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Purified anti-human IL-5 Antibody

Pricing & Availability
Clone
JES1-39D10 (See other available formats)
Regulatory Status
RUO
Other Names
Interleukin-5, Eosinophil differentiation factor (EDF), Eosinophil colony stimulating factor (Eo-CSF), B cell growth factor-2 (BCGF-2), B cell differentiation factor for IgM (BCDF-m), IgA enhancing factor (IgA-EF), T cell replacing factor-1 (TRF-1)
Isotype
Rat IgG2a, κ
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Product Citations
publications
Human_IL-5_020405
  • Human_IL-5_020405
Cat # Size Price Quantity Check Availability Save
500902 500 µg 210€
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Description

IL-5 is a homodimeric, disulphide-linked protein produced by T-cells. Monomeric human IL-5 is a 126 amino acid protein with a reported molecular weight of 26 kD for the homodimeric protein. Mouse and human IL-5 are approximately 70% identical. IL-5 has been shown to promote the growth of immature hematopoietic BFU-E progenitors, stimulate the activation and differentiation of eosinophils, and promote the generation of cytotoxic lymphocytes. The JES1-39D10 antibody can neutralize the bioactivity of natural or recombinant IL-5.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Reported Reactivity
Cynomolgus, Rhesus
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
COS-expressed, recombinant human IL-5
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

ELISA Capture - Quality tested
IHC-F, ICC, WB - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by ELISA assay. For ELISA capture applications, a concentration range of 1.0 - 4.0 µg/ml is recommended. To obtain a linear standard curve, serial dilutions of IL-5 recombinant protein ranging from 500 to 4 pg/ml are recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

ELISA1-3 or ELISPOT4-6 Capture: The purified JES1-39D10 antibody is useful as the capture antibody in a sandwich ELISA or ELISPOT, when used in conjunction with the biotinylated JES1-5A10 (Cat. No. 501002) antibody as the detecting antibody. The LEAF™ purified antibody is suggested for ELISPOT capture.
Neutralization1: The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for neutralization of human IL-4 bioactivity (Cat. No. 500907).
Additional reported applications (for the relevant formats) include: Western blotting, immunohistochemical staining8,9 of paraformaldehyde-fixed, saponin-treated frozen tissue sections, and immunocytochemistry.
Note: For testing human IL-5 in serum or plasma, BioLegend's ELISA Max™ Sets (Cat. No. 430401 & 430404) are specially developed and recommended.

Application References
  1. Abrams, J., et al. 1992. Immunol. Rev. 127:5.
  2. Butterfield, J., et al. 1992. Blood 79:688.
  3. Abrams, J., et al. 1994. Eosinophils in Allergy and Inflammation. Marcel Dekker, New York. p.133.
  4. Klinman, D., et al. 1994. Curr. Prot. Immunol.. John Wiley and Sons, New York. Unit 6.19.6.
  5. Mahanty, S., et al. 1992. J. Immunol. 148:3567.
  6. Limaye, A., et al. 1991. J. Clin. Inv. 88:1418.
  7. Prussin, C., et al. 1995. J. Immunol. Meth. 188:117.
  8. Andersson, U., et al. 1999. Detection and quantification of gene expression. New York:Springer-Verlag.
  9. Andersson, J., et al. 1994. Immunology 83:16.
Product Citations
  1. Tripathi T, et al. 2019. Immunohorizons. 0.201388889. PubMed
RRID
AB_315137 (BioLegend Cat. No. 500902)

Antigen Details

Structure
Cytokine; dimer; 45 kD (Mammalian)
Bioactivity
Growth/differentiation of eosinophils; proliferation/cell activation/differentiation of eosinophilic granulocytes
Cell Sources
Mast cells, T cells, bone marrow stromal cells
Cell Targets
Eosinophils
Receptors
Heterodimer IL-5Rα (CD125); β-subunit (CDw131) in common with IL-3R, GM-CSFR
Biology Area
Immunology
Molecular Family
Cytokines/Chemokines
Antigen References

1. Fitzgerald, K., et al. Eds. 2001. The Cytokine FactsBook. Academic Press, San Diego.
2. Takatsu, K., et al. 1988. Immunol. Rev. 102:107.
3. Takatsu, K. 1992. Curr. Opin. Immunol. 4:299.
4. Takatsu, K. 1991. Microbiol. Immun. 35:593.

Regulation
Upregulated by IL-2, platelet activating factor; downregulated by TGF-β
Gene ID
3567 View all products for this Gene ID
UniProt
View information about IL-5 on UniProt.org

Other Formats

View All IL-5 Reagents Request Custom Conjugation
Description Clone Applications
PE anti-human IL-5 JES1-39D10 ICFC
Purified anti-human IL-5 JES1-39D10 ELISA Capture,IHC-F,ICC,WB
Go To Top Version: 2    Revision Date: 08.01.2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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