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PE/Cyanine7 anti-mouse CD69 Antibody

Pricing & Availability
Clone
H1.2F3 (See other available formats)
Regulatory Status
RUO
Other Names
Very Early Activation Antigen (VEA), AIM, EA1, MLR3, gp34/28
Isotype
Armenian Hamster IgG
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Product Citations
publications
H1dot2F3_PECy7_091907
Con A-stimulated Balb/c mouse splenocytes (24 hours) stained with H1.2F3 PE/Cyanine7
  • H1dot2F3_PECy7_091907
    Con A-stimulated Balb/c mouse splenocytes (24 hours) stained with H1.2F3 PE/Cyanine7
Compare all formats See PE/Cyanine7 spectral data
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104511 25 µg 62€
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104512 100 µg 152€
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Description

CD69 is a 60 kD type II membrane protein composed of a 27/33 kD disulfide-linked homodimer, also known as Very Early Activation Antigen (VEA), AIM, EA1, MLR3, and gp34/28. It is expressed on a subset of thymocytes and platelets. CD69 is rapidly induced on activated T and B cells, neutrophils, and NK cells. It is a C-type lectin, closely related to the NKR-P1 and Ly-49 NK cell activation molecules. CD69 is involved in the early events of cell activation and thymocyte positive selection.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Armenian Hamster
Immunogen
Mouse dendritic epidermal T cell line Y245
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE/Cyanine7 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

The H1.2F3 antibody has been reported to augment T cell activation. Additional reported applications (for the relevant formats) include: in vitro T cell and NK cell activation1-3, immunohistochemistry4,5, and immunoprecipitation1.

This antibody has been characterized in the literature as containing a lambda (?) light chain.

