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PE/Cyanine7 anti-mouse Ly-6G/Ly-6C (Gr-1) Antibody

Pricing & Availability
Clone
RB6-8C5 (See other available formats)
Regulatory Status
RUO
Other Names
Gr-1
Isotype
Rat IgG2b, κ
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Product Citations
publications
RB6-8C5_PECy7_040912
C57BL/6 mouse bone marrow cells were stained with Ly-6G/Ly-6C (clone RB6-8C5) PE/Cyanine7 (filled histogram) or rat IgG2b, κ PE/Cyanine7 isotype control (open histogram). Data shown was gated on myeloid cell population.
  • RB6-8C5_PECy7_040912
    C57BL/6 mouse bone marrow cells were stained with Ly-6G/Ly-6C (clone RB6-8C5) PE/Cyanine7 (filled histogram) or rat IgG2b, κ PE/Cyanine7 isotype control (open histogram). Data shown was gated on myeloid cell population.
Compare all formats See PE/Cyanine7 spectral data
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108415 25 µg 71€
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108416 100 µg 152€
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Description

Gr-1 is a 21-25 kD protein also known as Ly-6G/Ly-6C. This myeloid differentiation antigen is a glycosylphosphatidylinositol (GPI)-linked protein expressed on granulocytes and macrophages. In bone marrow, the expression levels of Gr-1 directly correlate with granulocyte differentiation and maturation; Gr-1 is also transiently expressed on bone marrow cells in the monocyte lineage. Immature Myeloid Gr-1+ cells play a role in the development of antitumor immunity.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Raised against granulocytes of mouse origin
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE/Cyanine7 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Clone RB6-8C5 binds with high affinity to mouse Ly-6G molecules and to a lower extent to Ly-6C19. Clone RB6-8C5 impairs the binding of anti-mouse Ly-6G clone 1A819. However, clone RB6-8C5 is able to stain in the presence of anti-mouse Ly-6C clone HK1.420.

The RB6-8C5 antibody has been used to identify peripheral blood neutrophils and deplete granulocytes in vivo. Additional reported applications (for relevant formats of this clone) include: in vitro complement-mediated cytotoxicity2, in vivo depletion3-5,9, immunoprecipitation1, immunohistochemical staining6 (including paraffin-embedded sections9,16,33-35, acetone-fixed frozen sections11 and zinc-fixed sections15), and Western blotting7. RB6-8C5 is not suitable for depletion of hepatic myeloid derived suppressor cells (MDSCs)20.

Special Note: For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 108436).

