PerCP/Fire™ 806 anti-human CD183 (CXCR3) Antibody, CD183, G025H7

Pricing & Availability

Clone: G025H7 (See other available formats)
Regulatory Status: RUO
Other Names: CXCR3, G protein-coupled receptor 9 (GPR9), CKR-L2
Isotype: Mouse IgG1, κ
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Product Citations: publications

G025H7_-PerCP-Fire-806_CD183_FC_100924 — Human peripheral blood lymphocytes were stained with anti-human CD3 (clone UCHT1) Brilliant Violet 421™ and anti-human CD183 (CXCR3) (clone G025H7) PerCP/Fire™ 806 (left) or mouse IgG1, κ PerCP/Fire™ 806 isotype control (right).

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353771

25 tests

160€

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353772

100 tests

368€

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Description

Human CXCR3, also known as GPR9, is a chemokine receptor that binds CXCL9, CXCL10, and CXCL11. It is a 38 kD seven-pass transmembrane receptor coupled to G-protein. CXCR3 is highly expressed by T cells (Th1), natural killer cells (NK cells), dendritic cells, mast cells, alveolar macrophages, eosinophils, and human airway epithelial cells. CXCR3 is important for effector lymphocyte recruitment into inflamed tissue in various inflammatory and autoimmune diseases, such as chronically inflamed liver, Crohn's disease, rheumatoid arthritis, multiple sclerosis, and inflammatory skin diseases.

Product Details

Technical Data Sheet (pdf)

Product Details

Verified Reactivity: Human, Cynomolgus, Rhesus
Reported Reactivity: African Green, Baboon
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Human CXCR3 transfectants
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with PerCP/Fire™ 806 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

* PerCP/Fire™ 806 has a maximum excitation of 478 nm and a maximum emission of 806 nm.
Excitation Laser: Blue Laser (488 nm)

Antigen Details

Structure: CXC-chemokine receptor, G protein-coupled receptor, seven-pass transmembrane receptor
Distribution: T cell subset, NK cells, plasmacytoid dendritic cells, GM-CSF activated CD34⁺ hematopoietic progenitors, mast cells, alveolar macrophages, eosinophils, and airway epithelial cells
Function: Essential in T cell recruitment to sites of inflammation
Ligand/Receptor: CXCL9, CXCL10, and CXCL11
Cell Type: Dendritic cells, Eosinophils, Epithelial cells, Hematopoietic stem and progenitors, Macrophages, Mast cells, NK cells, T cells, Tregs
Biology Area: Cell Biology, Immunology, Neuroinflammation, Neuroscience
Molecular Family: CD Molecules, Cytokine/Chemokine Receptors, GPCR
Antigen References: 1. Loetscher M, et al. 1996. J. Exp. Med. 184:963.
2. Cole KE, et al. 1998. J. Exp. Med. 187:2009.
3. Aksoy MO, et al. 2006. Am. J. Physiol. Lung Cell Mol. Physiol. 290:L909.
4. Curbishley SM, et al. 2005. Am. J. Pathol. 167:887.
5. Turner JE, et al. 2007. Mini. Rev. Med. Chem. 7:1089.
6. Wenzel J, et al. 2008. J. Invest. Dermatol. 128:67.
Gene ID: 2833 View all products for this Gene ID
UniProt: View information about CD183 on UniProt.org

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Related Protocols

Cell Surface Flow Cytometry Staining Protocol

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Pathways

Related FAQs

Does staining at room temperature or even at 37°C help for checking chemokine receptors expression?: Due to continuous recycling of many chemokine receptors, it may be worthwhile to consider staining at room temperature or at 37°C if the staining at lower temperature (which can potentially reduce receptor turnover) is not optimal.

Other Formats

View All CD183 Reagents Request Custom Conjugation

Description	Clone	Applications
Purified anti-human CD183 (CXCR3)	G025H7	FC
APC/Cyanine7 anti-human CD183 (CXCR3)	G025H7	FC
FITC anti-human CD183 (CXCR3)	G025H7	FC
PE anti-human CD183 (CXCR3)	G025H7	FC
APC anti-human CD183 (CXCR3)	G025H7	FC
Alexa Fluor® 488 anti-human CD183 (CXCR3)	G025H7	FC
Alexa Fluor® 647 anti-human CD183 (CXCR3)	G025H7	FC
PerCP/Cyanine5.5 anti-human CD183 (CXCR3)	G025H7	FC
Brilliant Violet 421™ anti-human CD183 (CXCR3)	G025H7	FC
PE/Cyanine7 anti-human CD183 (CXCR3)	G025H7	FC
Pacific Blue™ anti-human CD183 (CXCR3)	G025H7	FC
Brilliant Violet 510™ anti-human CD183 (CXCR3)	G025H7	FC
Brilliant Violet 605™ anti-human CD183 (CXCR3)	G025H7	FC
Brilliant Violet 650™ anti-human CD183 (CXCR3)	G025H7	FC
Brilliant Violet 711™ anti-human CD183 (CXCR3)	G025H7	FC
Purified anti-human CD183 (CXCR3) (Maxpar® Ready)	G025H7	FC,CyTOF®
PE/Dazzle™ 594 anti-human CD183 (CXCR3)	G025H7	FC
PerCP anti-human CD183 (CXCR3)	G025H7	FC
Brilliant Violet 785™ anti-human CD183 (CXCR3)	G025H7	FC
Alexa Fluor® 700 anti-human CD183 (CXCR3)	G025H7	FC
Biotin anti-human CD183 (CXCR3)	G025H7	FC
TotalSeq™-A0140 anti-human CD183 (CXCR3)	G025H7	PG
TotalSeq™-C0140 anti-human CD183 (CXCR3)	G025H7	PG
Ultra-LEAF™ Purified anti-human CD183 (CXCR3)	G025H7	Block,FC
TotalSeq™-B0140 anti-human CD183 (CXCR3)	G025H7	PG
APC/Fire™ 750 anti-human CD183 (CXCR3)	G025H7	FC
TotalSeq™-D0140 anti-human CD183 (CXCR3)	G025H7	PG
PE/Fire™ 810 anti-human CD183 (CXCR3) Antibody	G025H7	FC
APC/Fire™ 810 anti-human CD183 (CXCR3) Antibody	G025H7	FC
PE/Cyanine5 anti-human CD183 (CXCR3) Antibody	G025H7	FC
PE/Fire™ 640 anti-human CD183 (CXCR3)	G025H7	FC
PerCP/Fire™ 780 anti-human CD183 (CXCR3)	G025H7	FC
Spark Red™ 718 anti-human CD183 (CXCR3) (Flexi-Fluor™)	G025H7	FC
PE/Fire™ 700 anti-human CD183 (CXCR3)	G025H7	FC
PE anti-human CD183	G025H7	FC

Certificate of Analysis

Go To Top Version: 1 Revision Date: 10.09.2024

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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