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Purified anti-FABP5 Antibody

Pricing & Availability
Clone
CPTC-FABP5-1 (See other available formats)
Regulatory Status
RUO
Other Names
Fatty acid-binding protein 5 (FABP5), Epidermal-type fatty acid-binding protein (E-FABP), Psoriasis-associated fatty acid-binding protein homolog (PA-FABP)
Isotype
Mouse IgG1
Ave. Rating
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Product Citations
publications
a. CPTC-FABP5-1_PURE_FABP5_WB_040323
Whole cell extracts (15 µg total protein) from indicated tissues were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 0.25 µg/mL of purified anti-FABP5 (clone CPTC-FABP5-1) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG (Cat. No. 405306). Direct-Blot™ HRP anti-β-actin (Cat. No. 664804) was used as a loading control at a 1:50000 dilution. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Lane M: Molecular weight marker
  • a. CPTC-FABP5-1_PURE_FABP5_WB_040323
    Whole cell extracts (15 µg total protein) from indicated tissues were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 0.25 µg/mL of purified anti-FABP5 (clone CPTC-FABP5-1) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG (Cat. No. 405306). Direct-Blot™ HRP anti-β-actin (Cat. No. 664804) was used as a loading control at a 1:50000 dilution. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Lane M: Molecular weight marker
  • b. CPTC-FABP5-1_PURE_FABP5_IHC-P-1_040323
    IHC staining of purified anti-FABP5 (clone CPTC-FABP5-1) on formalin-fixed paraffin-embedded mouse brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R. (Cat. No. 928502), the tissue was incubated with 0.25 µg/mL of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin HRP Kit (Multi-Species, DAB)(Cat. No. 929501) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • CPTC-FABP5-1_PURE_FABP5_IHC-P-2_040323
    IHC staining of purified anti-FABP5 (clone CPTC-FABP5-1) on formalin-fixed paraffin-embedded human brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R. (Cat. No. 928502), the tissue was incubated with 0.25 µg/mL of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin HRP Kit (Multi-Species, DAB)(Cat. No. 929501) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
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613951 25 µg 96€
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613952 100 µg 332€
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Description

Fatty Acid Binding Protein 5 (FABP5) is a protein that plays an important role in the regulation of cellular fatty acid metabolism. Initially discovered in epidermal cells, FABP5 binds long-chain fatty acids and other related hydrophobic molecules to facilitate their uptake into the cell. Utilizing this transport function, FABP5 plays an important role in regulating fatty acid metabolism and energy homeostasis. Additionally, FABP5 is capable of transporting lipid-based signaling molecules such as endocannabinoids, functioning in diverse pathways such as glucose homeostasis, retinoic acid signaling, and the inflammatory response. 

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant full-length human FABP5
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
IHC-P - Verified
Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.25 - 1.0 µg/mL. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 0.25 - 1.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

 For IHC-P, titration to concentrations below 0.25 µg/mL may improve results.

 

RRID
AB_2941614 (BioLegend Cat. No. 613951)
AB_2941614 (BioLegend Cat. No. 613952)

Antigen Details

Structure
FABP5 is a 135 amino-acid protein with a predicted molecular weight of 15.1 kD.
Distribution

Plasma membrane, keratinocytes and epithelial cells

Function
Transporter
Ligand/Receptor
Long-chain fatty acids and related lipids
Cell Type
Epithelial cells
Biology Area
Neuroscience, Signal Transduction
Molecular Family
Neurotransmitter Receptor Trafficking/Endocytosis
Antigen References
  1. Hotamisligil GS and Bernlohr DA. 2015. Nat Rev Endocrinol. 11:592-605.
  2. Nowowiejska J, et al. 2022. Metabolites. 12:833.
  3. Xu B, et al. 2022. Front Cell Dev Biol. 10:857919. 
Gene ID
2171 View all products for this Gene ID
UniProt
View information about FABP5 on UniProt.org
Go To Top Version: 1    Revision Date: 04.03.2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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