Application References
  1. Fleming TJ, et al. 1993. J. Immunol. 151:2399. (IP)
  2. Brummer E, et al. 1984. J. Leukocyte Biol. 36:505. (CMCD)
  3. Stoppacciaro A, et al. 1993. J. Exp. Med. 178:151. (Deplete)
  4. Tumpey TM, et al. 1996. J. Virol. 70:898. (Deplete)
  5. Czuprynski CJ, et al. 1994. J. Immunol. 152:1836. (Deplete)
  6. Nitta H, et al. 1997. Cell Vision 4:73. (IHC)
  7. Jutila MA, et al. 1988. Eur. J. Immunol. 18:1819. (WB)
  8. Engwerda CR, et al. 2004. Am. J. Pathol. 165:2123.
  9. Brown CR, et al. 2004. Infect. Immun. 72:4956. (Deplete, IHC)
  10. Andoniou CE, et al. 2005. Nature Immunology 6:1011. (FC) PubMed
  11. Li M, et al. 2006. P. Natl. Acad. Sci USA 103:11736. (IHC)
  12. Dzhagalov I, et al. 2007. Blood 109:1620. (FC) PubMed
  13. Fazilleau N, et al. 2007. Nature Immunol. 8:753. (FC) PubMed
  14. Heuser M, et al. 2007. Blood 110:1639. (FC) PubMed
  15. Wang T, et al. 2007. Infect. Immun. 75:1144. (IHC)
  16. Bosio CM, et al. 2007. J. Immunol. 178:4538. (IHC)
  17. Boehme SA, et al. 2009. Int. Immunol. 21:81. (IHC)
  18. Piao Y, et al. 2012. Neuro Oncol. 14:1379. PubMed
  19. Ribechini E, et al. 2009. Eur. J. Immunol. 39:3538.
  20. Ma C, et al. 2012. J. Leukoc. Biol. 92:1199.
  21. Li J, et al. 2012. Arthritis Rheum. 64:1098. PubMed
  22. Fan Q, et al. 2014. Cancer Res. 74:471. PubMed
  23. Korrer MJ, et al. 2014. PLoS One. 9:91370. PubMed
  24. Morshed M, et al. 2014. J Immunol. 192:5314. PubMed
  25. Collins C, et al. 2014. PNAS. 111:9899. PubMed
  26. Madireddi S, et al. 2014. J Exp Med. 211:1433. PubMed
  27. Bianchi G, et al. 2014. Cell Death Dis. 5:1135. PubMed
  28. Guo H, et al. 2014. J Leukoc Biol. 96:419. PubMed
  29. Roderick JE, et al. 2014. PNAS. 111:14436. PubMed
  30. Distel E, et al. 2014. Circ Res. 115:759. PubMed
  31. Iwai H, et al. 2015. Tuberculosis. 95:246. PubMed
  32. Charmsaz S, et al. 2015. PLoS One. 10:130692. PubMed
  33. Whiteland J, et al. 1994 J Histochem Cytochem 43:3 (IHC-P)
  34. Brown C, et al. 2003 J Immunology 171:2 (IHC-P)
  35. Obregon-Henao A, et al. PLoS One 8:11 (IHC-P)
Product Citations
  1. Katsumura KR, et al. 2018. Proc Natl Acad Sci U S A. 115:E10109. PubMed
  2. Tran NT, et al. 2019. Cell Rep. 28:3510. PubMed
  3. Montagner S, et al. 2016. Cell Rep. 15: 1566-1579. PubMed
  4. Bussières-Marmen S, et al. 2017. Cell Mol Immunol. 10.1038/cmi.2016.72. PubMed
  5. Lawson H, et al. 2021. Stem Cell Reports. 16:2784. PubMed
  6. Casellas Roman HL, et al. 2020. Leuk Res. 94:106372. PubMed
  7. Nagamachi A, et al. 2021. J Clin Invest. 131:. PubMed
  8. Aimaitijiang A, et al. 2022. Genes Cells. 27:25. PubMed
  9. Wang B, et al. 2022. EMBO J. 41:e108946. PubMed
  10. Chae CS, et al. 2022. Cancer Discov. 12:1904. PubMed
  11. Georgiev GI, et al. 2022. Cell Chem Biol. 29:811. PubMed
  12. Schönberger K, et al. 2023. Anal Chem. 95:4325. PubMed
  13. Liu PS, et al. 2023. Nat Immunol. 24:452. PubMed
  14. Li G, et al. 2023. Cell Death Dis. 14:185. PubMed
  15. McNamara ME, et al. 2023. JCI Insight. . PubMed
  16. Mawambo G, et al. 2023. J Neuroinflammation. 20:145. PubMed
  17. Schönberger K, et al. 2022. Cell Stem Cell. 29:131. PubMed
  18. Han X, et al. 2017. Int J Mol Sci. 10.3390/ijms18050942. PubMed
  19. Bieren J, et al. 2015. J Immunol. 194:1154. PubMed
