PE/Fire™ 810 anti-mouse CD279 (PD-1) Antibody, CD279, 29F.1A12

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Clone: 29F.1A12 (See other available formats)
Regulatory Status: RUO
Other Names: PD-1, Programmed Death-1, PDCD1
Isotype: Rat IgG2a, κ
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Product Citations: publications

29Fdot1A12_PEFire810_CD279_Antibody_121421 — Con-A and IL-2 stimulated C57BL/6 mouse splenocytes (three days) were stained with anti-mouse CD3 APC and anti-mouse CD279 (PD-1) (clone 29F.1A12) PE/Fire ™ 810 (left) or CD3 APC only.

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135253

25 µg

137€

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Description

CD279, also known as programmed death-1 (PD-1), is a 50-55 kD glycoprotein belonging to the CD28 family of the Ig superfamily. PD-1 is expressed on activated splenic T and B cells and thymocytes. It is induced on activated myeloid cells as well. PD-1 is involved in lymphocyte clonal selection and peripheral tolerance through binding its ligands, B7-H1 (PD-L1) and B7-DC (PD-L2). It has been reported that PD-1 and PD-L1 interactions are critical to positive selection and play a role in shaping the T cell repertoire. PD-L1 negative costimulation is essential for prolonged survival of intratesticular islet allografts.

Product Details

Technical Data Sheet (pdf)

Product Details

Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: PD-1 cDNA followed by PD-1-Ig fusion protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography and conjugated with PE/Fire™ 810 under optimal conditions.
Concentration: 0.2 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

* PE/Fire™ 810 has a maximum excitation of 488/561 nm and a maximum emission of 810 nm.

Excessive exposure to light, and commonly used fixation, permeabilization buffers can affect PE/Fire™ 810 fluorescence signal intensity and spread. Please keep conjugates protected from light exposure. For more information and representative data, visit our Fire Dyes page.
Excitation Laser: Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue³, in vivo blocking of PD-1 binding to its ligands^2,3, and spatial biology (IBEX)^5,6.
RRID: AB_2910293 (BioLegend Cat. No. 135253)

Antigen Details

Structure: A 50-55 kD glycoprotein belonging to the CD28 family of the Ig superfamily.
Distribution: Induced on splenic T and B lymphocytes, thymocytes, and myeloid cells after stimulation.
Function: Involved in lymphocyte clonal selection and peripheral tolerance, prolonged survival of allografts.
Ligand/Receptor: B7-H1 (PD-L1) and B7-DC (PD-L2)
Cell Type: B cells, T cells
Biology Area: Cancer Biomarkers, Immunology, Inhibitory Molecules
Molecular Family: CD Molecules, Immune Checkpoint Receptors
Antigen References: 1. Nishimura H, et al. 2001. Science 291:319
2. Agata Y, et al. 1996. Int. Immunol. 8:765
3. Liang SC, et al. 2003. Eur. J. Immunol. 33:2706
4. Barber DL, et al. 2006. Nature 439:682
5. Keir ME, et al. 2005. J. Immunol. 175:7372
6. Koehn BH. et al. 2008. J Immunol. 181:5313
Gene ID: 18566 View all products for this Gene ID
UniProt: View information about CD279 on UniProt.org

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Cell Surface Flow Cytometry Staining Protocol

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Other Formats

View All CD279 Reagents Request Custom Conjugation

Description	Clone	Applications
PE anti-mouse CD279 (PD-1)	29F.1A12	FC
Purified anti-mouse CD279 (PD-1)	29F.1A12	FC,IHC-F,Block,ELISA
PerCP/Cyanine5.5 anti-mouse CD279 (PD-1)	29F.1A12	FC
APC anti-mouse CD279 (PD-1)	29F.1A12	FC
Biotin anti-mouse CD279 (PD-1)	29F.1A12	FC
FITC anti-mouse CD279 (PD-1)	29F.1A12	FC
PE/Cyanine7 anti-mouse CD279 (PD-1)	29F.1A12	FC
Brilliant Violet 421™ anti-mouse CD279 (PD-1)	29F.1A12	FC,SB
Brilliant Violet 605™ anti-mouse CD279 (PD-1)	29F.1A12	FC
APC/Cyanine7 anti-mouse CD279 (PD-1)	29F.1A12	FC
Brilliant Violet 785™ anti-mouse CD279 (PD-1)	29F.1A12	FC
PE/Dazzle™ 594 anti-mouse CD279 (PD-1)	29F.1A12	FC
Alexa Fluor® 647 anti-mouse CD279 (PD-1)	29F.1A12	FC
Brilliant Violet 711™ anti-mouse CD279 (PD-1)	29F.1A12	FC
GoInVivo™ Purified anti-mouse CD279 (PD-1)	29F.1A12	FC
APC/Fire™ 750 anti-mouse CD279 (PD-1)	29F.1A12	FC
Brilliant Violet 510™ anti-mouse CD279 (PD-1)	29F.1A12	FC
Ultra-LEAF™ Purified anti-mouse CD279 (PD-1)	29F.1A12	FC,IHC-F,Block
APC/Fire™ 810 anti-mouse CD279 (PD-1) Antibody	29F.1A12	FC
PE/Fire™ 810 anti-mouse CD279 (PD-1) Antibody	29F.1A12	FC
PE/Cyanine5 anti-mouse CD279 (PD-1)	29F.1A12	FC
PE/Fire™ 640 anti-mouse CD279 (PD-1)	29F.1A12	FC
Spark Red™ 718 anti-mouse CD279 (PD-1)	29F.1A12	FC
PerCP/Fire™ 806 anti-mouse CD279 (PD-1)	29F.1A12	FC
Brilliant Violet 750™ anti-mouse CD279 (PD-1)	29F.1A12	FC
PerCP/Fire™ 780 anti-mouse CD279 (PD-1)	29F.1A12	FC
PE/Fire™ 700 anti-mouse CD279 (PD-1)	29F.1A12	FC
Brilliant Violet 650™ anti-mouse CD279 (PD-1)	29F.1A12	FC
Spark Blue™ 574 anti-mouse CD279 (PD-1) (Flexi-Fluor™)	29F.1A12	FC
Spark PLUS B550™ anti-mouse CD279 (PD-1)	29F.1A12	FC

Certificate of Analysis

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Go To Top Version: 2 Revision Date: 05.03.2023

For Research Use Only. Not for diagnostic or therapeutic use.

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