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- Clone
- hec2 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- CD99, MIC2, HBA71, MSK5X, E2 antigen
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
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Human peripheral blood lymphocytes were stained with anti-human CD99 (clone hec2) PerCP/Cyanine5.5 (filled histogram) or mouse IgG1, κ PerCP/Cyanine5.5 isotype control (open histogram).
| Cat # | Size | Price | Quantity Check Availability | Save | ||
|---|---|---|---|---|---|---|
| 398209 | 25 tests | 141€ | ||||
| 398210 | 100 tests | 306€ | ||||
CD99 is a type I single chain transmembrane protein devoid of N-linked glycosylation sites encoded by the pseudoautosomal gene MIC2. CD99 has an apparent molecular weight of 32 kD and is widely expressed on a variety of tissues. CD99 is highly expressed on thymocytes, T cells, and T cell leukemias and lymphomas. However, it is absent on some B cell lines, fetal B cells, eosinophils, granulocytes and the NK-cell line YT. CD99 is involved in spontaneous rosette formation with erythrocytes and may also be involved in other T-cell and hematopoietic cell adhesion pathways. CD99 has been reported to activate a caspase-independent death pathway in T cells under some conditions. CD99 interacts with a number of proteins including ferritin heavy chain 1, karyopherin beta 1, TRIP13, cyclophilin A, annexin II, and ubiquitin-conjugating enzyme E2H.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Human endothelial cells
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm. - Application References
-
- Schenkel AR, et al. 2002. Nat. Immunol. 3:143-50. (IHC, FC, WB)
- Lou O, et al. 2007. J. Immunol. 178:1136-43. (Block, FC)
- Watson RL, et al. 2015. J. Exp. Med. 212:1021-41. (IP, WB)
- RRID
-
AB_2832778 (BioLegend Cat. No. 398209)
AB_2832778 (BioLegend Cat. No. 398210)
Antigen Details
- Structure
- Surface glycoprotein, type I transmembrane protein, 32 kD.
- Distribution
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T-cells, thymocytes, T-cell leukemias and lymphomas. It is absent on fetal B-cells, eosinophils, granulocytes, and NK cell line YT.
- Function
- Leukocyte migration, T-cell adhesion, caspase independent T-cell death, and spontaneous rosette formation with erythrocytes.
- Interaction
- Annexin II, E2H, Ferritin heavy chain 1, karyopherin beta 1, and TRIP13.
- Cell Type
- B cells, Basophils, Dendritic cells, Fibroblasts, Macrophages, Mast cells, NK cells, Platelets, T cells
- Biology Area
- Immunology
- Molecular Family
- CD Molecules
- Antigen References
-
- Gelin C, et al. 1989. EMBO J. 8:3253-59.
- Goodfellow PJ, et al. 1986. Science. 234:740-43.
- Pettersen RD, et al. 2001. J. Immunol. 166:4931-42.
- Gene ID
- 4267 View all products for this Gene ID
- UniProt
- View information about CD99 on UniProt.org
Related Pages & Pathways
Pathways
Related FAQs
- How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?
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PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.
Other Formats
View All CD99 Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-human CD99 | hec2 | FC,Block,IHC,IP,WB |
| PerCP/Cyanine5.5 anti-human CD99 | hec2 | FC |
| FITC anti-human CD99 | hec2 | FC |
| PE anti-human CD99 | hec2 | FC |
| APC anti-human CD99 | hec2 | FC |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-human CD99
Human peripheral blood lymphocytes were stained with purifie... -
PerCP/Cyanine5.5 anti-human CD99
Human peripheral blood lymphocytes were stained with anti-hu... -
FITC anti-human CD99
Human peripheral blood lymphocytes were stained with anti-hu... -
PE anti-human CD99
Human peripheral blood lymphocytes were stained with anti-hu... -
APC anti-human CD99
Human peripheral blood lymphocytes were stained with anti-hu...
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