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Purified anti-human CD163 Antibody

Pricing & Availability
Clone
RM3/1 (See other available formats)
Regulatory Status
RUO
Other Names
GHI/61, M130, RM3/1, p155, Hemoglobin/haptoglobin complex receptor, Macrophage-associated antigen, ED2(rat), Macrophage marker
Isotype
Mouse IgG1, κ
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Product Citations
publications
RM3slash1_Purified_CD163_Antibody_FC_032014
IL-10-stimulated (overnight) human peripheral blood monocytes were stained with purified CD163 (clone RM3/1, filled histogram) or purified mouse IgG1, κ isotype control (open histogram), followed by anti-mouse IgG FITC.
  • RM3slash1_Purified_CD163_Antibody_FC_032014
    IL-10-stimulated (overnight) human peripheral blood monocytes were stained with purified CD163 (clone RM3/1, filled histogram) or purified mouse IgG1, κ isotype control (open histogram), followed by anti-mouse IgG FITC.
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326502 100 µg 95€
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Description

CD163 is a member of the group B scavenger receptor cysteine-rich superfamily, also known as GHI/61, M130, RM3/1, p155, hemoglobin-haptoglobin complex receptor, or macrophage-associated antigen. It is a 134 kD (non-reduced)/155 kD (reduced) glycoprotein primarily expressed on macrophages, Kuffer cells, monocytes, subset of dendritic cells, and a subset of hematopoietic stem/progenitor cells. CD163 binds to haptoglobin-hemoglobin complex and TWEAK, and plays a role in clearing hemoglobin and regulating cytokine production by macrophages. Membrane CD163 can be cleaved by metalloproteinases (MMP), resulting in a soluble form. Elevated serum level of sCD163 has been implicated in many kinds of inflammation diseases.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human monocytes
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
ICC - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 2.0 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Clone RM3/1 binds to domain 9 of CD163. Additional reported applications (for the relevant formats) include: immunofluorescence7.

Application References
  1. Högger P, et al. 1998. J. Immunol. 161:1883. (FC)
  2. Zwadlo G, et al. 1987. Exp. Cell Biol. 55:295. (FC)
  3. Buechler C, et al. 2000. J. Leukoc. Biol. 67:97. (FC)
  4. Puig-Kroger A, et al. 2009. Cancer Res. 69:9395. (FC) PubMed
  5. Madsen M, et al. 2004. J. Biol. Chem. 279:51561. (FC)
  6. Jones K, et al. 2013. Clin Cancer Res. 19:731. (FC) PubMed
  7. Stewart DA, et al. 2012. Mol. Cancer Res. 10:727. (IF)
Product Citations
  1. Talbot H, et al. 2020. Sci Rep. 10:12572. PubMed
  2. Stewart D, et al. 2012. Mol Cancer Res. 0.921527778. PubMed
  3. Cho H, et al. 2007. Physiol Genomics. 29:149. PubMed
RRID
AB_893267 (BioLegend Cat. No. 326502)

Antigen Details

Structure
134 kD (non-reduced)/155 kD (reduced) glycoprotein, Scavenger receptor superfamily
Distribution

Monocytes, macrophages, Kuffer cells, subset of dendritic cells, subset of hematopoietic stem/progenitor cells

Function
Clearance of haptoglobin-hemoglobin complex, regulation of cytokine production by macrophages
Ligand/Receptor
Haptoglobin-hemoglobin complex, TWEAK
Cell Type
Dendritic cells, Hematopoietic stem and progenitors, Macrophages, Monocytes
Biology Area
Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers
Molecular Family
CD Molecules
Antigen References

1. Roth J, et al. 1994. Transolantation. 57:127.
2. Van den Heuvel MM, et al.1999. J. Leukoc. Biol. 66:858.
3. Sulahian TH, et al. 2000. Cytokines 12:1312.
4. Fabriek BO, et al. 2007. J. Neuroimmunol. 187:179.

Gene ID
9332 View all products for this Gene ID
UniProt
View information about CD163 on UniProt.org
Go To Top Version: 4    Revision Date: 05.07.2014

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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