- Clone
- C219 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Multidrug resistance protein 3, P-glycoprotein 3, multiple drug resistance 3, ATP-binding cassette sub-family B member 4, P glycoprotein 3/multiple drug resistance 3
- Previously
-
Signet Catalog# 8710-01
Covance Catalog# SIG-38710
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
Cat # | Size | Price | Quantity Check Availability | Save | ||
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903701 | 1 mL | 484€ |
Clone C219 recognizes an internal, highly conserved amino acid sequence (VQEALD & VQAALD) found in both MDR1 and MDR3 isoforms of P-glycoprotein. The specific binding of the clone C219 was stronger in epitope sequence VQAALD (N-Terminal fragment) than the epitope sequence VQEALD (C-terminal fragmant) (Georges E., et al., 1990). Analysis of p-glycoprotein expression in skeletal and cardiac muscle revealed that C219 cross reacts with a 200kDa protein that migrates in the same position as myosin. C219 has also been reported to detect the 185kDa c-erbB2 protein (Liu, et al., 1997). Care should be taken in the interpretation of true positive P-glycoprotein expression when using myosin-containing tissues or tissues known to have p185 c-erbB2 expression.
Product DetailsProduct Details
- Verified Reactivity
- Human, Mouse
- Reported Reactivity
- Rat, Dog, Non-Human Primate
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- This antibody was made against SDS-solubilized plasma membranes of a multidrug resistant Chinese hamster ovary (CHO) cell line and a human cell line.
- Formulation
-
Phosphate-buffered solution with BSA + 0.09% NaN3.
Previous lots of this product may have been formulated with 0.1% or 0.05% NaN3 instead of 0.09% NaN3. For further information please contact BioLegend Technical Support or Customer Service. - Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
- Storage & Handling
- Store between 2°C and 8°C.
- Application
-
IHC-P - Quality tested
WB - Verified
IHC-F, ICC, FC, WB, ELISA - Reported in the literature, not verified in house - Recommended Usage
-
Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a dilution range of 1:20 - 1:40 is suggested. For western blotting, the suggested use of this reagent is a 1:100 dilution. It is recommended that the reagent be titrated for optimal performance for each application.
- Application References
-
- Hisano Y, et al. 2011. J. Biol. Chem. 286: 1758. (WB)
- Beck WT, et al. 1996. Cancer Res. 56:3010. (IHC-P, IHC-F, ICC, FC) PubMed
- Evans CH, Baker PD. 1992. Cancer Res. 52: 5893. (WB, FC)
- Toth K, et al. 1992. Am J Pathol. 140:1009. (IHC-P, ICC, WB)
- Finstad CL, et al. 1991. J Histochem Cytochem. 39:1603. (FC, WB, IHC-P
- Georges E, et al. 1990. PNAS. 87:152. (ELISA, IHC-P)
- Duan Z, et al. 2014. BMC Cancer. 14:681. (WB)
- Inui N, et al. 2012. J Gerontol A Biol Sci Med Sci. 67:1049. (FC, IF)
- Erickson MA, et al. 2012. J Neuroinflammation. 9:150. (WB)
- Rao VV, et al. 1994. Cancer Res. 54:1536. (WB) PubMed
- Lee JJ, et al. 1996. Cancer. 77:1892. (IHC) PubMed
- Product Citations
-
- RRID
-
AB_2565033 (BioLegend Cat. No. 903701)
Antigen Details
- Biology Area
- Immunology
- Molecular Family
- Adhesion Molecules, CD Molecules
- Gene ID
- 5244 View all products for this Gene ID
- UniProt
- View information about CD243/ABCB4 on UniProt.org
Related Pages & Pathways
Pages
Other Formats
View All CD243/ABCB4 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-CD243/ABCB4 (ABCB1, P-glycoprotein1/MDR3) | C219 | IHC-P,WB,IHC-F,ICC,ELISA,FC |
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Purified anti-CD243/ABCB4 (ABCB1, P-glycoprotein1/MDR3)
IHC staining of purified anti-CD243/ABCB4 (ABCB1, P-glycopro... Whole cell extracts (15 µg total protein) from the indicated... Whole cell extracts (15 µg total protein) from the indicated...
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