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Purified anti-CD68 Antibody

Pricing & Availability
Clone
KP1 (See other available formats)
Regulatory Status
RUO
Other Names
Macrosialin, CD68 antigen, macrophage antigen CD68, scavenger receptor class D, member 1
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
KP1_PURE_CD68_1_Antibody_100517
IHC staining of anti-CD68 antibody (clone KP1) on formalin-fixed paraffin-embedded Alzheimer´s disease human brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with the primary antibody at 5 µg/ml overnight at 4°C. BioLegend's Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. Images were captured with a 40X objective. Scale Bar: 20 µm
  • KP1_PURE_CD68_1_Antibody_100517
    IHC staining of anti-CD68 antibody (clone KP1) on formalin-fixed paraffin-embedded Alzheimer´s disease human brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with the primary antibody at 5 µg/ml overnight at 4°C. BioLegend's Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. Images were captured with a 40X objective. Scale Bar: 20 µm
  • KP1_PURE_CD68_2_Antibody_100517
    IHC staining of anti-CD68 antibody (clone KP1) on formalin-fixed paraffin-embedded Parkinson´s disease human brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with the primary antibody at 5 µg/ml overnight at 4°C. BioLegend's Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. Images were captured with a 40X objective.
  • KP1_PURE_CD68_3_Antibody_041816
    IHC staining of anti-CD68 antibody (clone KP1) on formalin-fixed paraffin-embedded human tonsil tissue. Following antigen retrieval using Retrieve-All Antigen Unmasking System 1 (Cat. No. 927901), the tissue was incubated with the primary antibody at 0.2 µg/ml for 60 minutes at room temperature. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided.
  • KP1_PURE_CD68_4_Antibody_010325
    Jurkat cells (negative cell line, panel A) and THP-1 cells (positive cell line, panel B) were fixed with 4% PFA Fixation Buffer (Cat. No. 420801) for 10 minutes and permeabilized with 100% ice-cold methanol for 10 minutes, and blocked with 5% FBS for 1 hour at room temperature. Cells were then stained with Purified anti-CD68 (clone KP1), followed by incubation with Alexa Fluor® 594 Goat anti-mouse IgG (Cat. No. 405326) for 1 hour at room temperature. Nuclei were counterstained with DAPI (Cat. No. 422801). The images were captured on a Revvity Operetta CLS™ High Content Analysis System with a 63X objective and merged. Scale bar: 50 µm
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916104 100 µg 100€
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Description

Mouse CD68, also known as macrosialin, is an 85-115 kD member of the lysosomal-associated membrane protein (LAMP) family. It is a heavily glycosylated and predominantly intracellular protein, mainly in late endosomes. Macrosialin is the murine homolog to the human macrophage glycoprotein CD68. It is expressed on tissue macrophages, Langerhans cells and at low levels on dendritic cells. High levels of CD68 expression are detected in activated microglia. LAMP proteins may have functions related to cell-cell interaction or cell-ligand interaction. The biological function of CD68 is not completely understood.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
This antibody was generated using a Lysosomal fraction of human alveolar macrophages.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

IHC-P - Quality tested
ICC - Verified
IP, WB - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 0.2 - 5.0 μg/mL is suggested. For immunocytochemistry, a concentration range of 1.25 – 10.0 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

For use in immunocytochemistry (ICC), it is recommended to fix/permeabilize with either of the following:

- Fixation buffer (Cat. No. 420801) with 100% ice-cold methanol

- 100% ice-cold methanol only

Fixation/permeabilization with Fixation buffer (Cat. No. 420801) followed by 0.5 % Triton X-100 is not recommended due to non-specific staining.

Application References
  1. Holness CL, et al. 1993. Blood 81(6):1607.
  2. Horny HP, et al. 1990. J. Clin. Path. 43:719.
  3. Pulford KAF, et al. 1989. J. Clin. Path. 42:414.
Product Citations
  1. Altorki NK, et al. 2022. Cell Rep. 39:110639. PubMed
  2. McMahon NP, et al. 2023. Cancers (Basel). 15: . PubMed
  3. Van Slambrouck J, et al. 2023. EBioMedicine. 92:104608. PubMed
  4. Wagner J et al. 2019. Cell. 177(5):1330-1345 . PubMed
  5. Kiniwa T, et al. 2016. Proc Natl Acad Sci U S A. 113: 10139 - 10144. PubMed
  6. Gide TN, et al. 2019. Cancer Cell. 35:238. PubMed
  7. Burlingame EA, et al. 2021. Cell Rep Methods. 1:. PubMed
  8. Gudjonsson JE, et al. 2020. JCI Insight. 5:. PubMed
  9. Xie S, et al. 2020. Ann Transl Med. 1.384027778. PubMed
  10. Wang C, et al. 2022. Signal Transduct Target Ther. 7:289. PubMed
  11. Chevrier S, et al. 2018. Cell Syst. 0.675. PubMed
  12. Johnson BE, et al. 2022. Cell Rep Med. 3:100525. PubMed
RRID
AB_2616797 (BioLegend Cat. No. 916104)

Antigen Details

Structure
Sialomucin family, 110 kD.
Distribution

Microglia, monocytes, macrophages, dendritic cells, granulocytes, subset of hematopoietic progenitors, γ/δ T cells, NK cells, LAK cells, subset of B cells, fibroblasts, and endothelial cells.

Cell Type
B cells, Dendritic cells, Endothelial cells, Fibroblasts, Granulocytes, Hematopoietic stem and progenitors, Macrophages, Microglia, Monocytes
Biology Area
Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers, Signal Transduction
Molecular Family
CD Molecules
Gene ID
968 View all products for this Gene ID
UniProt
View information about CD68 on UniProt.org
Go To Top Version: 2    Revision Date: 01.03.2025

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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