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PerCP/Cyanine5.5 anti-human Arginase 1 Antibody

Pricing & Availability
Clone
14D2C43 (See other available formats)
Regulatory Status
RUO
Other Names
ARG1, Liver Arginase, Type 1 Arginase, Arginase-1, Liver-Type Arginase
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
14D2C43_PERCP_CYANINE55_Arginase-I_Antibody_012421
Human peripheral blood was surface stained with anti-human CD15 APC and anti-human CD16 Pacific Blue™, fixed, permeabilized, and then intracellularly stained with anti-human Arginase I (clone 14D2C43) PerCP/Cyanine5.5 (filled histogram) or mouse IgG2b, κ PerCP/Cyanine5.5 isotype control (open histogram). Histogram was gated on the neutrophil (CD15+CD16+) population.
  • 14D2C43_PERCP_CYANINE55_Arginase-I_Antibody_012421
    Human peripheral blood was surface stained with anti-human CD15 APC and anti-human CD16 Pacific Blue™, fixed, permeabilized, and then intracellularly stained with anti-human Arginase I (clone 14D2C43) PerCP/Cyanine5.5 (filled histogram) or mouse IgG2b, κ PerCP/Cyanine5.5 isotype control (open histogram). Histogram was gated on the neutrophil (CD15+CD16+) population.
Compare all formats See PerCP/Cyanine5.5 spectral data
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369709 25 tests 151€
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369710 100 tests 329€
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Description

Arginase I, also known as ARG1, is a 34.7kD protein expressed by neutrophils and myeloid-derived suppressor cells (MDSCs). There are two isoforms that are differentiated based on their tissue distribution and subcellular localization. Arginase I converts L-arginine into L-ornithine and urea; it is the final enzyme in the urea cycle. While mostly found in the liver, it can also be expressed in cells lacking a comprehensive urea cycle. Also, it contributes to vasodilation and vascular function. Arginase I is also reported to be involved in MDSC mediated suppression of T cell proliferation.  Arginase I has been implicated in hyperargininemia (decreased function of arginase I) and Q fever.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Full length recombinant protein expressed in E.coli.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC – Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm. 

Excitation Laser
Blue Laser (488 nm)
RRID
AB_2910417 (BioLegend Cat. No. 369709)
AB_2910417 (BioLegend Cat. No. 369710)

Antigen Details

Structure
Belongs to the ureohydrolase family and has a molecular mass of 34.7 kD.
Distribution
Neutrophils and myeloid-derived suppressor cells (MDSC).
Function
Converts L-arginine into L-ornithine and urea.
Interaction
Arginine.
Cell Type
Neutrophils
Biology Area
Cell Biology, Immunology, Neuroinflammation, Neuroscience
Molecular Family
Enzymes and Regulators
Antigen References

1. Munder M, et al. 2005. Blood 105:2549.
2. Luckner-Minden C, et al. 2010. JLB. 87:1125.
3. Holowatz L, et al. 2006. Journal of Physiology 574:573.
4. Sin YY, et al. 2013. PLoS One 8:11.
5. Benoit M, et al. 2008. Eur. J. Immunol. 4:1065.

Gene ID
383 View all products for this Gene ID
UniProt
View information about Arginase I on UniProt.org

Related FAQs

How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?

PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.

Go To Top Version: 1    Revision Date: 01.24.2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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