PE/Cyanine7 anti-rat CD4 Antibody anti-CD4

Pricing & Availability

Clone: W3/25 (See other available formats)
Regulatory Status: RUO
Other Names: T4, L3T4, W3/25
Isotype: Mouse IgG1, κ
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Product Citations: publications

LOU rat splenocytes stained with W3/25 PE/Cyanine7

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201515

25 µg

118€

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201516

100 µg

259€

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Description

CD4 is a 55 kD glycoprotein also known as T4. Rat CD4 is a member of the immunoglobulin superfamily and is expressed on majority of thymocytes, macrophages, and a peripheral T cell subset (T helper cells). CD4 is a T cell co-receptor that interacts with the MHC class II molecule and is involved in T cell activation.

Product Details

Technical Data Sheet (pdf)

Product Details

Verified Reactivity: Rat
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Rat thymocyte membrane glycoproteins
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with PE/Cyanine7 under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: The W3/25 antibody has been shown to inhibit IL-2 production by T helper cells and to prevent autoimmune T cell transfer in an MBP induced EAE model in vivo. Additional reported applications (for the relevant formats) include: immunohistochemistry of acetone-fixed frozen sections^1,2, inhibition of IL-2 production³, inhibition of MBP-induced T cell activation in EAE transfer model³.
RRID: AB_1186087 (BioLegend Cat. No. 201515)
AB_1186087 (BioLegend Cat. No. 201516)

Antigen Details

Structure: Ig superfamily, 55 kD
Distribution: Majority of thymocytes, macrophages, and a peripheral T cell subset (T helper cells)
Function: T cell co-receptor, T cell activation
Ligand/Receptor: MHC class II molecule
Cell Type: Macrophages, T cells, Thymocytes
Biology Area: Immunology
Molecular Family: CD Molecules
Antigen References: 1. Brideau RJ, et al. 1980. Eur. J. Immunol. 10:609.
2. Clark SJ, et al. 187. P. Natl. Acad. Sci. USA 84:1649.
Gene ID: 24936 View all products for this Gene ID 24937 View all products for this Gene ID
UniProt: View information about CD4 on UniProt.org

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Related Protocols

Cell Surface Flow Cytometry Staining Protocol

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Other Formats

View All CD4 Reagents Request Custom Conjugation

Description	Clone	Applications
Purified anti-rat CD4	W3/25	FC,IHC-F
FITC anti-rat CD4	W3/25	FC
PE anti-rat CD4	W3/25	FC
APC anti-rat CD4	W3/25	FC
Alexa Fluor® 488 anti-rat CD4	W3/25	FC,IHC-F
APC/Cyanine7 anti-rat CD4	W3/25	FC
PE/Cyanine7 anti-rat CD4	W3/25	FC
PerCP/Cyanine5.5 anti-rat CD4	W3/25	FC

Certificate of Analysis

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Go To Top Version: 1 Revision Date: 11.30.2012

For Research Use Only. Not for diagnostic or therapeutic use.

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