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Purified anti-human CD179b (Ig λ5) Antibody

Pricing & Availability
Clone
HSL11 (See other available formats)
Regulatory Status
RUO
Other Names
Ig Lambda 5, λ5, Ig omega
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
HSL11_PE_081210
Human cell lines REH (pro-B, blue), NALM-6 (pre-B, orange) and Ramos (B cell, open) stained with purified HSL11 conjugated with PE
  • HSL11_PE_081210
    Human cell lines REH (pro-B, blue), NALM-6 (pre-B, orange) and Ramos (B cell, open) stained with purified HSL11 conjugated with PE
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349802 100 µg 141€
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Description

CD179b, also known as Ig Lambda 5, λ5, and Ig omega, is a 22 kD, C-like protein member within the Ig superfamily. λ5 associates with CD179a (VpreB) to form a surrogate light (SL) chain that in turn associates with the IgM heavy chain and the CD79a/CD79b heterodimer to form the pre-B cell receptor (PreBCR) complex. The function of CD179a/CD179b is still under investigation, though it has been reported that the deficiency of CD179a and/or CD179b impairs the maturation of the B cell precursors beyond the pre-B cell stage.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Reported Reactivity
African Green
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
ICFC, IHC-P, IHC-F, IP - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation1 and immunohistochemistry of frozen and paraffin-embedded samples2.

Application References
  1. Tsyganezawa K, et al. 1998. Blood 92:4317. (IP)
  2. Kiyokawa N, et al. 2004. Modern Pathol. 17:423. (ICFC, IHC)
RRID
AB_10644164 (BioLegend Cat. No. 349802)

Antigen Details

Structure
Immunoglobine (Ig) V-like protein. Molecular weight 22 kD. Associates with CD179a (VpreB) to form the surrogate light (SL) chain, which binds to the the Ig µ heavy chain and CD79a/CD79b heterodimer complex to form the pre-B cell receptor (preBCR).
Distribution

Pro-B and early Pre-B cells

Cell Type
B cells
Biology Area
Cell Biology, Costimulatory Molecules, Immunology, Signal Transduction
Molecular Family
CD Molecules
Antigen References

1. Zola H, et al. 2007. Leukocyte and Stromal Cell Molecules:The CD Markers Wiley-Liss A John Wiley & Sons Inc.
2. Zhang M, et al. 2004. Cell. Mol. Immunol. 1:89.
3. Hendriks RW and Middendorp S. 2004. Trends Immunol. 25:249.

Gene ID
3543 View all products for this Gene ID
UniProt
View information about CD179b on UniProt.org
Go To Top Version: 1    Revision Date: 11.30.2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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