Application References
  1. Yokoyama WM, et al. 1988. J. Immunol. 141:369. (IP)
  2. Sobel ES, et al. 1993. J. Immunol. 150:673.
  3. Karlhofer FM, et al. 1991. J. Immunol. 146:3662.
  4. Zhou X, et al. 2005. J. Biol. Chem. 280:31240. (IHC)
  5. Podd BS, et al. 2006. J. Immunol. 176:6532. (IHC)
  6. Lawson BR, et al. 2007. J. Immunol. 178:5366.
  7. Lee JW, et al. 2006. Nature Immunol. 8:181.
  8. Epardaud M, et al. 2008. Cancer Res. 15:2972. PubMed
  9. Jordan JM, et al. 2008. 76:3717. PubMed
  10. Kenna TJ, et al. 2008. Blood 111:2091. PubMed
  11. Ishikawa C, et al. 2013. Biochim Biophys Acta. 167:99. PubMed
Product Citations
  1. Oliver AJ, et al. 2020. Oncoimmunology. 9:1802979. PubMed
  2. Behr FM, et al. 2021. Eur J Immunol. 51:151. PubMed
  3. Guttman O, et al. 2022. J Cell Biol. 221: . PubMed
  4. Grégoire C, et al. 2022. Immunity. 55:1216. PubMed
  5. Que W, et al. 2022. Sci Adv. 8:eabo4413. PubMed
  6. Zhu J, et al. 2022. Nat Commun. 13:7466. PubMed
  7. du Halgouet A, et al. 2023. Immunity. 56:78. PubMed
  8. Gonçalves R, et al. 2023. iScience. 26:105972. PubMed
  9. Wang J, et al. 2023. Inflamm Regen. 43:12. PubMed
  10. Fiege JK, et al. 2021. Cell Host Microbe. 29:1815. PubMed
  11. Hirai T, et al. 2020. Immunity. 54(1):84-98.e5. PubMed
  12. Saha D et al. 2017. Cancer cell. 32(2):253-267 . PubMed
  13. Konjar S, et al. 2022. Proc Natl Acad Sci U S A. 119:e2202144119. PubMed
  14. Wilfahrt D, et al. 2021. Elife. 10:. PubMed
  15. Israelow B, et al. 2020. bioRxiv. . PubMed
  16. Marco Barros R, et al. 2016. Cell. 167: 203-218. PubMed
  17. Redmann V, et al. 2016. Sci Rep. 6:23326. PubMed
  18. Muris A, et al. 2012. J Immunol Methods. 381:59. PubMed
  19. Bally AP, et al. 2017. J Immunol. 198:205. PubMed
  20. Wang L, et al. 2019. Cell Rep. 29:1848. PubMed
  21. Muthuswamy R, et al. 2021. J Immunother Cancer. 9:. PubMed
  22. Cannons JL, et al. 2021. Cell Rep. 37:109804. PubMed
  23. Manivasagam S, et al. 2022. J Immunol. 208:1341. PubMed
  24. Uhlig K, et al. 2015. J Virol. 89: 9044-9060. PubMed
  25. Christian LS, et al. 2021. Cell Reports. 35(6):109118. PubMed
  26. Wang D, et al. 2018. Immunity. 48:659. PubMed
  27. Baptista AP et al. 2019. Immunity. 50(5):1188-1201 . PubMed
  28. Israelow B, et al. 2021. Sci Immunol. 6:eabl4509. PubMed
  29. McLane LM, et al. 2021. Cell Reports. 35(6):109120. PubMed
  30. Xiong A, et al. 2022. EBioMedicine. 83:104239. PubMed
  31. Chung H, et al. 2021. Immune Netw. 21:e28. PubMed
  32. Sugimoto C, et al. 2022. Elife. 11:. PubMed
  33. Bartleson JM, et al. 2020. Nat Immunol. 1384:21. PubMed
  34. Silva M, et al. 2021. Sci Immunol. 6:eabf1152. PubMed
  35. Müller M, et al. 2021. Nat Commun. 12:7036. PubMed
  36. Ni P, et al. 2014. J Immunol . 193:1778. PubMed
  37. Yu Y, et al. 2022. Nat Commun. 13:6357. PubMed
  38. Samarchith P Kurup et al. 2019. Cell host & microbe. 25(4):565-577 . PubMed
  39. Nelson SA, et al. 2022. NPJ Vaccines. 7:124. PubMed
  40. Levescot A, et al. 2021. J Clin Invest. 131:. PubMed
  41. Yin Q, et al. 2021. Proc Natl Acad Sci U S A. 118: . PubMed
  42. Flommersfeld S, et al. 2021. Immunity. :. PubMed
  43. Cuburu N, et al. 2019. J Immunol. 202:1250. PubMed
  44. Imani J, et al. 2021. JCI Insight. 6:. PubMed
  45. Reed J, et al. 2020. Bio Protoc. 10:e3607. PubMed
  46. Pitsch J, et al. 2021. Ann Neurol. 89:666. PubMed
  47. Nayak RR, et al. 2021. Cell Host Microbe. 29(3):362-377.e11. PubMed
  48. Ouyang W, et al. 2021. Invest Ophthalmol Vis Sci. 62:25:00. PubMed
  49. Becher J, et al. 2018. Dev Cell. 47:592. PubMed
  50. Yadava K et al. 2019. Elife. 8 pii: e44821. PubMed
  51. Toomer K, et al. 2016. J Immunol. 196: 3665 - 3676. PubMed
  52. Liu Y, et al. 2017. Oncogene. 10.1038/onc.2017.209. PubMed
  53. Van Braeckel-Budimir N, et al. 2018. Cell Rep. 24:3374. PubMed
  54. Frost JN, et al. 2021. Med (N Y). 2:164. PubMed
  55. Simula L et al. 2018. Cell reports. 25(11):3059-3073 . PubMed
  56. Karandikar SH, et al. 2019. Anal Chem. 91:3405. PubMed
  57. Toshiro Hirai et al. 2019. Immunity. 50(5):1249-1261 . PubMed
  58. Mendelsohn AC, et al. 2020. Am J Physiol Cell Physiol. 319:C997. PubMed
  59. Zhang C, et al. 2021. J Immunother Cancer. 9:. PubMed
  60. Dholakia J, et al. 2022. Gynecol Oncol. 164:170. PubMed
  61. Isvoranu G, et al. 2019. Oncol Lett. 17:4197. PubMed
  62. Tsalavos S, et al. 2011. J Immunol. 186:6860. PubMed
  63. Liu Y, et al. 2020. Cell Death Dis. 11:1062. PubMed
  64. Israelow B, et al. 2020. J Exp Med. 217:00:00. PubMed
  65. Mairhofer D, et al. 2015. J Invest Dermatol. 135: 2785-93. PubMed
  66. Clemente–Casares X, et al. 2017. Immunity. 47:974. PubMed
  67. Chao JL, et al. 2021. Cell Rep Med. 2:100399. PubMed
  68. Di Pilato M, et al. 2021. Cell. 184(17):4512-4530.e22. PubMed
  69. Theurich S et al. 2017. Cell metabolism. 26(1):171-184 . PubMed
  70. Malenica I, et al. 2021. Nat Commun. 12:5209. PubMed
  71. Ikeda T, et al. 2014. PLoS One. 9:115198. PubMed
  72. Qi H, et al. 2020. Cell Reports. 31(6):107621. PubMed
  73. Guo Y, et al. 2021. Nat Immunol. 22:746. PubMed
  74. Agelidis A, et al. 2017. Cell Rep. 10.1016/j.celrep.2017.06.041. PubMed
  75. Caetano MS, et al. 2019. Clin Cancer Res. 25:7576. PubMed
  76. Yang F, et al. 2021. Nat Commun. 12:3424. PubMed
  77. Pan Y, et al. 2021. NPJ Vaccines. 6:1. PubMed
  78. Chow AK, et al. 2021. Cellular and Molecular Gastroenterology and Hepatology. :. PubMed
  79. Gonzalez-Figueroa P, et al. 2021. Cell. 184(7):1775-1789.e19. PubMed
  80. Qi S, et al. 2020. Theranostics. 10:1814. PubMed
  81. Riopel M, et al. 2019. Mol Metab. 20:89. PubMed
RRID
AB_493564 (BioLegend Cat. No. 104511)
AB_493564 (BioLegend Cat. No. 104512)

Antigen Details

Structure
C-type lectin, 27/33 kD
Distribution

Activated T cells and B cells, NK cells, granulocytes, thymocytes, platelets

Function
Lymphocyte activation
Cell Type
B cells, Granulocytes, NK cells, Platelets, T cells, Thymocytes, Tregs
Biology Area
Costimulatory Molecules, Immunology, Innate Immunity
Molecular Family
CD Molecules
Antigen References

1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Testi R, et al. 1994. Immunol. Today 15:479.
3. Moretta A, et al. 1991. J. Exp. Med. 174:1393.
4. Yokoyama WM, et al. 1988. J. Immunol. 141:369.

Gene ID
12515 View all products for this Gene ID
UniProt
View information about CD69 on UniProt.org
Go To Top Version: 3    Revision Date: 04.18.2016

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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