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Purified anti-CD326 (EpCAM) Antibody

Pricing & Availability
Clone
W21090A (See other available formats)
Regulatory Status
RUO
Other Names
Ep-CAM, tumor associated calcium signal transducer 1, epithelial cell surface antigen, epithelial glycoprotein 2, EGP2, adenocarcinoma associated antigen, TROP1
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
A. W21090A_PURE_CD326_IHC-P_091523
IHC staining using purified anti-CD326 (EpCAM) (clone W21090A) on formalin-fixed paraffin-embedded human colon tissue. Following antigen retrieval using 1X Citrate buffer (Cat. No. 420901), the tissues were incubated with 5 µg/mL of purified anti-CD326 (EpCAM) (W21090A) overnight at 4°C, followed by incubation with 2.5 µg/mL of Alexa Fluor® 647 Goat anti-rat IgG (red) (Cat. No. 405416) for one hour at room temperature. Slide was mounted with ProLong™ Gold Antifade Mountant. Nuclei were counterstained with DAPI (blue) (Cat. No. 422801). The image was captured with a 40X objective. Scale bar: 25 µM
  • A. W21090A_PURE_CD326_IHC-P_091523
    IHC staining using purified anti-CD326 (EpCAM) (clone W21090A) on formalin-fixed paraffin-embedded human colon tissue. Following antigen retrieval using 1X Citrate buffer (Cat. No. 420901), the tissues were incubated with 5 µg/mL of purified anti-CD326 (EpCAM) (W21090A) overnight at 4°C, followed by incubation with 2.5 µg/mL of Alexa Fluor® 647 Goat anti-rat IgG (red) (Cat. No. 405416) for one hour at room temperature. Slide was mounted with ProLong™ Gold Antifade Mountant. Nuclei were counterstained with DAPI (blue) (Cat. No. 422801). The image was captured with a 40X objective. Scale bar: 25 µM
  • B. W21090A_PURE_CD326_ICC_091523
    ICC staining of purified anti-CD326 (EpCAM) (clone W21090A). HT-29 (panels A and B) and HeLa cells (panels C and D) were fixed with ice-cold methanol for 10 minutes and blocked with 5% FBS for 1 hour at room temperature. Cells were then intracellularly stained with 5.0 µg/mL of purified anti-CD326 (EpCAM) (clone W21090A), followed by incubation with 2.5 µg/mL of Alexa Fluor® 488 Goat anti-rat IgG (green) (Cat. No. 405418) for 1 hour at room temperature. Nuclei were counterstained with DAPI (blue) (Cat. No. 422801), and the images were captured with a 40X objective. Scale bar: 25 µM
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624151 25 µg 136€
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624152 100 µg 344€
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Description

CD326 is also known as EpCAM (Epithelial cell adhesion molecule), a glycosylated I transmembrane protein containing six disulfide bridges and one THYRO domain. It is highly expressed in bone marrow, colon, lung, and most normal epithelial cells where it functions as an adhesion molecule. In addition to cell adhesion, CD326 is also involved in cellular signaling, cell migration, proliferation, and differentiation. Recently, it has been reported that CD326 expression occurs during the early steps of erythrogenesis. CD326 functions as a homotypic calcium-independent cell adhesion molecule and is involved in carcinogenesis via its ability to induce genes c-myc, e-fabp, and cyclins A & E involved in cellular metabolism and proliferation. Research studies have demonstrated that CD326 plays a role in tumorigenesis and metastasis of carcinomas, therefore, has the potential to serve as a prognostic marker and an immunotherapeutic target.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Extracellular domain of recombinant human CD326 protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

IHC-P - Quality tested
ICC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 1.0 - 10.0 µg/mL is suggested. For immunocytochemistry, a concentration range of 1.0 - 5.0 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This clone does not cross-react with mouse.

For immunocytochemistry (ICC), we recommend using ice-cold methanol fixation.

For immunohistochemistry (IHC-P), the suggested antigen retrieval buffer is citrate buffer (Ca. No. 420901) or Tris-EDTA pH 9.0.

RRID
AB_3097236 (BioLegend Cat. No. 624151)
AB_3097236 (BioLegend Cat. No. 624152)

Antigen Details

Structure
CD326 is an 314 amino acid protein with a predicted molecular weight of 35 kD
Distribution

Highly expressed in bone marrow, colon, lung, and most normal epithelial cells. Also highly expressed on carcinomas of gastrointestinal origin. Expressed during early erythrogenesis.

Function
Homotypic calcium-independent cell adhesion. CD326 is involved in carcinogenesis by its ability to induce genes involved in cellular metabolism and proliferation.
Interaction
Monomer. Interacts with phosphorylated CLDN7
Ligand/Receptor
LAIR-1 (CD305), LAIR-2 (CD306), Ep-CAM
Cell Type
Epithelial cells
Biology Area
Cancer Biomarkers, Cell Biology, Cell Proliferation and Viability, Immunology
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References
  1. Strnad J, et al. 1989. Cancer Res. 49:314.
  2. Munz M, et al. 2004. Oncogene. 23:5748.
  3. Maetzel D, et al. 2009. Nature Cell Biology. 11:162–71.
  4. Osta WA, et al. 2004. Cancer Res. 64:5818–24.
  5. Litvinov SV, et al. 1996. Journal of Pathology. 148:865–75.
  6. Armstrong A, et al. 1996. Cancer Biology & Therapy. 2:320–6.
  7. Rao CG, et al. 2005. Int J Oncol. 27:49.
Gene ID
4072 View all products for this Gene ID
UniProt
View information about CD326 on UniProt.org
Go To Top Version: 1    Revision Date: 09.15.2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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