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PerCP/Cyanine5.5 anti-mouse CD134 (OX-40) Antibody

Pricing & Availability
Clone
OX-86 (See other available formats)
Regulatory Status
RUO
Other Names
TNFRSF4, ACT35, OX-40
Isotype
Rat IgG1, κ
Ave. Rating
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Product Citations
publications
OX-86_PerCPCyanine5dot5_CD134_Antibody_020819_updated.png
Con-A + IL-2 stimulated (3 days) C57BL/6 splenocytes were stained with CD134 (clone OX-86) PerCP/Cyanine5.5 (closed histogram) or Rat IgG1, κ PerCP/Cyanine5.5 isotype control (open histogram).
  • OX-86_PerCPCyanine5dot5_CD134_Antibody_020819_updated.png
    Con-A + IL-2 stimulated (3 days) C57BL/6 splenocytes were stained with CD134 (clone OX-86) PerCP/Cyanine5.5 (closed histogram) or Rat IgG1, κ PerCP/Cyanine5.5 isotype control (open histogram).
Compare all formats See PerCP/Cyanine5.5 spectral data
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119424 25 µg 137€
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119425 100 µg 329€
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Description

CD134 is a type I integral membrane protein also known as OX-40, ACT35, and tumor necrosis factor receptor superfamily member 4 (TNFRSF4). This receptor is expressed on activated CD4+ and CD8+ T cells and B cells. The OX-40 receptor binds to the OX-40 ligand (CD252) to provide a costimulatory signal that is independent of CD28. Blockade of OX40-OX40 ligand interactions has been shown to ameliorate experimental EAE and inflammatory bowel disease, which implies that these interactions are important in the pathogenesis of some autoimmune diseases.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant mouse OX-40-CD4 chimeric protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is = 1 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. 

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

Excitation Laser
Blue Laser (488 nm)
Application Notes

Clone OX-86 has been reported to act as an agonist and stimulate OX-40.

Application References
  1. Higgins LM, et al. 1999. J. Immunol. 162:486. (FC, IHC)
  2. Al-Shamkhani A, et al. 1996. Eur. J. Immunol. 26:1695. (Costim)
  3. del Rio ML, et al. 2011. Transpl. Int. 24:501. (FC) PubMed
Product Citations
  1. Fedele C, et al. 2021. J Exp Med. 218: . PubMed
RRID
AB_2715994 (BioLegend Cat. No. 119424)
AB_2715994 (BioLegend Cat. No. 119425)

Antigen Details

Structure
TNF receptor superfamily, 50 kD
Distribution

Activated CD4+ and CD8+ T cells, activated B cells.

Function
Receptor for OX-40 ligand, provides co-stimulatory signal for lymphocyte proliferation independent of CD28; thought to play a role in the pathogenesis of some autoimmune diseases
Ligand/Receptor
OX-40 ligand
Cell Type
B cells, T cells, Tregs
Biology Area
Immunology
Molecular Family
CD Molecules, Immune Checkpoint Receptors
Antigen References

1. Al-Shamkhani A, et al. 1996. Eur. J. Immunol. 26:1695.
2. Weinberg AD, et al. 1999. J. Immunol. 162:1818.
3. Akira H, et al. 1999. J. Immunol. 162:7058.
4. Pippig SD, et al. 1999. J. Immunol. 163:6520.
5. Higgins LM, et al. 1999. J. Immunol. 162:486.

Gene ID
22163 View all products for this Gene ID
UniProt
View information about CD134 on UniProt.org

Related FAQs

How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?

PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.

Go To Top Version: 2    Revision Date: 02.08.2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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