- Clone
- N355/1 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- GluA1/GluR1 glutamate receptor, Glutamate receptor 1, GluR1, gluR-1, gluR-K1, glutamate receptor A, glutamate receptor subunit GluR1, AMPA-selective glutamate receptor 1, glutamate receptor, ionotropic, AMPA1 (alpha 1)
- Previously
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Covance Catalog# MMS-5143
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![N355slash1_Purified_GluR1_Antibody_1_012615 N355slash1_Purified_GluR1_Antibody_1_012615](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/N355slash1_Purified_GluR1_Antibody_1_031618.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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Western blot of purified anti-GluR1 antibody (clone N355/1). Lane 1: Molecular weight marker; Lane 2: 20 µg of mouse brain membrane lysate; Lane 3: 20 µg of rat brain membrane lysate. The blot was incubated with 10 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system. -
IHC staining of purified anti-GluR1 antibody (clone N355/1) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 10 µg/ml of the primary antibody overnight at 4°C. BioLegend´s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm -
IHC staining of purified anti-GluR1 antibody (clone N355/1) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 10 µg/ml of the primary antibody overnight at 4°C. BioLegend´s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective.
Cat # | Size | Price | Quantity Check Availability | Save | ||
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819802 | 25 µL | 67€ | ||||
819801 | 100 µL | 132€ |
GluR1 belongs to the family of Glutamate Receptors (GluRs) that are essential for regulating excitatory synaptic transmission by L-glutamate in the CNS of vertebrates. These are mostly expressed in granule and pyramidal cells in the hippocampus. GluRs can be categorized as ionotropic or metabotropic and subcategorized by their specific agonists (NMDA, AMPA or Kainic acid). There are four types of AMPA related GluR subunits (GluR1, GluR2, GluR3 and GluR4) that are generated by alternative RNA splicing. Tetrameric or pentameric combinations of different subunits contribute to the functional diversity of AMPA receptors.
Product DetailsProduct Details
- Verified Reactivity
- Mouse, Rat
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- This monoclonal antibody was raised against a fusion protein corresponding to amino acids 1-389 (extracellular, N-terminus) of rat GluA1/GluR1 glutamate receptor.
- Formulation
- Phosphate-buffered solution.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 1 mg/ml
- Storage & Handling
- This antibody should be handled aseptically as it is free of preservatives such as Sodium Azide. Store this antibody undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check the vial label or CoA to find the proper storage conditions.
- Application
-
WB - Quality tested
IHC-P - Verified - Recommended Usage
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Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 5.0 - 10.0 µg per ml. For immunohistochemistry of formalin-fixed paraffin-embedded tissue, a concentration of 10.0 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
- Product Citations
-
- RRID
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AB_2734581 (BioLegend Cat. No. 819802)
AB_2564834 (BioLegend Cat. No. 819801)
Antigen Details
- Structure
- Expected MW: 100 kD
- Cell Type
- Glutamatergic Neurons
- Biology Area
- Cell Biology, Neuroscience, Neuroscience Cell Markers, Synaptic Biology
- Molecular Family
- Postsynaptic proteins
- Gene ID
- 50592 View all products for this Gene ID
- UniProt
- View information about GluA1/GluR1 glutamate receptor on UniProt.org
Related Pages & Pathways
Pages
Related FAQs
Other Formats
View All GluA1/GluR1 glutamate receptor Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-GluR1 | N355/1 | WB,IHC-P |
Biotin anti-GluR1 | N355/1 | IHC-P |
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Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-GluR1
Western blot of purified anti-GluR1 antibody (clone N355/1).... IHC staining of purified anti-GluR1 antibody (clone N355/1) ... IHC staining of purified anti-GluR1 antibody (clone N355/1) ... -
Biotin anti-GluR1
IHC staining of Biotin anti-GluR1 antibody (N355/1) on forma... IHC staining of Biotin anti-GluR1 antibody (N355/1) on forma... IHC staining of Biotin anti-GluR1 antibody (N355/1) on forma... IHC staining of Biotin anti-GluR1 antibody (N355/1) on forma...
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