed
  5. Nording H, et al. 2021. Nat Commun. 12:3352. PubMed
  6. Birkner K, et al. 2019. Front Neurol. 0.559027778. PubMed
RRID
AB_2563493 (BioLegend Cat. No. 106511)
AB_2563493 (BioLegend Cat. No. 106512)

Antigen Details

Structure
TNF superfamily, 39 kD
Distribution

Activated CD4+ T cells

Function
T-B cell costimulation
Ligand/Receptor
CD40
Cell Type
T cells, Tregs
Biology Area
Costimulatory Molecules, Immunology
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Noelle RJ, et al. 1992. P. Natl. Acad. Sci. USA 89:6550.
3. Bancherou J, et al. 1994. Annu. Rev. Immunol. 12:881.
4. Clark EA, et al. 1996. P. Natl. Acad. Sci. USA 83:4494.

Gene ID
21947 View all products for this Gene ID
UniProt
View information about CD154 on UniProt.org
Go To Top Version: 1    Revision Date: 04.29.2014

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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