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Purified anti-ATG17 Antibody

Pricing & Availability
Clone
W16188A (See other available formats)
Regulatory Status
RUO
Other Names
RB1-inducible coiled-coil protein 1, FAK family kinase-interacting protein of 200 Da,FIP200, ATG17, KIAA0203
Isotype
Rat IgG2a, κ
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Product Citations
publications
W16188A_PURE_ATG17_Antibody_WB_1_091417
Total lysates (15 µg protein) from HeLa (Lane 1), 10nM scrambled siRNA (lane 2), 1nM RB1CC1 siRNA (Lane 3) and 10nM RB1CC1 siRNA treated HeLa cells (Lane 4), 293T (Lane 5), 10nM scrambled siRNA (lane 6), 1nM RB1CC1 siRNA (Lane 7) and 10nM RB1CC1 siRNA treated 293T cells (Lane 8) were resolved by electrophoresis, transferred to nitrocellulose, and probed with 1:500 (1 µg/mL) diluted purified anti-RB1CC1 antibody (clone W16188A). Proteins were visualized by chemiluminescence detection using 1:3000 diluted HRP rat-IgG secondary antibody conjugated to HRP. 1:2000 dilution of Direct-Blot™ HRP anti-β-actin antibody (clone 2F1-1) was used as a loading control (lower). Lane M: Molecular weight ladder.
  • W16188A_PURE_ATG17_Antibody_WB_1_091417
    Total lysates (15 µg protein) from HeLa (Lane 1), 10nM scrambled siRNA (lane 2), 1nM RB1CC1 siRNA (Lane 3) and 10nM RB1CC1 siRNA treated HeLa cells (Lane 4), 293T (Lane 5), 10nM scrambled siRNA (lane 6), 1nM RB1CC1 siRNA (Lane 7) and 10nM RB1CC1 siRNA treated 293T cells (Lane 8) were resolved by electrophoresis, transferred to nitrocellulose, and probed with 1:500 (1 µg/mL) diluted purified anti-RB1CC1 antibody (clone W16188A). Proteins were visualized by chemiluminescence detection using 1:3000 diluted HRP rat-IgG secondary antibody conjugated to HRP. 1:2000 dilution of Direct-Blot™ HRP anti-β-actin antibody (clone 2F1-1) was used as a loading control (lower). Lane M: Molecular weight ladder.
  • W16188A_PURE_ATG17_Antibody_WB_2_091417
    Total cell lysate (15 µg protein) from NIH3T3, B16F10 and A20 cells were resolved by electrophoresis (4-12% Bis-Tris gel), transferred to nitrocellulose, and probed with 1:500 (1 µg/mL) diluted purified anti-RB1CC1 antibody (clone W16188A) or competitor’s antibody used at four-fold manufactures recommended concentration. Proteins were visualized by chemiluminescence detection using 1:3000 diluted HRP rat-IgG secondary antibody conjugated to HRP for clone W16188A or 1:3000 diluted donkey anti-rabbit IgG Antibody conjugated to HRP for competitor’s antibody (upper). 1:2000 dilution of Direct-Blot™ HRP anti-β-actin antibody (clone 2F1-1) was used as a loading control (lower). Lane M: Molecular weight ladder.
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620001 25 µg 81€
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620002 100 µg 203€
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Description

RB1-inducible coiled-coil 1 (RB1CC1), also known as ATG17 or FIP200, was originally identified as a potent regulator of RB1 gene. RB1CC1 is a component of the ULK1-ATG13-RB1CC1 complex, which is required for the early events of autophagy and is inhibited by mTOR. RB1CC1 has been suggested to participate in the NF-kβ, mTOR, and FAK-Erk1/2 signaling pathways and regulates cell proliferation, apoptosis, and migration. RB1CC1 enhances transcription of RB1, p16, and p21 when forms a complex with p53, PIASy, and hSNF5. RB1CC1 plays an important role in tumor suppression by globally activation of the RB1 pathway. Aberrant expression or genetic alterations of RB1CC1 has been associates with various disease, including cancer, neuronal degeneration, skin inflammation, and anemia. 

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant human RB1CC1 (1345-1594 a.a.) expressed in E. coli.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.2 - 2.0 µg per ml (1:250-1:2500 dilution). It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This clone does not work for IF microscopy application (in-house tested).

RRID
AB_2716038 (BioLegend Cat. No. 620001)
AB_2716038 (BioLegend Cat. No. 620002)

Antigen Details

Structure
1594 amino acids with a predicted molecular weight of 183 kD. Contains two C-terminal coiled coil domains.
Distribution

Nucleus, Cytoplasm. 

Function
RB1CC1 is a mammalian homolog of yeast ATG17 and is involved in the regulation of cell growth, proliferation, apoptosis, migration, and autophagy.
Interaction
RB1CC1 interacts with ULK1, ATG13, FAK1, PYK2, p53, RFWD2, GABARAPL1, GABARAP, ATG16L1, RNF111, SKI, and SMAD7.
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Neuroscience, Neuroscience Cell Markers, Transcription Factors
Molecular Family
Autophagosome Markers, Nuclear Markers
Antigen References

1. Zhang LY, et al. 2016. Carcinogenesis. 37:320.
2. Yao J, et al. 2015. Autophagy. 11:939.
3. Suraneni MV, et al. 2014. Cell Cycle. 13:1798.
4. Morselli E, et al. 2011. Cell Cycle. 10:2763.
5. Ikebuchi K, et al. 2009. Int. J. Cancer. 125:861.
6. Chano T, et al. 2002. Nat Genet. 31:285. 

Gene ID
9821 View all products for this Gene ID
UniProt
View information about ATG17 on UniProt.org
Go To Top Version: 0    Revision Date: 09.18.2017

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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