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PerCP/Cyanine5.5 anti-human CD99 Antibody

Pricing & Availability
Clone
hec2 (See other available formats)
Regulatory Status
RUO
Other Names
CD99, MIC2, HBA71, MSK5X, E2 antigen
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
hec2_PerCPCyanine55_CD99_Antibody_012820
Human peripheral blood lymphocytes were stained with anti-human CD99 (clone hec2) PerCP/Cyanine5.5 (filled histogram) or mouse IgG1, κ PerCP/Cyanine5.5 isotype control (open histogram).
  • hec2_PerCPCyanine55_CD99_Antibody_012820
    Human peripheral blood lymphocytes were stained with anti-human CD99 (clone hec2) PerCP/Cyanine5.5 (filled histogram) or mouse IgG1, κ PerCP/Cyanine5.5 isotype control (open histogram).
Compare all formats See PerCP/Cyanine5.5 spectral data
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398209 25 tests 141€
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398210 100 tests 306€
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Description

CD99 is a type I single chain transmembrane protein devoid of N-linked glycosylation sites encoded by the pseudoautosomal gene MIC2. CD99 has an apparent molecular weight of 32 kD and is widely expressed on a variety of tissues. CD99 is highly expressed on thymocytes, T cells, and T cell leukemias and lymphomas. However, it is absent on some B cell lines, fetal B cells, eosinophils, granulocytes and the NK-cell line YT. CD99 is involved in spontaneous rosette formation with erythrocytes and may also be involved in other T-cell and hematopoietic cell adhesion pathways. CD99 has been reported to activate a caspase-independent death pathway in T cells under some conditions. CD99 interacts with a number of proteins including ferritin heavy chain 1, karyopherin beta 1, TRIP13, cyclophilin A, annexin II, and ubiquitin-conjugating enzyme E2H.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human endothelial cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

Application References
  1. Schenkel AR, et al. 2002. Nat. Immunol. 3:143-50. (IHC, FC, WB)
  2. Lou O, et al. 2007. J. Immunol. 178:1136-43. (Block, FC)
  3. Watson RL, et al. 2015. J. Exp. Med. 212:1021-41. (IP, WB)
RRID
AB_2832778 (BioLegend Cat. No. 398209)
AB_2832778 (BioLegend Cat. No. 398210)

Antigen Details

Structure
Surface glycoprotein, type I transmembrane protein, 32 kD.
Distribution

T-cells, thymocytes, T-cell leukemias and lymphomas. It is absent on fetal B-cells, eosinophils, granulocytes, and NK cell line YT.

Function
Leukocyte migration, T-cell adhesion, caspase independent T-cell death, and spontaneous rosette formation with erythrocytes.
Interaction
Annexin II, E2H, Ferritin heavy chain 1, karyopherin beta 1, and TRIP13.
Cell Type
B cells, Basophils, Dendritic cells, Fibroblasts, Macrophages, Mast cells, NK cells, Platelets, T cells
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References
  1. Gelin C, et al. 1989. EMBO J. 8:3253-59. 
  2. Goodfellow PJ, et al. 1986. Science. 234:740-43. 
  3. Pettersen RD, et al. 2001. J. Immunol. 166:4931-42.
Gene ID
4267 View all products for this Gene ID
UniProt
View information about CD99 on UniProt.org

Related FAQs

How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?

PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.

Go To Top Version: 1    Revision Date: 01.28.2020

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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