  20. Paris J et al. 2019. Cell Stem Cell. 25(1):137-148 . PubMed
  21. Hirata Y et al. 2018. Cell stem cell. 22(3):445-453 . PubMed
  22. Yu K, et al. 2021. Cell Reports. 35(12):109273. PubMed
  23. Gritsina G, et al. 2015. Mol Cancer Ther. 14:1035. PubMed
  24. Zhao B, et al. 2021. Cell Reports. 36(3):109401. PubMed
  25. Wang Y, et al. 2022. Front Immunol. 13:767630. PubMed
  26. Stahlhut M, et al. 2021. Sci Rep. 11:5294. PubMed
  27. Bhattacharya P, et al. 2022. PLoS Negl Trop Dis. 16:e0010224. PubMed
  28. Gordon RA, et al. 2020. PLoS One. 15:e0226396. PubMed
  29. Jakob L, et al. 2021. Int J Mol Sci. 22:. PubMed
  30. Kotaki R, et al. 2020. Sci Rep. 10:13554. PubMed
  31. Bignami F, et al. 2017. Acta Ophthalmol. 95:e641. PubMed
  32. Li Y, et al. 2020. Cell Stem Cell. 27(5):732-747.e7. PubMed
  33. Sá da Bandeira D, et al. 2022. Cell Rep. 40:111114. PubMed
  34. Yang P, et al. 2022. Nat Commun. 13:5782. PubMed
  35. Davis M, et al. 2015. J Immunol. 194:2219. PubMed
  36. Tran NT, et al. 2020. STAR Protocols. 1(1):100028. PubMed
  37. Fu Z, et al. 2021. Cell Res. 31:631. PubMed
  38. Dagher T, et al. 2021. J Exp Med. 218:. PubMed
  39. Madsen P, et al. 2016. J Cereb Blood Flow Metab. 36: 1553 - 1569. PubMed
  40. Mehta C, et al. 2021. Nucleic Acids Res. 49:9007. PubMed
  41. Brusilovsky M, et al. 2021. Nat Immunol. 22:1316. PubMed
  42. Zhang CR, et al. 2022. Blood Cancer Discov. 3:220. PubMed
  43. Wang S, et al. 2021. Commun Biol. 22:. PubMed
  44. Chen R, et al. 2021. Cell Reports. 34(7):108751. PubMed
  45. Draijer C, et al. 2018. Sci Rep. 8:5105. PubMed
  46. Celik H, et al. 2018. Cancer Cell. 34:741. PubMed
  47. Cencic R, et al. 2009. PLoS One. 4:e5223. PubMed
  48. Varikuti S, et al. 2020. Br J Cancer. 122:1005. PubMed
  49. Da Mesquita S, et al. 2021. Science Advances. 7(21):. PubMed
  50. Zhang MS, et al. 2022. Nat Commun. 13:954. PubMed
  51. Schönberger K, et al. 2022. STAR Protoc. 3:101408. PubMed
  52. Valkenburg S, et al. 2016. Sci Rep. 6:22666. PubMed
  53. Xie X, et al. 2015. Sci Rep. 5: 18115. PubMed
  54. Sanders K, et al. 2015. Cancer Immunol Res. 3: 891-901. PubMed
  55. Hoffmann O, et al. 2015. Infect Immun . 83:259. PubMed
  56. Erin N, et al. 2015. Brain Behav Immun. 48: 174-185. PubMed
  57. Magee JA, et al. 2021. Stem Cell Reports. 16:20. PubMed
  58. Schnoegl D, et al. 2022. Front Immunol. 13:909270. PubMed
  59. Manríquez V, et al. 2021. Nat Commun. 12:4547. PubMed
  60. Riedel SS, et al. 2021. Molecular Cell. 81(11):2332-2348.e9. PubMed
  61. Li K, et al. 2020. Nat Commun. 0.795138889. PubMed
  62. Hidalgo San Jose L, et al. 2020. Cell Rep. 30:69. PubMed
  63. Hartwig T et al. 2017. Molecular cell. 65(4):730-742 . PubMed
  64. Luo H, et al. 2019. Cell Rep. 26:945. PubMed
  65. Peltzer N, et al. 2018. Nature. 557:112. PubMed
  66. Li A, et al. 2018. Cancer Lett. 431:54. PubMed
  67. Xu G, et al. 2015. Free Radic Biol Med. 87: 15-25. PubMed
  68. Phadke MS, et al. 2021. Cancer Immunol Res. 9:554. PubMed
  69. Gu Z, et al. 2021. Nat Genet. 53:672. PubMed
  70. Yang F, et al. 2021. Nat Commun. 12:3424. PubMed
  71. Badgujar DC, et al. 2020. PLoS Pathog. 16:e1009016. PubMed
  72. Syed I et al. 2018. Cell metabolism. 27(2):419-427 . PubMed
RRID
AB_313380 (BioLegend Cat. No. 108415)
AB_313380 (BioLegend Cat. No. 108416)

