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Purified anti-human CD68 Recombinant Antibody

Pricing & Availability
Clone
QA20B07 (See other available formats)
Regulatory Status
RUO
Other Names
Macrosialin
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
QA20B07_PURE_CD68_Antibody_032922
Human paraffin-embedded tonsil tissue slices were prepared with a standard protocol of deparaffination and rehydration. Antigen retrieval was done with Citrate buffer 1X pH 6.0 at 95°C for 40 minutes. Tissue was washed with PBS/ 0.05% Tween-20 twice for five minutes, blocked with 5% FBS and 0.2% gelatin for 30 minutes and permeabilized with 0.5% Triton X-100. Then, the tissue was stained with 10 µg/mL of purified anti-human CD68 recombinant (clone QA20B07) and purified anti-human IgD (clone W18340A) at 4°C overnight. On the next day, the tissue was washed twice with PBS and stained with Alexa Fluor™ 594 goat anti-mouse IgG (clone Poly4053) (red) and Alexa Fluor™ 647 goat anti-rat IgG (clone Poly4054) (green) for two hours at room temperature. The nuclei were counter staining with DAPI (blue). The image was scanned with a 10X objective and stitched with software.
  • QA20B07_PURE_CD68_Antibody_032922
    Human paraffin-embedded tonsil tissue slices were prepared with a standard protocol of deparaffination and rehydration. Antigen retrieval was done with Citrate buffer 1X pH 6.0 at 95°C for 40 minutes. Tissue was washed with PBS/ 0.05% Tween-20 twice for five minutes, blocked with 5% FBS and 0.2% gelatin for 30 minutes and permeabilized with 0.5% Triton X-100. Then, the tissue was stained with 10 µg/mL of purified anti-human CD68 recombinant (clone QA20B07) and purified anti-human IgD (clone W18340A) at 4°C overnight. On the next day, the tissue was washed twice with PBS and stained with Alexa Fluor™ 594 goat anti-mouse IgG (clone Poly4053) (red) and Alexa Fluor™ 647 goat anti-rat IgG (clone Poly4054) (green) for two hours at room temperature. The nuclei were counter staining with DAPI (blue). The image was scanned with a 10X objective and stitched with software.
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381202 100 µg 90€
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Description

CD68, also known as macrosialin, is a 110 kD glycoprotein which belongs to the sialomucin family. It is closely related to the family of acidic, highly glycosylated lysosomal-associated membrane proteins (LAMPs). CD68 is predominately expressed in cytoplasmic granules of monocytes/macrophages, dendritic cells, and granulocytes. It is one of the useful myeloid cell markers. Further studies have shown that CD68 is also expressed by a subset of hematopoietic progenitors, γ/δ T cells, NK cells, LAK cells, subset of B cells, fibroblasts, and endothelial cells. The biological function of CD68 is still unknown.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Recombinant
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

IHC-P - Quality tested
IHC-F, IP - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 5 - 10 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2922613 (BioLegend Cat. No. 381202)

Antigen Details

Structure
Sialomucin family, 110 kD
Distribution

Monocytes/macrophages, dendritic cells, granulocytes, subset of hematopoietic progenitors, γ/δ T cells, NK cells, LAK cells, subset of B cells, fibroblasts, endothelial cells

Molecular Family
CD Molecules
Antigen References
  1. Holness CL and Simmons DL. 1993. Blood. 81:1607-13.
  2. Gottfried E, et al. 2008. Scand J Immunol. 67:453-63.
  3. Hameed A, et al. 1994. Hum Pathol. 25:872-6.
Gene ID
968 View all products for this Gene ID
UniProt
View information about CD68 on UniProt.org
Go To Top Version: 1    Revision Date: 03.29.2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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