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Purified anti-CK1α Antibody

Pricing & Availability
Clone
W18288B (See other available formats)
Regulatory Status
RUO
Other Names
CSNK1A1, CK1, CK1a, HLCDGP1, PRO2975, HEL-S-77p, Casein Kinase 1 Alpha 1, Casein Kinase I Isoform Alpha, Epididymis Secretory Sperm Binding Protein Li 77p
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
W18288B_PURE_CK1a_Antibody_081020.png
Whole cell extracts (15 µg total protein) from the indicated cell lines were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1.0 µg/mL (1:500 dilution) of purified anti-CK1α antibody (clone W18288B) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-rat IgG antibody (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH antibody (Cat. No. 607904) was used as a loading control at a 1:25000 dilution (lower). Lane M: Molecular weight marker.
  • W18288B_PURE_CK1a_Antibody_081020.png
    Whole cell extracts (15 µg total protein) from the indicated cell lines were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1.0 µg/mL (1:500 dilution) of purified anti-CK1α antibody (clone W18288B) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-rat IgG antibody (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH antibody (Cat. No. 607904) was used as a loading control at a 1:25000 dilution (lower). Lane M: Molecular weight marker.
Cat # Size Price Quantity Check Availability Save
938201 25 µg 81€
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938202 100 µg 203€
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Description

Casein Kinase 1 Alpha 1 (CK1α) is a member of the CK1 family of serine/threonine kinases which phosphorylate various regulatory molecules. CK1α is one of seven different non-allelic casein kinase isoforms, each with distinct signaling partners conferred by the sequence deviation in their C-terminal extensions. It plays a critical role in Wnt/β-catenin signaling through phosphorylation of β-catenin at serine 45 by GSK-3, which primes β-catenin for additional phosphorylation events, and is a critical component of the Axin-GSK-3-β-catenin scaffold. Through its phosphorylation of tumor suppressor p53 at serine 20, CK1α plays an important role in cell cycle regulation, DNA repair, apoptosis, and tumor suppression, thus making it of particular interest as a therapeutic target. Studies suggest that CK1α interacts with keratin-associated protein FAM83H to mediate the organization of keratin filaments and cytoskeletal organization.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Full-length recombinant human CK1α protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.125 - 1.0 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This clone was tested for ICC using HeLa cells fixed with 4% paraformaldehyde and permeabilized with either methanol or Triton X-100. Neither method resulted in CK1a staining.

This clone was tested for IP using whole cell extracts prepared from HeLa cells. The clone failed to immunoprecipitate CK1a.

This clone recognizes multiple isoforms of CK1a.

RRID
AB_2876759 (BioLegend Cat. No. 938201)
AB_2876759 (BioLegend Cat. No. 938202)

Antigen Details

Structure
CK1α is a 337 amino acid protein with a predicted molecular weight of 39 kD. It also exists as 365 and 325 amino acid isoforms with predicted molecular weights of 42 and 38 kD, respectively.
Distribution

Ubiquitously expressed/ Cytosolic

Function
Serine/threonine protein kinase
Biology Area
Cell Biology
Molecular Family
Protein Kinases/Phosphatase
Antigen References
  1. Jiang S, et al. 2018. Cell Commun. Signal. 16:23.
  2. Kuga T, et al. 2013. J. Cell Sci. 126:4721.
  3. Macdonald B, et al. 2009. Dev. Cell. 17:9.
  4. Schittek B, and Sinnberg T. 2014. Mol. Cancer. 13:231
Gene ID
1452 View all products for this Gene ID
UniProt
View information about CK1alpha on UniProt.org
Go To Top Version: 1    Revision Date: 08.10.2020

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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