- Clone
- ITEM-1 (See other available formats)
- Regulatory Status
- RUO
- Workshop
- HCDM listed
- Other Names
- Fibroblast growth factor-inducible 14 kD protein (Fn14), TNFRSF12A
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
314002 | 100 µg | 184€ |
TWEAK receptor is also known as fibroblast growth factor inducible 14 kD protein (Fn14) or CD266. It is a 14 kD transmembrane protein that is expressed on a variety of tissue types. This receptor binds TWEAK and interacts with the cytoplasmic proteins TRAF1, 2, and 3. The TWEAK receptor is rapidly induced during liver regeneration and m play a role in hepatocyte growth. TWEAK receptor engagement can induce cell death and may play a role in inflammation. The ITEM-1 antibody is useful for flow cytometric analysis, induction of tumor cells apoptosis or necrotic cell death, enhancing endothelial cell proliferation, and immunohistochemistry on frozen tissues.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Human Fn14 transfected P815
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
FC - Quality tested
Apop, WB, IHC-P - Reported in the literature, not verified in house - Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
Additional reported applications (for the relevant formats) include: Western blotting2, immunohistochemistry of paraformaldehyde-fixed paraffin-embedded sections3, and in vitro induction of caspase-dependent cell death1,2. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 314006).
- Application References
-
- Nakayama M, et al. 2003. J. Immunol. 170:341. (Apop)
- Felli N, et al. 2005. J. Immunol. 175:1464. (Apop, WB)
- Muñoz-García B, et al. 2006. Stroke. 37:2044. (IHC)
- RRID
-
AB_345406 (BioLegend Cat. No. 314002)
Antigen Details
- Structure
- Transmembrane TNF superfamily receptor, contains no death domain, 14 kD
- Distribution
-
Widely expressed, high constitutive expression in heart placenta, lung, muscle, kidney, pancreas, and HUVEC
- Function
- Binds to TWEAK (CD255), thought to play a role in cell death and inflammation
- Ligand/Receptor
- TWEAK (CD255); interacts with cytoplasmic proteins TRAF1, 2, and 3
- Biology Area
- Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Immunology
- Molecular Family
- CD Molecules, Cytokine/Chemokine Receptors
- Antigen References
-
1. Feng SL, et al. 2000. Am. J. Path. 156:1253.
2. Meighan-Mantha RL, et al. 1999. J. Biol. Chem. 274:33166.
3. Nakayama M, et al. 2003. J. Immunol. 170:341.
4. Lynch CN, et al. 1999. J. Biol. Chem. 274:8455. - Gene ID
- 51330 View all products for this Gene ID
- UniProt
- View information about CD266 on UniProt.org
Other Formats
View All CD266 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-human CD266 (Fn14, TWEAK R) | ITEM-1 | FC,Apop,WB,IHC-P |
PE anti-human CD266 (Fn14, TWEAK R) | ITEM-1 | FC |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
-
Purified anti-human CD266 (Fn14, TWEAK R)
Human Fn14 transfected cells stained with purified ITEM-1, f... -
PE anti-human CD266 (Fn14, TWEAK R)
Human Fn14 transfected cells stained with ITEM-1 PE
Follow Us