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PE/Cyanine7 anti-mouse/human CD282 (TLR2) Recombinant Antibody

Pricing & Availability
Clone
QA16A01 (See other available formats)
Regulatory Status
RUO
Other Names
Toll-like receptor 2, TLR-2, TIL4, CD282, TLR2
Isotype
Mouse IgG1, κ
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Product Citations
publications
QA16A01_PECy7_CD282_TLR2_RECOM_Antibody_062921.png
Human peripheral blood cells were stained with PE/Cyanine7 anti-mouse/human CD282 (TLR2) recombinant (clone QA16A01) (filled histogram) or isotype control mouse IgG1, κ PE/Cyanine7 (open histogram).
  • QA16A01_PECy7_CD282_TLR2_RECOM_Antibody_062921.png
    Human peripheral blood cells were stained with PE/Cyanine7 anti-mouse/human CD282 (TLR2) recombinant (clone QA16A01) (filled histogram) or isotype control mouse IgG1, κ PE/Cyanine7 (open histogram).
Compare all formats See PE/Cyanine7 spectral data
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153011 25 µg 141€
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153012 100 µg 352€
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Description

CD282 (Toll-like receptor 2, TLR2) is an 85kDa, type I transmembrane protein, with 14 leucine-rich repeats (LRR) in the extracellular domain and a toll/IL-1R (TIR) in the cytoplasmic region. TLR2 is expressed by monocytes/macrophages, dendritic cells, granulocytes, endothelial cell and epithelial cells. TLR2 associates with TLR1 and TLR6 in the cell surface and is involved in the recognition of pathogen-associated molecular patterns (PAMPs) such as peptidoglycan, lipoteihoic acid (LTA), lipoarabinomanan, lipoproteins, and LPS from certain Gram-negative bacteria, yeast, spirochete and fungi.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse, Human
Antibody Type
Recombinant
Host Species
Mouse
Immunogen
Recombinant extracellular domain of murine TLR2
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with PE/Cyanine7 under optimal conditions.
Concentration
0.2 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Blocking of IL-6 and TNF-a production induced by P3CSK4 on RAW264.7 cells was observed during development.

RRID
AB_2894693 (BioLegend Cat. No. 153011)
AB_2894693 (BioLegend Cat. No. 153012)

Antigen Details

Structure
Type I transmembrane protein, 14 leucine-rich repeats (LRR) in the extracellular domain, the intracellular domain contains a toll/IL-1R (TIR) domain.
Distribution

Monocytes/macrophages, dendritic cells, granulocytes, endothelial cell, epithelial cells.

Function
Activates innate host defense, induces production of pro-inflammatory cytokines.
Interaction
TLR1, TLR6 , MYD88 and TRAF6.
Ligand/Receptor
Peptidoglycan, lipoteihoic acid (LTA), lipoarabinomanan, lipoproteins, as well as different LPS from certain Gram-negative bacteria, yeast, spirochete and fungi.
Cell Type
Dendritic cells, Endothelial cells, Epithelial cells, Granulocytes, Macrophages, Monocytes, Neutrophils
Biology Area
Cell Biology, Immunology, Innate Immunity, Signal Transduction
Molecular Family
CD Molecules, Toll Like Receptors
Antigen References

1. Biswas S, et al. 2016. Blood. 127:2618.
2. Wang Y, et al. 2016. Proc. Natl. Acad. Sci. USA 113:884.
3. Ramstead AG, et al. 2016. Infect. Immun. 84:940.
4. Scheeren FA, et al. 2014. Nat. Cell. Biol. 16:1238.
5. Barbalat R, et al. 2009. Nat. Immunol. 10:1200. 

Gene ID
24088 View all products for this Gene ID 7097 View all products for this Gene ID
UniProt
View information about CD282 on UniProt.org
Go To Top Version: 1    Revision Date: 06.30.2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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