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Purified anti-HSP60 Antibody

Pricing & Availability
Clone
P83G8 (See other available formats)
Regulatory Status
RUO
Other Names
HSPD1, HLD4, CPN60, GROEL, HSP65, SPG13, HuCHA60, Mitochondrial Matrix Protein P1, P60 Lymphocyte Protein
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
P83G8_PURE_HSP60_Antibody_1_WB_072116
Total cell lysate from HeLa cells (lane 1, 15 µg), NIH3T3 cells (lane 2, 15 µg) and mouse brain (lane 3, 15 µg) were resolved by electrophoresis (4-12% Tris-Glycine gel), transferred to nitrocellulose, and probed with purified anti-HSP60 (clone P83G8) antibody. Proteins were visualized using an HRP Goat anti-mouse IgG Antibody and chemiluminescence detection. Purified anti-β-actin antibody (poly6221) was used as a loading control.
  • P83G8_PURE_HSP60_Antibody_1_WB_072116
    Total cell lysate from HeLa cells (lane 1, 15 µg), NIH3T3 cells (lane 2, 15 µg) and mouse brain (lane 3, 15 µg) were resolved by electrophoresis (4-12% Tris-Glycine gel), transferred to nitrocellulose, and probed with purified anti-HSP60 (clone P83G8) antibody. Proteins were visualized using an HRP Goat anti-mouse IgG Antibody and chemiluminescence detection. Purified anti-β-actin antibody (poly6221) was used as a loading control.
  • P83G8_PURE_HSP60_Antibody_2_ICC_041619
    A549 cells were fixed with 4% paraformaldehyde (PFA) for ten minutes and then were permeabilized with 0.5% Triton X-100 for five minutes followed by blocking with 5% FBS for 30 minutes. Then the cells were intracellularly stained with 2.5 µg/mL anti-HSP60 (clone P83G8) in blocking buffer overnight at 4°C and followed by DyLight™ 594 (red) conjugated goat anti-mouse IgG for one hour at room temperature. Actin filaments were labeled with Alexa Fluor® 488 Phalloidin (green). Nuclei were counterstained with DAPI (blue). The image was captured with a 60X objective.
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681502 100 µg 231€
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Description

HSP60, a mitochondrial chaperonin, is essential for the refolding, assembly, and transportation from the cytoplasm to mitochondrial matrix. It shares a high degree of homology and function similarity to bacteria GroEL. Studies also show that HSP60 may participate in some autoimmune diseases along with autosomal dominant and autosomal recessive neurodegenerative disorders.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Purified recombinant fragment (265-462 a.a.) of human HSP60 expressed in E. coli.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
ICC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.5 µg per ml. For immunocytochemistry, a concentration range of 1.0 - 10 μg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Product Citations
  1. Di Gioia M, et al. 2020. Nat Immunol. 21:42. PubMed
RRID
AB_2566474 (BioLegend Cat. No. 681502)

Antigen Details

Structure
HSP60 contains 573 amino acids with a predicted molecular weight of 61 kD.
Distribution

Mitochondrial matrix.

Function
HSP60 plays important roles in trafficking, folding, and the assembly of mitochondrial proteins. Under stress conditions, it may prevent protein misfolding and aggregation.
Interaction
Interacts with HRAS (by similarity), HBV protein X, HTLV-1 protein p40tax, ATAD3A, METTL20, and METTL21B.
Ligand/Receptor
TLR4.
Biology Area
Cell Biology, Immunology, Innate Immunity, Mitochondrial Function, Neuroinflammation, Neuroscience, Neuroscience Cell Markers
Molecular Family
Mitochondrial Markers
Antigen References

1. Bukau B, Horwich AL. 1998. Cell 92:351.
2. Hansen JJ, et al. 2002. Am. J. Hum. Genet. 70:1328.
3. Kol A, et al. 2000. J. Immunol. 164:13.
4. Jindal S, et al. 1989. Mol. Cell Biol. 9:2279.
5. Magen D, et al. 2008. Am. J. Hum. Genet. 83:30.
6. Chandra D, et al. 2007. J. Biol. Chem. 282:31289.

Gene ID
3329 View all products for this Gene ID
UniProt
View information about HSP60 on UniProt.org
Go To Top Version: 3    Revision Date: 06.10.2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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