Antigen Details

Structure
21-25 kD
Distribution

Granulocytes, monocytes

Cell Type
Granulocytes, Monocytes, Neutrophils
Biology Area
Immunology, Innate Immunity
Antigen References

1. Fleming TJ, et al. 1993. J. Immunol. 151:2399.
2. Jutila MA, et al. 1988. Eur. J. Immunol. 18:1819.
3. Goni O, et al. 2002. Int. Immunol. 14:1125.

Gene ID
17067 View all products for this Gene ID 546644 View all products for this Gene ID
UniProt
View information about Ly-6G Ly-6C on UniProt.org

Related FAQs

There are no FAQs for this product.

Other Formats

View All Ly-6G/Ly-6C Reagents Request Custom Conjugation
Description Clone Applications
APC anti-mouse Ly-6G/Ly-6C (Gr-1) RB6-8C5 FC
Biotin anti-mouse Ly-6G/Ly-6C (Gr-1) RB6-8C5 FC,IHC,IP,WB
FITC anti-mouse Ly-6G/Ly-6C (Gr-1) RB6-8C5 FC
PE anti-mouse Ly-6G/Ly-6C (Gr-1) RB6-8C5 FC
PE/Cyanine5 anti-mouse Ly-6G/Ly-6C (Gr-1) RB6-8C5 FC
Purified anti-mouse Ly-6G/Ly-6C (Gr-1) RB6-8C5 FC,IHC,IP,WB
PE/Cyanine7 anti-mouse Ly-6G/Ly-6C (Gr-1) RB6-8C5 FC
Alexa Fluor® 488 anti-mouse Ly-6G/Ly-6C (Gr-1) RB6-8C5 FC,IHC
Alexa Fluor® 647 anti-mouse Ly-6G/Ly-6C (Gr-1) RB6-8C5 FC,SB
Alexa Fluor® 700 anti-mouse Ly-6G/Ly-6C (Gr-1) RB6-8C5 FC
Brilliant Violet 711™ anti-mouse Ly-6G/Ly-6C (Gr-1) RB6-8C5 FC
APC/Cyanine7 anti-mouse Ly-6G/Ly-6C (Gr-1) RB6-8C5 FC
Pacific Blue™ anti-mouse Ly-6G/Ly-6C (Gr-1) RB6-8C5 FC
PerCP/Cyanine5.5 anti-mouse Ly-6G/Ly-6C (Gr-1) RB6-8C5 FC
PerCP anti-mouse Ly-6G/Ly-6C (Gr-1) RB6-8C5 FC
Brilliant Violet 421™ anti-mouse Ly-6G/Ly-6C (Gr-1) RB6-8C5 FC
Brilliant Violet 570™ anti-mouse Ly-6G/Ly-6C (Gr-1) RB6-8C5 FC
Ultra-LEAF™ Purified anti-mouse Ly-6G/Ly-6C (Gr-1) RB6-8C5 FC,IP,CMCD,Depletion,IHC,WB
Brilliant Violet 510™ anti-mouse Ly-6G/Ly-6C (Gr-1) RB6-8C5 FC
Brilliant Violet 605™ anti-mouse Ly-6G/Ly-6C (Gr-1) RB6-8C5 FC
Brilliant Violet 650™ anti-mouse Ly-6G/Ly-6C (Gr-1) RB6-8C5 FC
Alexa Fluor® 594 anti-mouse Ly-6G/Ly-6C (Gr-1) RB6-8C5 IHC-F,FC
Purified anti-mouse Ly-6G/Ly-6C (Gr-1) (Maxpar® Ready) RB6-8C5 FC,CyTOF®
PE/Dazzle™ 594 anti-mouse Ly-6G/Ly-6C (Gr-1) RB6-8C5 FC
APC/Fire™ 750 anti-mouse Ly-6G/Ly-6C (Gr-1) RB6-8C5 FC
TotalSeq™-A0116 anti-mouse Ly-6G/Ly-6C (Gr-1) RB6-8C5 PG
TotalSeq™-C0116 anti-mouse Ly-6G/Ly-6C (Gr-1) RB6-8C5 PG
TotalSeq™-B0116 anti-mouse Ly-6G/Ly-6C (Gr-1) RB6-8C5 PG
Spark Blue™ 550 anti-mouse Ly-6G/Ly-6C (Gr-1) RB6-8C5 FC
APC/Fire™ 810 anti-mouse Ly-6G/Ly-6C (Gr-1) RB6-8C5 FC
Spark Violet™ 423 anti-mouse Ly-6G/Ly-6C (GR-1) Antibody RB6-8C5 FC
Spark UV™ 387 anti-mouse Ly-6G/Ly-6C (GR-1) RB6-8C5 FC
Spark Violet™ 538 anti-mouse Ly-6G/Ly-6C (Gr-1) RB6-8C5 FC
Spark PLUS UV395™ anti-mouse Ly-6G/Ly-6C (Gr-1) RB6-8C5 FC
Spark Red™ 718 anti-mouse Ly-6G/Ly-6C (Gr-1) (Flexi-Fluor™) RB6-8C5 FC
Spark Blue™ 574 anti-mouse Ly-6G/Ly-6C (Gr-1) (Flexi-Fluor™) RB6-8C5 FC
Go To Top Version: 2    Revision Date: 12.09.2013

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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