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Purified anti-B-Raf Antibody

Pricing & Availability
Clone
W21141A (See other available formats)
Regulatory Status
RUO
Other Names
BRAF, V-Raf Murine Sarcoma Viral Oncogene Homolog B1, Serine/threonine-protein kinase B-raf, Proto-oncogene B-Raf, p94, Proto-oncogene B-Raf, BRAF1, BRAF-1, RAFB1
Isotype
Rat IgG2b, κ
Ave. Rating
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Product Citations
publications
W21141A_PURE_B-Raf_Antibody_1_010824
Whole cell extracts (15 µg total protein) from indicated cell lines were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 0.5 µg/mL of Purified anti-B-Raf (clone W21141A) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP Goat anti-rat IgG (Cat. No. 405405). Direct-Blot™ HRP anti-GAPDH (Cat. No. 607904) was used as a loading control at a 1:50000 dilution. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Lane M: Molecular weight marker
  • W21141A_PURE_B-Raf_Antibody_1_010824
    Whole cell extracts (15 µg total protein) from indicated cell lines were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 0.5 µg/mL of Purified anti-B-Raf (clone W21141A) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP Goat anti-rat IgG (Cat. No. 405405). Direct-Blot™ HRP anti-GAPDH (Cat. No. 607904) was used as a loading control at a 1:50000 dilution. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Lane M: Molecular weight marker
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625051 25 µg 144€
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625052 100 µg 356€
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Description

B-Raf is one of three proteins in the RAF family of serine/threonine protein kinases. B-Raf regulates the mitogen-activated protein kinase/extracellular signal-regulated kinase (MAPK/ERK) pathway. Like the other two RAF kinases, A-Raf and C-Raf, B-Raf relays extracellular signals to the nucleus through the MAPK/ERK pathway and regulates cell division, differentiation, and secretion. RAF proteins share similar protein domains and motifs, including multiple protein binding domains such as Ras, lipid, and the 14-3-3 binding motif. RAF proteins mainly reside in the cytoplasm in an inactive state by binding to 14-3-3 proteins. RAFs translocate to the membrane compartment by activation and binding with RAS proteins. Similar to other RAF kinases, B-Raf phosphorylates MAP2K1 (MEK) proteins to activate the MAPK/ERK pathway. Mutations in the gene which encodes B-Raf are highly correlated with inherited cardiofaciocautaneous syndrome and various types of cancers. The B-Raf V600E mutation results in a constitutively active kinase leading to aberrant cell proliferation and invasion. Such mutations are linked to hairy cell leukemia, melanoma, colon cancer, and non-small-cell lung cancer. B-Raf inhibitors are considered as anticancer targets for metastatic melanoma, kidney cancer, and liver cancer.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant fragment of human B-Raf
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.1 - 1.0 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This product cross-reacts with mouse in western blotting (WB).

This product is not recommended for immunocytochemistry (ICC).

RRID
AB_3097499 (BioLegend Cat. No. 625051)
AB_3097499 (BioLegend Cat. No. 625052)

Antigen Details

Structure
It forms homodimers and heterodimers with C-Raf. It binds to 14-3-3, Ras, or MAP2K1.
Distribution

Cytoplasm, membrane, and nucleus

Function
Phosphorylates MAP2K1 and activates the MAPK/ERK pathway
Interaction
Regulates the MAPK and ERK pathways; interacts with PFKFB2
Biology Area
Cancer Biomarkers, Cardiovascular Biology, Cell Cycle/DNA Replication, Cell Proliferation and Viability, Neuroscience, Synaptic Biology
Molecular Family
Postsynaptic proteins, Phospho-Proteins, Protein Kinases/Phosphatase
Antigen References
  1. Davies H, et al. 2002. Nature. 17(6892):949-54.
  2. Wajapeyee N, et al. 2008. Cell. 132:363-74.
  3. Tiacci E, et al. 2011. N Engl J Med. 364:2305-15.
  4. Dankner M, et al. 2018. Oncogene. 37:3183-3199.
Gene ID
673 View all products for this Gene ID
UniProt
View information about B-Raf on UniProt.org
Go To Top Version: 1    Revision Date: 01.08.2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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