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PerCP/Cyanine5.5 anti-human TCR Vγ9 Antibody

Pricing & Availability
Clone
B3 (See other available formats)
Regulatory Status
RUO
Other Names
T cell receptor Vγ9, TCR Vγ2 (Strauss, Quertermous nomenclature), TRGV9, TCRGV2S1
Isotype
Mouse IgG1, κ
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Product Citations
publications
B3_PerCPCyanine55_TCR_Vgamma9_Antibody_081619
Human peripheral blood lymphocytes were stained with CD3 (clone UCHT1) PE and anti-human TCR Vκ9 (clone B3) PerCP/Cyanine5.5 (left) or mouse IgG1, κ PerCP/Cyanine5.5 isotype control (right).
  • B3_PerCPCyanine55_TCR_Vgamma9_Antibody_081619
    Human peripheral blood lymphocytes were stained with CD3 (clone UCHT1) PE and anti-human TCR Vκ9 (clone B3) PerCP/Cyanine5.5 (left) or mouse IgG1, κ PerCP/Cyanine5.5 isotype control (right).
Compare all formats See PerCP/Cyanine5.5 spectral data
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331321 25 tests 141€
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331322 100 tests 329€
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Description

The Vγ9 TCR is a variant of the TCR γ chain expressed on a subset of γ/δ T cells. Vγ9Vδ2 T lymphocytes, a major γ/δ T cell subset in humans, recognize phosphoantigens, certain tumor cells, and cells treated with aminobisphosphonates. This cell population displays cytolytic activity against various tumor cells. The γ/δ TCR is a heterodimeric TCR complex composed of covalently bound γ and δ chains involved in antigen recognition and the non-covalently associated monomorphic proteins CD3δ, γ, ε, and ζ chains.

The B3 antibody reacts specifically with human TCR Vγ9 as designated by the Lefranc/Foster nomenclature system. Human TCR Vγ9 is also known as TCR Vγ2 under the Strauss, Quertermous nomenclature system.

Several TCR γ and δ chain nomenclature systems exist. In order to consolidate the various nomenclature systems, we have created charts for corresponding names across some of the most popular naming methods. Click here to see charts.
 

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Reported Reactivity
Chimpanzee, Cynomolgus
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

Excitation Laser
Blue Laser (488 nm)
Application References
  1. Van Rhijn I, et al. 2003. Intl. Immunol. 15:373.
  2. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
RRID
AB_2814210 (BioLegend Cat. No. 331321)
AB_2814210 (BioLegend Cat. No. 331322)

Antigen Details

Structure
Ig superfamily member, TCR γ/δ with CD3 forms the CD3/TCR complex
Distribution

Expressed on majority of peripheral γ/δ T cells

Function
Antigen recognition, T cell activation
Ligand/Receptor
Peptide bound to MHC
Cell Type
T cells
Biology Area
Adaptive Immunity, Immunology
Molecular Family
TCRs
Antigen References
  1. Scotet E, et al. 2005. Immunity 22:71
  2. Rincon-Orozco B, et al. 2005. J. Immunol. 175:2144
Gene ID
6965 View all products for this Gene ID
UniProt
View information about TCR Vgamma9 on UniProt.org

Related FAQs

How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?

PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.

Go To Top Version: 1    Revision Date: 08.16.2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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