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Purified anti-GFAP Antibody

Pricing & Availability
Clone
SMI 21 (See other available formats)
Regulatory Status
RUO
Other Names
Glial fibrillary acidic protein
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
SMI-21_Purified_GFAP_Antibody_1_011719.png
IHC staining of purified anti-GFAP antibody (clone SMI 21) on formalin-fixed paraffin-embedded monkey brain tissue. Following antigen retrieval using Retrieve-All Antigen Unmasking System 3: Acidic, 100X (Cat. No. 927601), the tissue was incubated with 1 µg/ml of the primary antibody for 60 minutes at room temperature. BioLegend's Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • SMI-21_Purified_GFAP_Antibody_1_011719.png
    IHC staining of purified anti-GFAP antibody (clone SMI 21) on formalin-fixed paraffin-embedded monkey brain tissue. Following antigen retrieval using Retrieve-All Antigen Unmasking System 3: Acidic, 100X (Cat. No. 927601), the tissue was incubated with 1 µg/ml of the primary antibody for 60 minutes at room temperature. BioLegend's Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • SMI-21_Purified_GFAP_Antibody_2_011719.png
    IHC staining of purified anti-GFAP antibody (clone SMI 21) on formalin-fixed paraffin-embedded canine brain tissue. Following antigen retrieval using Retrieve-All Antigen Unmasking System 3: Acidic, 100X (Cat. No. 927601), the tissue was incubated with 1 µg/ml of the primary antibody for 60 minutes at room temperature. BioLegend's Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • SMI-21_Purified_GFAP_Antibody_3_011719.png
    IHC staining of purified anti-GFAP antibody (clone SMI 21) on formalin-fixed paraffin-embedded human brain tissue. Following antigen retrieval using Retrieve-All Antigen Unmasking System 3: Acidic, 100X (Cat. No. 927601), the tissue was incubated with 1 µg/ml of the primary antibody for 60 minutes at room temperature. BioLegend's Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • SMI-21_Purified_GFAP_Antibody_4_011719.png
    Western blot of purified anti-GFAP antibody (clone SMI 21). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain lysate. The blot was incubated with 1 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
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837204 100 µg 221€
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Description

Glial fibrillary acidic protein (GFAP) is an intermediate filament (IF) protein that is expressed by numerous cell types of the central nervous system (CNS) including astrocytes and ependymal cells. GFAP has also been found to be expressed in glomeruli and peritubular fibroblasts, Leydig cells of the testis, keratinocytes, osteocytes and chondrocytes and stellate cells of the pancreas and liver. GFAP is a type III IF protein that is closely related to its non-epithelial family members, vimentin, desmin, and peripherin, which are all involved in the structure and function of the cell’s cytoskeleton. GFAP is thought to help to maintain astrocyte mechanical strength, as well as the shape of cells.

Type III intermediate filaments are highly conserved and contain three domains, named the head, rod and tail domains. This rod domain coils around that of another filament to form a dimer, with the N-terminal and C-terminal of each filament aligned. Type III filaments such as GFAP are capable of forming both homodimers and heterodimers; GFAP can polymerize with other type III proteins or with neurofilament light chain protein (NF-L). Interestingly, GFAP and other type III IF proteins cannot assemble with keratins, the type I and II intermediate filaments; in cells that express both proteins, two separate intermediate filament networks form.

To form networks, the initial GFAP dimers combine to make staggered tetramers, which are the basic subunits of an intermediate filament. The non-helical head and tail domains are necessary for filament formation. The head and tail regions have greater variability of sequence and structure. In spite of this increased variability, the head of GFAP contains two conserved arginines and an aromatic residue that are required for proper assembly.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Non-Human Primate, Dog
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human brain micro vessels.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

IHC-P - Quality tested
WB - Verified
ICC - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 1.0 - 10 μg/ml is suggested. For Western blotting, the suggested use of this reagent is 1 - 10 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

SMI 21 has been tested for immunocytochemical localization of GFAP in astrocytes and Bergmann glia in human, monkey and dog paraffin sections. Astrocytomas from human and dog show a positive reaction with SMI 21. Rat, rabbit and mouse brain sections do not react with SMI 21. Other species have not been examined. SMI 21 shows no cross reactivity with other intermediate filaments in tissue sections or Western blot lysates derived from human or rat brain tissue.

Application References
  1. Perng MD, et al. 2008. Mol. Biol. Cell. 19:4521. (ICC, WB) PubMed
  2. Chen MH, et al. 2013. ASN Neuro. 5:AN20130032. (ICC, WB) PubMed
  3. Chort A, et al. 2013. Brain. 136:1732. (IHC-P, WB) PubMed
Product Citations
  1. Turovsky EA, et al. 2021. International Journal of Molecular Sciences. 22(11):. PubMed
  2. Turovsky EA, et al. 2021. International Journal of Molecular Sciences. 22(16):. PubMed
RRID
AB_2632644 (BioLegend Cat. No. 837204)

Antigen Details

Structure
GFAP is a 432 amino acid protein with a molecular mass of approximately 50 kD.
Distribution

Tissue distribution: brain, cerebral cortex, hippocampus, cerebellum, and spinal cord. GFAP is expressed by numerous cell types of the central nervous system (CNS) including astrocytes, ependymal cells, and Bergmann glia cells (protoplasmic astrocyte). GFAP is expressed in cells lacking fibronectin.
Cellular distribution: cytoskeleton and cytosol.

Biology Area
Cell Biology, Neuroscience, Neuroscience Cell Markers
Molecular Family
Intermediate Filaments
Antigen References

1. Khakh BS, Sofroniew MV. 2015. Nat. Neurosci. 18:942-52. PubMed

Gene ID
2670 View all products for this Gene ID
UniProt
View information about GFAP on UniProt.org

Other Formats

View All GFAP Reagents Request Custom Conjugation
Description Clone Applications
Anti-GFAP SMI 21 IHC-P,WB,ICC
Purified anti-GFAP SMI 21 IHC-P,WB,ICC
Go To Top Version: 3    Revision Date: 09.22.2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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