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PhosphoPair STAT3 (Tyr705) Antibody Set

Pricing & Availability
Clone
4G4B45; 13A3-1;
Regulatory Status
RUO
Other Names
Signal Transducer And Activator Of Transcription 3, Acute-Phase Response Factor, APRF
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Product Citations
publications
PhosphoPair_STAT3_Antibody_Set_08112
Whole cell extracts (15 µg protein) from serum starved A-431 cells untreated (-) or treated (+) with 100 ng/mL of EGF for 5 minutes were resolved on a 4-12% Bis-Tris gel and transferred to a nitrocellulose membrane. Membranes were probed with 0.5 µg/mL (1:1000 dilution) of purified anti-STAT3 Phospho (Tyr705) antibody (clone 13A3-1) for 2 hours at room temperature. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG antibody (Cat. No. 405306) at a 1:3000 dilution. Purified anti-STAT3 antibody (clone 4G4B45) was used as a pan STAT3 loading control at 1.0 µg/mL (1:500 dilution). Lane M: Molecular weight marker.
  • PhosphoPair_STAT3_Antibody_Set_08112
    Whole cell extracts (15 µg protein) from serum starved A-431 cells untreated (-) or treated (+) with 100 ng/mL of EGF for 5 minutes were resolved on a 4-12% Bis-Tris gel and transferred to a nitrocellulose membrane. Membranes were probed with 0.5 µg/mL (1:1000 dilution) of purified anti-STAT3 Phospho (Tyr705) antibody (clone 13A3-1) for 2 hours at room temperature. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG antibody (Cat. No. 405306) at a 1:3000 dilution. Purified anti-STAT3 antibody (clone 4G4B45) was used as a pan STAT3 loading control at 1.0 µg/mL (1:500 dilution). Lane M: Molecular weight marker.
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Description

The STAT (Signal Transducer and Activator of Transcription) proteins represent a family of transcription factors that function as downstream effectors of growth factor and cytokine signaling cascades. STAT3 plays a central role in cell cycle progression, inhibition of apoptosis, and transformation. Phosphorylation of STAT3 at tyrosine 705 by JAK and receptor tyrosine kinases has been shown to increase STAT3 activation through multiple mechanisms, including increased homodimerization and concomitant translocation to the nucleus, as well as through increasing STAT3 affinity for response elements found in the regulatory promoters of its target genes.  High levels of STAT3 activation have been described in several cancers and are of considerable interest for therapeutic intervention.

Product Details
Technical Data Sheet (pdf)

Kit Contents

Kit Contents
Product Application Clone Size Reactivity Isotype
Purified anti-STAT3 Antibody WB, ICFC, ICC, IP 4G4B45 25 μg Human, Mouse Mouse IgG1, κ
Purified anti-STAT3 Phospho (Tyr705) Antibody WB, ICC 13A3-1 25 μg Human, Mouse Mouse IgG1, κ


* For detailed information about each specificity, please refer to the datasheets of the individual products. 

Product Details

Verified Reactivity
Human, Mouse
Immunogen
Please refer to individual product datasheets for immunogen information
Formulation
Phosphate-buffered solutions, pH 7.2, containing 0.09% sodium azide
Preparation
The antibodies were purified by affinity chromatography.
Storage & Handling
The antibody solutions should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
Recommended Usage

Each lot of antibodies in this set is quality control tested by Western blotting. For Western blotting, the suggested uses of these reagents are as follows:

Clone 4G4B45: 0.25 - 1.0 µg/mL
Clone 13A3-1: 0.5 - 1.0 µg/mL

It is recommended that the reagent be titrated for optimal performance for each application.

For recommended usage information for additional applications, please refer to the datasheet of the individual products.

Antigen Details

Structure
STAT3 exists as two isoforms: STAT3α is a 770 amino acid protein with a predicted molecular weight of ~88 kD, STAT3β is a 722 amino acid protein with a predicted molecular weight of ~83 kD.
Distribution

Ubiquitously expressed/Nucleus and cytosol

Function
Cell signaling, transcription factor
Antigen References

1. Akira S, et al. 1994. Cell 77:63.
2. Zhang X, et al. 1995. Science 267:1990.
3. Sanchez-Margalet V and Martin-Romero C. 2001. Cell. Immunol. 211:30.
4. Simon A, et al. 2000. Science 290:144.
5. Hoey T and Grusby MJ. 1999. Adv. Immunol. 71:145.

Gene ID
6774 View all products for this Gene ID
UniProt
View information about STAT3 on UniProt.org

Related FAQs

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Go To Top Version: 1    Revision Date: 08.11.2020

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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