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Purified anti-ALDH1A1 Antibody

Pricing & Availability
Clone
M562 (See other available formats)
Regulatory Status
RUO
Other Names
Aldehyde Dehydrogenase 1 Family Member A1, Retinaldehyde Dehydrogenase, ALDH-E1, RALDH 1, ALHDII, ALDH1, PUMB1, ALDC, Epididymis Secretory Sperm Binding Protein Li 53e
Isotype
Mouse IgG2a, κ
Ave. Rating
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Product Citations
publications
M562_Purified_ALDH1A1_Antibody_1_031819
Western blot of purified anti-ALDH1A1 antibody (clone M562). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain lysate. The blot was incubated with 1.0 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence (Cat. No. 426302) was used as the detection system.
  • M562_Purified_ALDH1A1_Antibody_1_031819
    Western blot of purified anti-ALDH1A1 antibody (clone M562). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain lysate. The blot was incubated with 1.0 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence (Cat. No. 426302) was used as the detection system.
  • M562_Purified_ALDH1A1_Antibody_2_031819
    IHC staining of purified anti-ALDH1A1 antibody (clone M562) on formalin-fixed paraffin-embedded human cerebral cortex tissue. Following antigen retrieval using Citrate H.I.E.R (Cat. No. 420902), the tissue was incubated with 0.5 µg/mL of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided.
  • M562_Purified_ALDH1A1_Antibody_3_070620
    IHC staining of purified anti-ALDH1A1 (clone M562) on formalin-fixed paraffin-embedded human brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R (Cat. No. 928602), the tissue was incubated with 5 µg/mL of the primary antibody overnight at 4°C, followed by incubation with 2.5 µg/mL of Alexa Fluor® 594 goat anti-mouse IgG for one hour at room temperature. The slide was mounted with Fluoromount-G™, with DAPI. The image was captured with a 40X objective. Scale Bar: 50 µm
  • M562_Purified_ALDH1A1_Antibody_4_070620
    ICC staining of purified anti-ALDH1A1 (clone M562) on A549 cell. The cells were fixed with 4% PFA, permeabilized with a buffer containing 0.1% Triton X-100 and 0.25% BSA, and blocked with 2% normal goat serum and 0.02% BSA. The cells were then incubated with 2 µg/mL of the primary antibody overnight at 4°C, followed by incubation with 2.5 µg/mL of Alexa Fluor® 594 goat anti-mouse IgG for one hour at room temperature. The cells were co-stained with Flash Phalloidin™ Green 488 (Cat. No. 424201). The slide was mounted with fluoromount G with DAPI. The image was captured with a 60X objective. Scale bar: 20 µm
Cat # Size Price Quantity Check Availability Save
861901 25 µg 85€
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861902 100 µg 221€
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Description

ALDH1A1 belongs to the aldehyde dehydrogenase family of proteins and is involved in the metabolism of alcohol. There are two major liver isoforms, mitochondrial and cystolic, which are involved in the alcohol metabolism pathway. ALDH1A1 is also involved in retinol metabolism where retinol is converted to retinoic acid. Hematopoietic stem cell differentiation is suggested to be mediated through ALDH1A1 control of the retinoid signaling pathway.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Clone M562 was generated from a recombinant protein corresponding to amino acids in the N-terminal region from human ALDH1A1.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
IHC-P, ICC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1.0 - 5.0 µg per mL. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 0.5 - 5.0 µg/mL for chromogenic staining and 2.0 - 5.0 µg/mL for fluorescent staining is suggested. For immunocytochemistry, a concentration range of 2.0 - 10 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2801235 (BioLegend Cat. No. 861901)
AB_2801235 (BioLegend Cat. No. 861902)

Antigen Details

Structure
ALDH1A1 is a 501 amino acid protein with a molecular mass of ~55 kD.
Distribution

Tissue Distribution: Liver, kidney, red blood cells, skeletal muscle, lung, breast, lens, stomach, brain, pancreas, testis, prostate, and ovary.
Cellular Distribution: Extracellular, cytosol, cytoskeleton, mitochondria, and nucleus.

Function
ALDH1A1 binds free retinal and cellular retinol-binding protein-bound retinal. ALDH1A1 is also responsible for the oxidation retinaldehyde to retinoic acid.
Cell Type
Endothelial cells, Oligodendrocytes
Biology Area
Neuroscience
Molecular Family
Aldehyde Dehydrogenases
Antigen References
  1. Xu SL, et al. Am J Cancer Res. 2015 Mar 15;5(4):1471-83.
  2. Olmez I, et al. J Cell Mol Med. 2015 Jun;19(6):1262-72.
  3. Schäfer A, et al. Neuro Oncol. 2012 Dec;14(12):1452-64.
  4. Adam SA, et al. Brain Pathol. 2012 Nov;22(6):788-97.
Gene ID
216 View all products for this Gene ID
UniProt
View information about ALDH1A1 on UniProt.org
Go To Top Version: 2    Revision Date: 07.06.2020

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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