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Purified anti-human Integrin α9β1 Antibody

Pricing & Availability
Clone
Y9A2 (See other available formats)
Regulatory Status
RUO
Other Names
ITGA9, ITGB1
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
Y9A2_Pure_082911
Human peripheral blood granulocytes were stained with purified integrin α9β1 (clone Y9A2) (filled histogram) or mouse IgG1, κ isotype control (open histogram), followed by anti-mouse IgG-PE.
  • Y9A2_Pure_082911
    Human peripheral blood granulocytes were stained with purified integrin α9β1 (clone Y9A2) (filled histogram) or mouse IgG1, κ isotype control (open histogram), followed by anti-mouse IgG-PE.
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351602 100 µg 184€
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Description

Integrin α9β1 is a transmembrane protein belonging to the integrin family. It is widely expressed in airway epithelium, different types of muscle cells, and hepatocytes. In the hematopoietic system, integrin α9β1 is highly expressed on human neutrophils and at low level on monocytes. Multiple ligands have been identified for this integrin including the inducible endothelial counter-receptor, vascular cell adhesion molecule-1 (VCAM-1), the extracellular matrix proteins tenascin C and osteopontin, some members of ADAMs family, coagulation factor XIII, and von Willebrand factor. It has been reported that binding of the α9 cytoplasmic domain to spermidine/spermine N(1)-acetyltransferase (SSAT) mediates α9β1 enhancement of cell migration, mediates adhesion to the extracellular matrix proteins, and is involved in G-CSF signaling. More recently, integrin α9β1 was found on hematopoietic stem and progenitor cells to mediate stem cells adhesion, proliferation, and differentiation.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Reported Reactivity
African Green, Baboon, Cynomolgus, Rhesus
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
L-cells transfected with human α9
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
ICC, IP - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported application (for the relevant formats) include: immunofluorescence microscopy1, immunopricipitation2,  and blocking3.

Application References
  1. Lafuste P, et al. 2005. Mol. Biol. Cell. 16:861. (IF)
  2. Chen C, et al. 2006. Immunity 25:895. (FC, IP)
  3. Taooka Y, et al. 1999. J. Cell Biol. 145:413. (Block)
Product Citations
  1. Mashinchian O, et al. 2022. EMBO J. 41:e110655. PubMed
RRID
AB_10901165 (BioLegend Cat. No. 351602)
Disclaimer

This product is for in vitro research use only. It is not to be used for commercial purposes. Use of this product to produce products for sale or for diagnostic, therapeutic or drug discovery purposes is prohibited. In order to obtain a license to use this product for commercial purposes, contact The Regents of the University of California.

Antigen Details

Structure
Heterodimeric proteins made up of α9 and β1 subunits
Distribution

Neutrophil, monocytes, endothelial cells, hepatopoietic cells, and different type of muscle cells

Function
Receptor for VCAM-1, involved in cell adhesion and migration
Interaction
Recognizes α9 integrin expressed as a heterodimer in association with the β1 integrin.
Ligand/Receptor
VCAM-1, ECM proteins tenascin C, osteopontin
Cell Targets
Binds VCAM-1
Cell Type
Endothelial cells, Monocytes, Neutrophils
Biology Area
Cell Adhesion, Cell Biology, Immunology, Innate Immunity, Neuroscience, Synaptic Biology
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Schreiber TD, et al. 2009 Haematologica 94:1495.
2. Chen C, et al. 2006 Immunity 25:895.
3. Taooka Y, et al. 1999 J. Cell Biol. 145:413.
4. Palmer EL, et al. 1993 J. Cell Biol. 123:1289.

Gene ID
3680 View all products for this Gene ID 3688 View all products for this Gene ID
UniProt
View information about Integrin alpha9beta1 on UniProt.org
Go To Top Version: 3    Revision Date: 09.06.2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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